Publications by authors named "Yan-Mei Lian"
Article Synopsis
- A novel mass spectroscopic platform was developed for detecting microRNAs (miRNAs) by integrating ligation reactions, hybridization chain amplification, and multiple-metal-nanoparticle tagging.
- The use of multiple-metal-nanoparticle tagging enhances both specificity and sensitivity, allowing the platform to effectively distinguish between target and non-target miRNAs, even with single-nucleotide differences.
- Achieving limits of detection around 0.5 fM and quantitative results comparable to traditional methods, this platform shows promise for applications in clinical diagnosis and biomedical research.
A facile MALDI-TOF mass spectrometric platform for quantitative analysis of protein biomarkers was developed based on magnetic ZnFeO nanoparticles and mass tagging signal amplification. In this platform, magnetic ZnFeO nanoparticles functionalized with an aptamer of the biomarker of interest was used to magnetically separate silica nanoparticles modified with another aptamer of the target biomarker and a barcoding peptide from solution phase in the presence of the biomarker of interest. After the silica nanoparticles were dissolved by KHF, the released barcoding peptide was detected by MALDI-TOF mass spectrometry with magnetic ZnFeO nanoparticles used as assisting matrix of laser desorption ionization.
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