Anti-proliferation effects of isorhamnetin on lung cancer cells in vitro and in vivo.

Background: Isorhamnetin (Iso), a novel and essential monomer derived from total flavones of Hippophae rhamnoides that has long been used as a traditional Chinese medicine for angina pectoris and acute myocardial infarction, has also shown a spectrum of antitumor activity. However, little is known about the mechanisms of action Iso on cancer cells.

Objectives: To investigate the effects of Iso on A549 lung cancer cells and underlying mechanisms.

No association between hypertension and risk for Alzheimer's disease: a meta-analysis of longitudinal studies.

This study examined the association between hypertension and AD by using a quantitative meta-analysis of longitudinal studies. EMBASE and MEDLINE were searched for articles published up to February 2011. All studies that examined the association of hypertension or antihypertensive medication use with the onset of AD were included.

Gene therapy with recombinant adenovirus encoding endostatin encapsulated in cationic liposome in coxsackievirus and adenovirus receptor-deficient colon carcinoma murine models.

Adenovirus (Ad)-based antiangiogenesis gene therapy is a promising approach for cancer treatment. Downregulation or loss of coxsackievirus and adenovirus receptor (CAR) is often detected in various human cancers, which hampers adenoviral gene therapy approaches. Cationic liposome-complexed adenoviral vectors have been proven useful in CAR-deficient cells to enhance therapeutic gene transfer in vivo.

[Relationship between polymorphism of hypoxia inducible factor-1alpha and cervical cancer in Han population in Sichuan Province of China].

Objective: To investigate the relationship between single nucleotide polymorphism (SNP) of hypoxia inducible factor-1alpha (HIF-1alpha) C1772T and genetic susceptibility to and clinical-pathological features of cervical cancers in Han population in Sichuan province of China.

Methods: A case control study was undertaken in Sichuan province of China, with 97 patients with uterine cervical cancer as case group and 117 negative for intraepithelial lesion or malignancy (NILM) patients as control group. Their gene types in HIF-1alpha C1772T were identified with a combination of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

[The influence of circadian clock gene on mouse sperm fertility in RNAi].

Objective: To study the influence of circadian Clock gene on the fertilization ability of sperm via attenuating the expression of Clock with RNAi in male mice.

Methods: After the injection of RNAi plasmid into mice testes, the expression of circadian Clock gene of testis tissue was determined by western Blotting. The fertilization ability of sperm was evaluated by various indices, including litter size in mated female mice, sperm count, sperm motility, in vitro fertilization (IVF) rate and acrosome development.

[Effects of circadian gene mPer1 on therapeutic sensitivity of adriamycin].

Objective: To study the effects of mPer1 gene on the response of mammary carcinoma EMT6 cells to Adriamycin in vitro.

Methods: The eukaryotic expression vector pcDNA3. 1 (+)-mPer1 was transfected into the EMT6 cells (EMT6-mPerl).

[Influence of heart rate's circadian rhythm in postoperation of intracerebral hemorrhage with different surgery].

Objective: To study the influence of different surgery on heart rate's circadian rhythm in postoperation of intracerebral hemorrhage.

Methods: One hundren cases of hypertensive intracerebral hemorrhage at basal ganglia had been collected. According to methods of operation, cases were divided into two groups.

[Identification of RACK1-PER1 protein interaction sites].

Objective: By screening the cDNA library of suprachiasmatic nucleus (SCN) region of human hypothalamus, we try to capture the novel proteins interacting with PER1 and investigate the interplaying characteristic of RACK1 and PER1.

Methods: By yeast two-hybrid system, the new protein in human SCN, which was associating with PER1-PAS domain was obtained. Then five truncated fragments of RACK1 cDNA was cloned into yeast expression vector to form recombinant library plasmid and was co-transformed into yeast strain AH109 with bait plasmid containing PER1-PAS domain.

[Inhibitory effects of deoxyribozyme on the expression of Period1 gene in vitro and on morphine-induced psychic dependence in mice].

Objective: To investigate the regulatory effects of deoxyribozyme on the expression of Period 1 gene in vitro and on the morphine-induced psychic dependence in mice.

Methods: The specific deoxyribozymes toward Period 1 mRNA was designed by MFold analysis and synethsized chemically. By LipofectAMINE mediated DNA transfection technique, DRz164 and pcDNA3-Per1 were introduced into NIH3T3 cells.

[Design and application of biological rhythm automatic monitor and data analysis system].

Objective: To design and develop a biological rhythm monitor and data analysis system for studying biological rhythm in animals under different conditions.

Methods: The system was a distributed digital control system consisting of a computer and zeitgeber generators. Acquisition of data on animal activity and generation of zeitgeber were modularized to meet the multiple requirements of experiment.

[Cleavage of the deoxyribozyme targeting Period1 mRNA in vitro and its effects on morphine-induced reward in mice].

Objective: To study the cleavage of the deoxyribozyme targeting Period1 (Per1) mRNA in vitro and its effect on the opiate-induced reward in mice.

Method: The specific deoxyribozyme (DRz164) targeting Per1 mRNA was designed after MFold analysis and being synthesized chemically. The cleavage reactions containing DRz164 and Per1 RNA fragments transcripted in vitro were performed under certain conditions.

[The influence of circadian gene mPeriod2 on Lewis cancer cell].

Objective: To investigate the role of circadian gene mPeriod2 (mPer2) on tumor proliferation and apoptosis.

Method: The eukaryotic mPer2 expression vector (pcDNA 3.1-mPer2) based on pcDNA 3.

[Effects of different light-dark cycles on total and differential leukocyte counts as well as spontaneous locomotor activity in mice].

Objective: To investigate the effect of different light-dark cycles on total and differential counts of leukocyte as well as spontaneous locomotor activity in mice.

Method: 144 ICR mice were raised under different light-dark cycles including LD 7 h/7 h, LD 12 h/12 h and LD 16 h/16 h for 8 weeks. Blood samples were obtained by enucleation of eye for total and differential counts of leukocyte.

[The effect of ribozyme specially cleaving per1 mRNA on c-fos mRNA and its expression in hippocampus of morphine addicted mice].

Objective: To study the change of c-fos mRNA and protein in hippocampus of morphine addicted mice after injected with ribozyme specially cleaving per1 mRNA.

Method: The recombined plasmid pcDNA 3.1-per1RZ DNA was injected into the ventricles of morphine addicted mice to transcript the corresponding ribozyme which cleaves per1 mRNA particularly.

[The role of circadian gene period1 in morphine reward in mice].

Objective: To investigate the role of circadian gene Period1 on drug dependence.

Method: The ribozyme cleave the Period1 mRNA (per1RZ) was designed and the per1RZ expression vector (pcDNA 3.1-per1RZ) was constructed based on eukaryotic expression vector pcDNA 3.

[Effects of different light-dark cycle on learning and memory in mice].

Objective: To study the effect of different light-dark cycles on learning and memory in mice.

Method: Seventy-two ICR mice were raised under different light-dark cycles including LD 5h/5h, LD 12h/12h and LD 22h/22h for 6 weeks. The locomotor activity was recorded continuously.