Two-stage orthogonality based estimation for semiparametric varying-coefficient models and its applications in analyzing AIDS data.

Semiparametric smoothing methods are usually used to model longitudinal data, and the interest is to improve efficiency for regression coefficients. This paper is concerned with the estimation in semiparametric varying-coefficient models (SVCMs) for longitudinal data. By the orthogonal projection method, local linear technique, quasi-score estimation, and quasi-maximum likelihood estimation, we propose a two-stage orthogonality-based method to estimate parameter vector, coefficient function vector, and covariance function.

[Ear reconstruction after rupture of postauricular expanded flap].

Objective: To summarize the principles of ear reconstruction after rupture of postauricular expanded flap.

Methods: The rupture of the postauricular expanded flap was graded as I-IV degree. For flap rupture of I and II degree, the ear reconstruction was performed immediately with autogenous cartilage framework.

[Application of bi-pedicled frontal expanded flap for cervical cicatricial contracture].

Objective: To investigate the therapeutic effect of bi-pedicled frontal expanded flap for cervical cicatricial contracture.

Methods: Tissue expanders were implanted under frontal muscle. After expansion, the frontal flaps were designed based on bilateral superficial temporal vessels and were transferred to the neck wound.

[Study of methylation of promoter of EYA1 gene in microtia].

Objective: To explore the methylation of CpG islands in promoter of eye absent gene 1 (EYA1) in microtia.

Methods: The methylation of CpG islands in EYA1 gene in 64 microtias and 36 healthy controls were measured using the technique of matrix-assisted laser desorption/ionization-time of flight.

Results: The methylation of CpG_Unit3 and CpG_Unit5 of EYA1 gene in microtia were 0.

[Surgical treatment and etiological analysis of polyotia].

Objective: To explore the surgical treatment and pathologic mechanism of polyotia.

Methods: The operative methods for polyotia were flexible. The main procedures were aimed at the remodeling of tragus with the redundant tissue.

[Application of quantitative tissue expansion in ear reconstruction].

Objective: To explore the application of tissue expander in ear reconstruction and to deal with the complications.

Methods: 50 ml kidney-shape tissue expanders were implanted subcutaneously in the mastoid area. The drainage tube was removed 3 days after operation.

Comprehensive surgical approaches to management of various facial nevi.

Objective: To investigate the effects of the flexible surgical approaches on therapeutic and cosmetic outcomes of facial nevi.

Methods: From August 2002 to January 2008, 16 cases with facial nevi had been treated in our wards with the selected approaches. Surgical approaches including serial excision or one-time radical excision, free skin graft, and expanded flap were adopted in accordance with the individual size and location of the facial nevi.

[Clinical application of three methods for total ear reconstruction].

Objective: To investigate the indication and results of three methods for total ear reconstruction.

Methods: 960 cases of total ear defect were treated with skin expansion and autogenous rib cartilage framework (n = 786), or skin expansion and Medpor framework (n = 150), or ear prosthesis (n = 24). The indication and results of the three methods were analysed.

[The experimental study on the effect of CDMP-1 on proliferation of residual ear chondrocytes of microtia cultured in vitro].

Objective: To investigate the alternation of biological characteristics of residual ear chondrocytes when proliferating in vitro, and study the effects of CDMP-1 on proliferation and differentiation of residual ear chondrocytes.

Methods: Residual ear chondrocytes were cultured in vitro. Then we observed the effect of CDMP-1 on differentiation by immunohistochemistry of collagen type II and dyeing with toluidine blue, investigated proliferation effects of CDMP-1 by method of MIT, and analysis cell cycle changes by flow cytometry.

[Surgical treatment of acquired ear defect].

Objective: To explore surgical procedure for the treatment of acquired ear defect.

Methods: The ear reconstruction was carried out by using soft tissue skin expander and autogenous rib cartilage framework, Medpor framework, auricular prosthesis, and so on.

Results: The long-term follow-ups showed that the flap of reconstructed ear was ruddy, soft, with normal sensory function.

[Ear reconstruction using soft tissue expander in the treatment of congenital microtia].

Objective: To explore surgical procedure of congenital microtia.

Methods: Ear reconstruction was made using soft tissue skin expander and autogenous rib cartilage framework.

Results: Long time follow-up showed that the flap of reconstructed ear was ruddy, soft, with normal sensory function; cartilage framework had no degeneration, absorption and deformation.

[Clinical study of chest development after costal cartilage harvesting in children].

Objective: To evaluate the development of the chest wall after costal cartilage harvesting in children.

Methods: Thirty-nine patients, who underwent microtia reconstruction with costal cartilage grafts, were examined in the donor-site with physical examination and radiography. Forty normal children were selected as control.

[Correction of cupped ear by using a stainless steel wire suspension technique].

Objective: To evaluate a new reliable surgical technique for correction the congenital cupped ear.

Methods: With 96 patients, a stainless steel wire was introduced to suspend auricular cartilage on the pericranium after the cupped ear was corrected. The follow-ups were carried out after the operation.

[A study on the inhibiting effects of transforming growth factor-beta R II antibody on fibroblasts].

Objective: To investigate the inhibiting action of transforming growth factor-beta type R II receptor(TGF-beta R II) antibody to fibroblasts.

Methods: Four groups of cultured skin fibroblasts were treated with different dosages of TGF-beta R II antibody. Examinations were performed with HYP, MTT, microscope, and flow cytometry techniques.

[Treatment of helical keloid with retroauricular skin expansion].

Objective: To introduce a new technique for the treatment of helical keloid.

Methods: The procedure consisted of two steps. In the first step, a kidney-shaped expander of 50 ml was placed subcutaneously in the retroauricular area.