Flumazenil-Insensitive Benzodiazepine Effects in Recombinant αβ and Neuronal GABA Receptors.

Gamma-aminobutyric acid, type A (GABA) receptors are complex heterogeneous pentamers with various drug binding sites. Several lines of evidence suggest that benzodiazepines modulate certain GABA receptors in a flumazenil-insensitive manner, possibly via binding sites other than the classical ones. However, GABA receptor subtypes that contain non-classical benzodiazepine binding sites are not systemically studied.

Distant metastasis risk and patterns of nasopharyngeal carcinoma in the era of IMRT: long-term results and benefits of chemotherapy.

Purpose: To report the distant metastasis (DM) risk and patterns for nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy (IMRT) and to analyze the benefits of chemotherapy based on DM risk.

Materials And Methods: 576 NPC patients were analyzed. The DM rates were calculated using the Kaplan-Meier method, and the log-rank test was used to compare differences.

[Clinical application of stand-alone MC+PEEK cage in the anterior cervical fusion].

Objective: To explore the effect of clinical application of stand-alone MC+PEEK cage in anterior cervical fusion.

Methods: From January 2011 to January 2014,50 patients were treated with the MC+PEEK cage filled with autogenous cancellous illic-bone graft after anterior cervical discectomy. There were 22 patients with cervical spondylosis,26 patients with traumatic cervical disc herniation, 2 patients with cervical instability in these patients.

Risk factors and prediction-score model for distant metastasis in nasopharyngeal carcinoma treated with intensity-modulated radiotherapy.

The objective of this study is to identify the risk factors and construct a prediction-score model for distant metastasis (DM) in nasopharyngeal carcinoma (NPC) patients treated with intensity-modulated radiotherapy (IMRT). A total of 520 nonmetastatic NPC patients were analysed retrospectively. The independent risk factors for DM were tested by multivariate Cox regression analysis.

[Effects of low temperature exposure on dermal microvascular endothelial cells function].

Objective: To explore the damage effects and expression of vascular endothelial growth factor (VEGF) exposed with different low-temperatures on rat dermal microvascular endothelial cells (DMVECs).

Methods: Primary DMVECs were obtained by discontinuous Percoll gradient centrifugation. The DMVECs were identified by phase contrast microscope and immunofluorescence studies for CD31 antigen.

[The damage effects of hypothermia combined with hypoxia on rat pulmonary microvascular endothelial cells].

Objective: To study the synergistic effects of hypothermia and hypoxia on the damage of pulmonary microvascular endothelial cells (PMVEC) in rat.

Methods: Primary PMVECs were obtained by complex phosphoesterasum digesting from isolated lung tissues of Wistar rats, the PMVECs were identified by phase contrast microscope and immunofluorescence studies for CD31 antigen and bandeiraea simplicifolia isolectin (BSI) binding test. Factorial design was adopted in trial according to hypothermia and hypoxia existing or not.

[Clinical application of MC + PEEK cage in traumatic cervical disc herniation].

Objective: To explore the clinical outcomes of surgical treatment for traumatic cervical disc herniation with MC + PEEK cage.

Methods: A total of 51 patients with traumatic cervical disc herniation in mono-segment were surgically treated. The patients in group A (n = 20) were treated by MC + PEEK cage while those in group B (n = 31) by anterior cervical plate with PEEK cage or titanium mesh.

[Evaluation of cobas 4800 high-risk HPV test as a tool in cervical cancer screening and cytology triage].

Objective: To evaluate the feasibility and reliability of cobas 4800 HPV test for cervical cancer screening and cytology referral.

Methods: cobas 4800 HPV test and hybrid capture 2 (HC-2) were used to detect high risk HPV DNA in 670 specimens of liquid-based cytology collected from three hospitals. The agreement between cobas and HC-2 tests was assessed.

[Protective effects of SUR2B/Kir6.1 potassium channel opener natakalim against RAVECs injuries induced by hypoxia].

Objective: To investigate the protective effects of natakalim against rat aortic vascular endothelial cells (RAVECs) injuries induced by hypoxia and its mechanisms.

Methods: Selecting RAVECs as a cell model injured by hypoxia, these RAVECs were divided into 5 groups: i.e.