MiR-15b can target insulin receptor to regulate hepatic insulin signaling in mice.

Now diabetes is growing to be a health problems globally. However, its specific pathogenesis still needs further exploration. Here we showed that miR-15b was upregulated in the palmitate-induced HepG2 cells and livers of hyperglycemic mice.

Hepatic miR‑215 target Rictor and modulation of hepatic insulin signalling in rats.

Increasing evidence has suggested that hepatic lipid accumulation is associated with hepatic insulin resistance; however, the underlying mechanism is yet to be determined. It was demonstrated that the levels of microRNA‑215 (miR‑215) expression in the liver of rats fed a high‑fat diet were significantly increased compared with rats on a control diet. Additionally, it was revealed via luciferase assays and western blotting that miR‑215 targets rapamycin‑insensitive companion of mammalian target of rapamycin (Rictor), an important protein in the hepatic insulin signalling pathway.

Inhibition of cytochrome P450 enzymes by rhein in rat liver microsomes.

Rhein, an active ingredient extensively found in plants such as Aloe, Cassitora L., rhubarb and so on, has been used for a long time in China. Pharmacological tests revealed that rhein not only had a strong antibacterial action, but also may be useful in cancer chemotherapy as a biochemical modulator.

[Temperament of asthmatic children and risk factors for asthma].

Objective: Asthma is considered as a typical psychosomatic disease. This study aimed to investigate the temperament of asthmatic children and risk factors for asthma.

Methods: Temperamental type and dimensionality were investigated by Carry Temperament Scale in 106 children with asthma.

The unfolding intermediate state of calf intestinal alkaline phosphatase during denaturation in guanidine solutions.

The equilibrium unfolding of calf intestinal alkaline phosphatase in guanidinium chloride (GdmCl) solutions was studied by following the fluorescence and ultraviolet difference spectra. At low concentrations of GdmCl (< 1.6 M), the fluorescence intensity decreased with a slight red shift of the emission maximum from 332 nm to 344 nm.

Effect of extraneous zinc on calf intestinal alkaline phosphatase.

The effect of extraneous zinc on calf intestinal alkaline phosphatase was studied for quick reversible binding and slow irreversible binding of zinc ions at various concentrations. Under the conditions of slow binding of zinc to CIP increasing Zn2+ (less than 1.0 mM, nM/nE 1.

[Simultaneous determination of three probe drugs by reversed-phase high performance liquid chromatography].

A reversed-phase high performance liquid chromatographic method was developed for the simultaneous quantitative determination of caffeine, dapsone and chlorzoxazone. The operation was carried out on a C18 column (250 mm x 4.6 mm i.