Predictive factors of epiretinal membrane in complicated rhegmatogenous retinal detachment tamponaded with silicone oil.

Aim: To determine the incidence and predictive factors for epiretinal membrane (ERM) formation in eyes with complicated primary rhegmatogenous retinal detachment (RRD) tamponaded with silicone oil (SO).

Methods: This retrospective case-control study included 141 consecutive patients with (51 eyes) and without (90 eyes) ERM formation after primary pars plana vitrectomy (PPV) and SO tamponade for complicated RRD. The risk factors for ERM were assessed using logistic regression analysis.

Multimode imaging characteristics and treatment of uveal schwannoma.

Aim: To delineate the different imaging characteristics of uveal schwannoma from melanoma and discuss the optimal treatment strategy for intraocular schwannoma.

Methods: Case series of three patients diagnosed with intraocular schwannoma was collected at Zhongshan Ophthalmic Center, Guangzhou, China from July 2014 to December 2020. All the study patients underwent ultrasonography and magnetic resonance imaging (MRI).

Prevalence and characteristics of peripheral myopic retinopathy in Guangzhou office workers.

Aim: To determine the prevalence and characteristics of peripheral myopic retinopathy among a sample of Guangzhou office workers.

Methods: A cross-sectional study of Guangzhou Chinese office works in different departments and units of the Guangzhou Power Supply Bureau, China, in 2016. Myopic retinopathy was recorded and analyzed with a scanning laser ophthalmoscope and by slit-lamp microscopy combined with a three-mirror contact lens.

Rac1 modulates the vitreous-induced plasticity of mesenchymal movement in retinal pigment epithelial cells.

Background: The vitreous has been shown to induce epithelial-mesenchymal transdifferentiation because it induces fibroblast-like morphology, enhanced migration and invasion in retinal pigment epithelial cells in proliferative vitreoretinopathy. Rac1 is the principal mediator of cell migration. In the current study, the relationship between Rac1 and cell migration, and invasion in vitreous-transformed retinal pigment epithelial cells was investigated using NSC23766, a specific inhibitor of Rac guanosine-5'-triphosphatase activity, and the involvement of a Rac1 guanosine-5'-triphosphatase-dependent pathway was detected.

[Clinical efficacy and safety of FP-7 Ahmed glaucoma valve implantation in neurovascular glaucoma patients].

Objective: To evaluate the efficacy and safety of FP-7 Ahmed glaucoma valves (AGV) implantation in neurovascular glaucoma (NVG) as the first choice of surgery.

Methods: This retrospective, comparative case series study collected a total of 36 eyes of 36 patients with neurovascular glaucoma who underwent AGV implantation in Zhongshan Ophthalmic Center from January 2009 to June 2010. Change of intraocular pressure (IOP), the best corrected visual acuity, numbers of anti-glaucoma medication, success rate and postoperative complications were followed up at day 1, week 1, month 1, and every 3 months after surgery.

Pro370Leu myocilin mutation in a Chinese pedigree with juvenile-onset open angle glaucoma.

Purpose: To investigate the genotype and phenotype of juvenile-onset open angle glaucoma (JOAG) in a Chinese family (PN pedigree).

Methods: Each family member was comprehensively examined by an experienced ophthalmologist. The clinical characteristics of the family patients with JOAG were documented.

Comparison of FP-7 and S-2 Ahmed glaucoma valve implantation in refractory glaucoma patients for short-term follow-up.

Background: Ahmed glaucoma valves (AGV) has been used for decades, but there is no detailed report about the efficacy of AGV in Chinese glaucoma patients. This study aimed to compare the intraocular pressure (IOP) lowering efficacy and side effects of S-2 polypropylene and PF-7 silicone AGV implantation in Chinese refractory glaucoma patients.

Methods: Patients were divided into S-2 model AGV group and FP-7 model AGV group.

[Gene screening as the forewarning measure to predict glaucoma].

Objective: To investigate the effect of gene screening on forewarning and monitoring of familial open-angle glaucoma pedigree.

Methods: Comprehensive ophthalmic examinations were performed in all available family members. The genomic DNA was extracted from peripheral blood.

Pro370Leu MYOC gene mutation in a large Chinese family with juvenile-onset open angle glaucoma: correlation between genotype and phenotype.

Purpose: Glaucoma is the leading cause of irreversible blindness worldwide. Most of the cases are primary open angle glaucoma (POAG). POAG is a genetically heterogenous disease; autosomal dominance is the most frequent type of monogenic inheritance.

Analysis of MYOC gene mutation in a Chinese glaucoma family with primary open-angle glaucoma and primary congenital glaucoma.

Background: Glaucoma is one of the leading causes of blindness in the world. Primary open-angle glaucoma (POAG) and primary congenital glaucoma (PCG) are subtypes of glaucoma. Myocillin is the first gene identified to be involved in POAG.

[Anti-proliferative effect of herpes simplex virus thymidine kinase gene system on human Tenon capsule fibroblasts in vitro].

Objective: To observe anti-proliferative effect of herpes simplex virus thymidine kinase gene system (HSV-tk) on human Tenon capsule fibroblasts (HTFs) and it's mechanism.

Methods: Retroviral vector was constructed containing HSV-tk gene and transfer to packaging cell line PT67. The positive clones was selected with G418 and the supernatant was collected which contains virus particles.

[Preliminary study of inhibition of human Tenon's capsule fibroblasts in vitro by RNA interference targeting IKK-beta].

Objective: To determine whether small interference RNA (siRNA) targeting the inhibitor of kappaB kinase-beta (IKK-beta) could be used to suppress the IKK-beta expression, and inhibit the proliferation of human Tenon's capsule fibroblasts in vitro.

Methods: IKK-beta specific siRNA designed from the human gene sequence was transfected into the cultured human Tenon's capsule fibroblasts, and a non-targeted siRNA was transfected as a negative control. The mRNA of IKK-beta was assessed by reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression was determined by Western blotting.

[A preliminary study on gene mutation and its function in a large family (GZ.1 pedigree) with open-angle glaucoma].

Objective: To determine the causative mutation of myocilin gene and to investigate its pathogenic function in a large Chinese pedigree (GZ.1) with familial open-angle glaucoma.

Methods: Genome-wide scanning was performed and the Lod scores were calculated.

[Induction of epidermal stem cells of rhesus monkey into human conjunctival epithelial cells: an in vitro experiment].

Objective: To observe the possibility of inducing the epidermal stem cells of rhesus monkey into human conjunctival epithelial cells and to investigate the plasticity of epidermal stem cells.

Methods: Epidermal stem cells of rhesus monkey were cultured in vitro and separated with type IV collagen. The cells were analyzed before and after isolation with beta1 integrin and keratin 15 by flow cytometry.