A Gram-staining-negative, dark pink, rod-shaped, amastigote and cellulose-degrading strain, designated H9, was isolated from intestinal contents of Nipponacmea schrenckii. The isolate was able to grow at 4-42 °C (optimum, 25 °C), at pH 6.5-9.
View Article and Find Full Text PDFTo overcome the trade-off challenge encountered in the engineering of alginate lyase AlyG2 from Seonamhaeicola algicola Gy8 and to expand its potential industrial applications, we devised a two-step strategy encompassing activity enhancement followed by thermal stability engineering. To enhance the specific activity of efficient AlyG2, we strategically substituted residues with bulky steric hindrance proximal to the active pocket with glycine or alanine. This led to the generation of three promising positive mutants, with particular emphasis on the T91S mutant, exhibiting a 1.
View Article and Find Full Text PDFThe antioxidant effect of probiotics has been widely recognized across the world, which is of great significance in food, medicine, and aquaculture. There are abundant marine microbial resources in the ocean, which provide a new space for humans to explore new probiotics. Previously, we reported on the anti-infective effects of ML1206, a potential marine probiotic.
View Article and Find Full Text PDFMost reports on signal peptides focus on their ability to affect the normal folding of proteins, thereby affecting their secreted expression, while few studies on its effects on enzymatic properties were published. Therefore, biochemical characterization and comparison of alginate lyase rALYI1/rALYI1-1 (rALYI1: without signal peptides; rALYI1-1:with signal peptides) were conducted in our study, and the results showed that the signal peptide affected the biochemical properties, especially in temperature and pH. rALYI1 (32.
View Article and Find Full Text PDFA Gram-stain-positive, orange-pigmented, rod-shaped and flagellated bacterial strain T12 was isolated from wetland soil in Kunyu Mountain Wetland in Yantai, China. The strain was able to grow at 15-40 °C (optimum 37 °C), at 0.0-9.
View Article and Find Full Text PDFStrain C17, a novel strain belonging to the phylum Actinobacteria, was isolated from a thermal power plant in Yantai, Shandong Province, China. Cells of strain C17 were Gram stain positive, aerobic, pink, non-motile and round with neat edges, showing optimum growth at 28 °C. Phylogenetically, strain C17 was a member of the class Actinobacteria, order Mycobacteriales, family Gordoniaceae.
View Article and Find Full Text PDFA Gram-stain-negative, pink-pigmented, aerobic, non-motile and rod-shaped bacterium, designated as strain H15, was isolated from Acmaea sp., collected from Weihai, Shandong Province, China. The novel isolate was able to grow at 4-37 °C (optimum 33 °C), pH 5.
View Article and Find Full Text PDFAlginate, the most abundant polysaccharides of brown algae, consists of various proportions of uronic acid epimers -L-guluronic acid (G) and -D-mannuronic acid (M). Alginate oligosaccharides (AOs), the degradation products of alginates, exhibit excellent bioactivities and a great potential for broad applications in pharmaceutical fields. Alginate lyases can degrade alginate to functional AOs with unsaturated bonds or monosaccharides, which can facilitate the biorefinery of brown algae.
View Article and Find Full Text PDFAntonie Van Leeuwenhoek
January 2022
A Gram-stain-negative, wheat, rod-shaped, non-motile, non-spore forming, and facultatively anaerobic bacterium strain, designated as PI, was isolated from saline silt samples collected in saltern in Yantai, Shandong, China. Growth was observed within the ranges 4-45 °C (optimally at 33 °C), pH 6.0-9.
View Article and Find Full Text PDFAntonie Van Leeuwenhoek
November 2021
A novel gram-negative, aerobic, pink, motile, gliding, rod-shaped bacterium, designated P51, was isolated from saline silt samples in Yantai, China. It was able to grow at 4-42 °C (optimum 33 °C), pH 4.0-9.
View Article and Find Full Text PDFA Gram-stain negative, non-flagellated, beige-pigmented, circular, catalase-positive, oxidase-positive bacterium, designated G4, was isolated from gut microflora of top shell (Trochus maculatus Linnaeus) collected from Diwanggong market, Weihai, People's Republic of China. The novel isolate was able to grow at 4-42 °C (optimum 25-33 °C), pH 7.0-9.
View Article and Find Full Text PDFThe gut microbiome composition is influenced by many factors including environmental exposures. Here, we investigated the effect of thirdhand cigarette smoke (THS) and exposure age on gut microbiome diversity. C57BL/6 mice were exposed to THS at human exposure relevant levels for three weeks during three different life stages: postnatal (0-3 weeks of age), pubescent (4-7 weeks of age), and adult (9-12 weeks of age), respectively.
View Article and Find Full Text PDFWith the widespread occurrence of aquaculture diseases and the broad application of antibiotics, drug-resistant pathogens have increasingly affected aquatic animals' health. Marine probiotics, which live under high pressure in a saltwater environment, show high potential as a substitute for antibiotics in the field of aquatic disease control. In this study, twenty strains of non-hemolytic bacteria were isolated from the intestine of wild oysters and perch, and a model of infected by was established.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
June 2019
A Gram-stain-negative, aerobic, yellow, non-motile, rod-shaped and alginate-degrading bacterium, designated Dm15, was isolated from marine alga collected in Weihai, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Dm15 represents a distinct line of the family . Strain Dm15 had the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour (96.
View Article and Find Full Text PDFAn amendment to this paper has been published and can be accessed via the original article.
View Article and Find Full Text PDF(liquorice) is a well-known medicinal plant. Its roots and rhizomes are used as the popular Chinese herbal medicine Gan-Cao. An ethanol extract of the aerial parts of showed antidiabetic effects on db/db mice.
View Article and Find Full Text PDFObjective: The Collaborative Cross (CC) is a mouse population model with diverse and reproducible genetic backgrounds used to identify novel disease models and genes that contribute to human disease. Since spontaneous tumour susceptibility in CC mice remains unexplored, we assessed tumour incidence and spectrum.
Design: We monitored 293 mice from 18 CC strains for tumour development.
Variations in oral bacterial communities have been linked to oral cancer suggesting that the oral microbiome is an etiological factor that can influence oral cancer development. The 4-nitroquinoline 1-oxide (4-NQO)-induced murine oral and esophageal cancer model is frequently used to assess the effects of preventive and/or therapeutic agents. We used this model to assess the impact of the microbiome on tumorigenesis using axenic (germ-free) and conventionally housed mice.
View Article and Find Full Text PDFHeavy ion radiotherapy has shown great promise for cancer therapy. Understanding the cellular response mechanism to heavy ion radiation is required to explore measures of overcoming devastating side effects. Here, we performed a quantitative proteomic analysis to investigate the mechanism of carbon ion irradiation on human AHH-1 lymphoblastoid cells.
View Article and Find Full Text PDFWorld J Pediatr
August 2016
Background: To observe the development of neonatal sleep among healthy infants of different conceptional age (CA) by analyzing the amplitude-integrated electroencephalography (aEEG) of their sleep-wake cycles (SWC).
Methods: Bedside aEEG monitoring was carried out for healthy newborns from 32 to 46 weeks CA between September 1, 2011 and August 30, 2012. For each aEEG tracing, mean duration of every complete SWC, number of SWC repetition within 12 hours, mean duration of each narrow and broadband of SWC, mean voltage of the upper edge and lower edge of SWC, mean bandwidth of SWC were counted and calculated.
A novel Gram-stain positive, endospore-forming bacterium, designated SA4(T), was isolated from a soil sample collected from an abandoned marine solar saltern at Wendeng, Shandong Province, PR China. Cells were observed to be rod shaped, alginase positive, catalase positive and motile. The strain was found to grow at temperatures ranging from 15 to 40 °C (optimum 35 °C), and pH 5.
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