Iridium(III)-Catalyzed C-H Amidation/Cyclization of -Sulfoximines with -Alkoxyamides: Formation of Thiadiazine 1-Oxides.

The first example of Ir(III)-catalyzed C-H activation/cyclization with -alkoxyamides as amidation reagents to simultaneously form functionalized thiadiazine 1-oxide derivatives was developed. This one-pot cascade protocol tolerated diverse functional groups and readily constructed various heterocyclic frameworks in moderate to good yield.

Specific Synthesis of 3-Indole Derivatives via Rh(III)-Catalyzed Cascade Annulation between -Phenylbenzimidamides and Pyridotriazoles.

An efficient synthetic method to construct -indole derivatives has been successfully developed involving rhodium-catalyzed highly selective C-H bond activation of -phenylbenzimidamides and subsequent couplings with pyridotriazoles. This cascade approach features excellent chemoselectivity and unique of products containing quaternary carbon center with the pyridyl moiety.

Dragonfly wing decorated by gold nanoislands as flexible and stable substrates for surface-enhanced Raman scattering (SERS).

A flexible and stable biomimetic SERS substrate was successfully fabricated by depositing gold (Au) nanoislands on the dragonfly wings (DW) via a simple DC magnetron sputtering system. Characterizations of the Au/DW nanostructure indicated that the optimum Au/DW-45 (sputtering time was 45 min) substrate owns high sensitivity, good stability and outstanding reproducibility. The limit of detection (LOD) for Rhodamine 6 G (R6G) was as low as 10 M and enhancement factor (EF) was calculated to be 2.

Density-matrix evaluation of the enhancement to resonant Raman scattering and fluorescence of molecules confined in metallic nanoparticle dimers.

In the present work we study the surface-enhanced resonant Raman scattering (SERRS) and fluorescence (SEF) spectra of a general model molecule confined in metallic dimers consisting of Ag, Au and hybrid AuAg nanoparticles (NPs). The electromagnetic (EM) enhancement factors were simulated by the generalized Mie scatting method and the scattering cross section of the molecules were obtained by density-matrix calculations. The influence of the size of the NPs and the separation between the dimer on the Raman scattering and fluorescence were systematically studied and analyzed in detail.

Association of HLA-DRB1, HLA-DQB1 Polymorphisms with HPV 16 E6 Variants among Young Cervical Cancer Patients in China.

Potential correlation of human papillomavirus (HPV) 16 E6 variants and human leukocyte antigen (HLA) class II polymorphisms has been suggested in patients with cervical cancer, so far little information is available about the possible interaction between E6 variants and HLA class II variability during the obviously accelerated progression to cervical cancer in young women. In this study, we aimed to explore the association between the HPV16 E6 variants and HLA-DRB1, DQB1 alleles in a Chinese young cervical cancer population. The HLA-DRB1, HLA-DQB1 polymophisms were genotyped by low-resolution polymerase chain reaction (PCR) with sequence-specific primer.

Domain Motions and Functionally-Key Residues of L-Alanine Dehydrogenase Revealed by an Elastic Network Model.

Mycobacterium tuberculosis L-alanine dehydrogenase (L-MtAlaDH) plays an important role in catalyzing L-alanine to ammonia and pyruvate, which has been considered to be a potential target for tuberculosis treatment. In the present work, the functional domain motions encoded in the structure of L-MtAlaDH were investigated by using the Gaussian network model (GNM) and the anisotropy network model (ANM). The slowest modes for the open-apo and closed-holo structures of the enzyme show that the domain motions have a common hinge axis centered in residues Met133 and Met301.

Prediction of allosteric sites on protein surfaces with an elastic-network-model-based thermodynamic method.

Allostery is a rapid and efficient way in many biological processes to regulate protein functions, where binding of an effector at the allosteric site alters the activity and function at a distant active site. Allosteric regulation of protein biological functions provides a promising strategy for novel drug design. However, how to effectively identify the allosteric sites remains one of the major challenges for allosteric drug design.

[Analysis of the 4th generation outer space bred Angelica dahurica by FTIR spectroscopy].

The major components of the 4th generation outer space bred angelica and the ground group were determined and analyzed by Fourier transform infrared spectroscopy (FTIR) and second derivative spectrum, considering the large mutation of the plants with space mutagenesis. The results show that the content of the coumarin (1741 cm(-1)), which is the main active components of the space angelica dahurica increased, and the content of the protein (1 459, 1 419 cm(-1)) and the fat (930 cm(-1)) increased slightly, whereas the content of the starch and the dietary fiber reduced drastically. There are obvious differences between the peak values of the second derivative spectra of the plants, revealing that the outer space angelica dahurica contained amine component at 1 279 cm(-1).

[Analysis of atractylodes macrocephala koidz of spaceflight breeding with XRF and PXRD].

The contents of various elements in the fourth generation atractylodes macrocephala koidz cultivated by spaceflight breeding were analyzed and compared with those of the ground group on the whole jointly by XRF and PXRD. The results showed that the element Na and Ti could not be detected in the space group, and the levels of K and Mn were enhanced by 1.16 and 1.

[Application of XRF to the analysis of Scutellaria baicalensis with space mutagenesis].

X-ray fluorescence spectrum (XRF) analytic method was applied to the determination of the contents and varieties of mineral elements in the space flight mutagenesis breeding scutellaria baicalensis Georgi, and compared with the ground group which were planted and collected together under the same conditions, in an attempt to search for the influence of space environment upon mutation on scutellaria baicalensis Georgi. The result indicates that the varieties of the main mineral elements in the two samples are samely basically, therefore, the two samples have same absorption and enrichment capability of specific element, but the contents of elements Ca, Na, Zn and S increase by 0.3, 0.

[Comprehensive characterization of main components and microstructures in five different species of Salvia miltiorrhiza bge].

Studied with X-ray fluorescence spectrum analytic (XRF), powder X-ray diffraction (PXRD) and Fourier transform infrared spectroscopy (FTIR) for five different species of Salvia miltiorrhiza bge grew in Anguo, the intrinsic components and microstructures were determined and comprehensive characterization was carried out. The figure of comprehensive characterization can describe characteristics of intrinsic components from elements, crystal and amorphous active component, it can give an intuitive description for the same components and subtle differences in the intrinsic components of Salvia miltiorrhiza bge, which are different idioplasmatic and same species. It is the ideal method because of the simple preparation of sample, rapid and fine accurate measurement with reproducibility and objectivity, so that it can be used for breeding of fine varieties of Chinese medicinal materials, Chinese medicinal materials GAP guidance of growing conditions, medicinal origin characteristics, the intrinsic composition and microstructure characterization, quality control and authenticity of the identification.

[Research on the fourth generation of radix isatidis by FTIR].

FTIR spectra of fourth-generation space bred radix isatidis and FTIR spectra of radix isatidis in a ground group were determined on the whole. Comparative analysis was conducted on main constituents (components) to accumulate data for comprehensively understanding and thoroughly studying the inherent property, and to explore a method of evaluating the quality of space bred radix isatidis. The results showed that slight difference existed in the positions and shapes of the absorption peaks in FTIR spectra of samples between the two groups.

[Measurement and analysis of platycodon grandiflorum with space flight mutagenesis breeding by XRF].

X-ray fluorescence spectrum (XRF) analytic method was used to analyze and compare the contents of various mineral elements in the ground group platycodon grandiflorum, the comparison group platycodon grandiflorum and the fourth generation platycodon grandiflorum with space flight mutagenesis breeding. The result indicated that the contents of microelements Zn, Mn and Fe in the outer space group increase by 1.9, 2.

[Research on the fourth generation of Saposhnikovia divaricata by XRF].

To cultivate oriented new varieties, Saposhnikovia divaricata seeds were carried by Shenzhou "3" satellite and filtered when they were being brought back. In the present paper, X-ray fluorescence(XRF) was used to mensurate and analyze the contents of elements of the two samples from the ground group and the 4th generation of space Saposhnikovia divaricata (space group) that have advantages in branches and leaves and yields. The contents of the main mineral elements in the two samples are the same basically.

[Analysis and characterization of Belamcanda chinensis with space mutagenesis breeding by X-ray fluorescence analysis and X-ray diffraction].

The contents of various elements in the fourth generation Belamcanda chinensis (L.) DC. with space mutagenesis breeding were analyzed and characterized.

[Novel identification of donkeyhide glue by X-ray fluorescence analysis].

A new fast identification method for a Chinese patent medicine donkeyhide glue was established. Six samples from different producing areas were collected and determined by X-ray fluorescence analysis(XRF). Elements characteristic graphs were plotted and compared with those from the comparison sample.

[Analysis of the contents of elements in spirulina from different producing areas].

Five samples of spirulina from different producing areas were analyzed and characterized with X-ray fluorescence analysis. The contents of K, Na, Ca, Mg, Fe, Zn and Mn in spinrulina samples from different producing areas were determined. The results show that spinrulinas are rich in microelements that human body needs.