[Occurrence and Risk Assessment of -nitrosamines and Their Precursors in Qingjiang River Basin].

-nitrosamines are a type of nitrogen-containing organic pollutant with high carcinogenicity and mutagenicity. In the main drinking water sources of small and medium-sized towns in China, the contamination levels of -nitrosamines remain unclear. In addition, there is still lack of research on the concentration of -nitrosamines and their precursors in tributary rivers.

[Efficiency of clear aligners in extrusion of posterior teeth evaluated by 3-dimensional model superimposition].

Purpose: To explore the efficiency of posterior teeth extrusion with clear aligners by 3-dimensional model superimposition, which provides a reference for the design of clinical programs.

Methods: We selected 24 patients with clear aligners whose posterior teeth were designed to extend more than 0.5 mm, and a total of 126 teeth were included.

Polymorphisms of the apolipoprotein E gene affect response to atorvastatin therapy in acute ischemic stroke.

Background: Polymorphisms of the apolipoprotein E (APOE) gene are related to the efficacy of statin therapy. The biological functions of the APOE subtypes determine the metabolism of blood plasma lipids and the progression of atherosclerosis. This study aimed to explore the impact of APOE gene polymorphisms on the effect of atorvastatin on lipid regulation and plaque stabilization.

Effects of Different Intervention Time Points of Early Rehabilitation on Patients with Acute Ischemic Stroke: A Single-Center, Randomized Control Study.

Objective: To investigate effects of different intervention time points of early rehabilitation on patients with acute ischemic stroke.

Methods: We enrolled patients diagnosed with acute ischemic stroke in our hospital's rehabilitation ward from November 2013 to December 2015. Patients were randomly assigned to an ultraearly rehabilitation program (started within 72 hours of onset) or an early rehabilitation program (started from 72 hours to 7 days after onset).

A Novel Clinical Nomogram to Predict Transient Symptomatic Associated with Infarction: The ABCD3-SLOPE Score.

Background: It is hard to differentiate transient symptoms associated with infarction (TSI) from transient ischemic stroke (TIA) without MRI in the early onset. However, they have distinct clinical outcomes and respond differently to therapeutics. Therefore, we aimed to develop a risk prediction model based on the clinical features to identify TSI.

Aging Induced p53/p21 in Genioglossus Muscle Stem Cells and Enhanced Upper Airway Injury.

Aging of population brings related social problems, such as muscle attenuation and regeneration barriers with increased aging. Muscle repair and regeneration depend on muscle stem cells (MuSCs). Obstructive sleep apnea (OSA) rises in the aging population.

[Cone-beam CT study of the characteristics of midpalatal suture at different age groups in a southern China population].

Purpose: The purpose of this cone-beam CT (CBCT) based study was to investigate the stages of palatal suture at different age groups as well as the bone density of the palatal suture in a south Chinese population.

Methods: The CBCT data of 113 patients with an age range from 4 to 36 years old were selected. All of them were reported to have normal growth.

Effects of the overexpression of IFITM5 and IFITM5 c.-14C>T mutation on human osteosarcoma cells.

The present study aimed to investigate the effects of overexpression of interferon-induced transmembrane protein 5 (IFITM5) and IFITM5 c.-14C>T mutation on osteogenic differentiation, and the proliferation, migration and invasion of SaOS2 cells. SaOS2 cells were transfected with plasmids containing wild type IFITM5 (W) or IFITM5 containing the c.

[A preliminary study on the functional occlusion characters of the patients who had essential root resorption in anterior teeth].

Purpose: In order to determine the effect of functional occlusion on the essential root resorption, we compared the characters of functional occlusion between the patients who had essential root resorption in anterior teeth and who didn't have.

Methods: Panoramic radiography, periapical films and clinical data were used to diagnose the essential root resorption before the patients received orthodontic treatment. Fifteen patients who had 2-3 degree root resorption were divided into the experimental group, while 15 patients who didn't have were chosen as control group according to their age and sex.

Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus.

Background: Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone. However, a major obstacle in the medical application of the HDV ribozyme is the lack of specificity in the delivery of the ribozyme to defined target cells.

Results: The objective of this study was to determine whether retroviral vectors can deliver the HDV ribozyme into the target cells and to elucidate whether HDV ribozyme plays a role in hepatitis B virus (HBV) replication.

Virus-encoded microRNAs: future therapeutic targets?

The discovery of microRNAs (miRNAs) is a remarkable breakthrough in the field of molecular genetics, as miRNAs are key actors which regulate gene expression in diverse cellular processes from unicellular yeast to human. The recent discovery of virus-encoded miRNAs indicates that viruses also use this fundamental mode of gene regulation. Research into viral miRNAs function demonstrates that some miRNAs play an important role in regulating both the viral life cycle and the interaction between viruses and their hosts.

Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA.

Aim: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA.

Methods: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum samples derived from the three different standard curves using certain serum DNA.

[MBT straight wire for Class II division I malocclusion cases].

Objective: To evaluate the therapeutic effects of MBT straight wire on extraction case of Class II division I.

Methods: Thirty-six class II division I malocclusion patients were treated with MBT straight wire by extracting 4 bicuspid premolars or 2 maxillary bicuspid premolars. The X-ray cephalograms were analyzed before and after the treatment of MBT straight wire.