Discovering common pathogenetic processes between COVID-19 and sepsis by bioinformatics and system biology approach.

Corona Virus Disease 2019 (COVID-19), an acute respiratory infectious disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has spread rapidly worldwide, resulting in a pandemic with a high mortality rate. In clinical practice, we have noted that many critically ill or critically ill patients with COVID-19 present with typical sepsis-related clinical manifestations, including multiple organ dysfunction syndrome, coagulopathy, and septic shock. In addition, it has been demonstrated that severe COVID-19 has some pathological similarities with sepsis, such as cytokine storm, hypercoagulable state after blood balance is disrupted and neutrophil dysfunction.

[Inventory and Environmental Impact of VOCs Emission from Anthropogenic Source in Chang-Zhu-Tan Region].

Based on environmental statistical data and emission factor, an anthropogenic volatile organic compounds (VOCs) emission inventory was established for the Chang-Zhu-Tan region, and a grid with spatial resolution of 3 km×3 km was built according to the spatial feature data. Ozone formation potential (OFP) and secondary organic aerosol (SOA) formation potential of anthropogenic sources were also estimated. The results showed that the total anthropogenic VOCs emission was about 113.

[Anthropogenic Ammonia Emission Inventory and Its Spatial Distribution in Chang-Zhu-Tan Region].

Based on the best available activity data and emission factors, an anthropogenic emission inventory of NH3 was established for the Chang-Zhu-Tan region with spatial resolution of 3 km x 3 km. The results showed that the total quantity of NH3 discharged from anthropogenic sources in the Chang-Zhu-Tan region was 7.27 x 10(4) t, and the average intensity of NH3 emission was 2.

Identification of genes differentially expressed in human hepatocellular carcinoma by a modified suppression subtractive hybridization method.

To identify differentially expressed genes in human HCC in China, we applied a modified SSH method for cDNA subtraction. Such modification has made the method more effective for subtraction. We have obtained 36 and 24 differentially expressed cDNA fragments after modified SSH from 4 paired samples of human HCC and non-HCC tissues, respectively.

Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma.

To extend the search for hepatocellular carcinoma (HCC) associated antigens with immunogenicity for clinical applications, we constructed a cDNA expression library using resected human HCC tissue sample and screened it by serological analysis of recombinant cDNA expression library (SEREX) with autologous and allogeneic sera. A total of 24 distinct antigens were isolated and kinectin was the antigen most frequently identified. We found that kinectin was alternatively spliced at four sites and obtained all eight theoretical forms of variant, six by SEREX and two by RT-PCR, from the different splicing combinations of the last three sites.

Purification of heat shock protein 70-associated tumor peptides and their antitumor immunity to hepatoma in mice.

Aim: To purify the heat shock protein (HSP) 70-associated tumor peptides and to observe its non-MHC-I molecule restrictive antitumor effect.

Methods: By ConA-sepharose affinity chromatography, ADP-agarose affinity chromatography, and DEAE anion exchange chromatography, we were able to purify HSP70-associated peptides from mouse hepatoma (HCaF) cells treated in heat shock at 42 degrees. Specific active immunization and adoptive cellular immunization assay were adopted to observe the immunoprotective effect elicited by HSP70-associated peptide complexes isolated from HcaF.