In vitro evaluation of the impact of a bioceramic root canal sealer on the mechanical properties of tooth roots.

Bacground/purpose: Endodontically treated teeth are more prone to vertical root fracture with the mechanical property changes to some extent during root canal treatment. This study aimed to investigate the effects of a bioceramic sealer on the mechanical properties of tooth roots.

Materials And Methods: Dentin discs were dried by two different methods (ethanol drying and paper points drying) and then filled with a BC sealer named iRoot SP.

Effect of a bioactive glass-based root canal sealer on root fracture resistance ability.

Background/purpose: The root fracture resistance of endodontically treated teeth is decreased significantly, and it is more likely to fracture. This study aimed to evaluate the effect of a novel root canal sealer based on bioactive glass (BG) on root fracture resistance and explore its mechanism.

Materials And Methods: The BG-based root canal sealer (BG Sealer) was prepared by mixing a kind of bioactive glass (10.

A novel bioactive glass-based root canal sealer in endodontics.

Background/purpose: Bioactive glass (BG), one type of bioceramics, shows similar or better characteristics to calcium silicate which has been regarded as a promising root filling material in endodontics. This study aimed to develop a novel BG-based root canal sealer for endodontics.

Materials And Methods: The novel BG-based root canal sealer was composed of phytic acid derived bioactive calcium phosphosilicate glass named PSC mixed with zirconium oxide (ZrO) as powder, and phosphate solution (PS) dissolved with sodium alginate (SA) named PS-SA as liquid.

The effect of topical application of meloxicam on inflamed dental pulp.

Background/purpose: Effective regulation of the inflammatory process is essential for pulp repair and regeneration. Meloxicam has anti-inflammatory activity in systemic administration. The purpose of this study is to observe effects of topically applied meloxicam on inflamed pulp and to explore its potential value in the treatment of pulpitis.

Epigenome-Wide Tobacco-Related Methylation Signature Identification and Their Multilevel Regulatory Network Inference for Lung Adenocarcinoma.

Tobacco exposure is one of the major risks for the initiation and progress of lung cancer. The exact corresponding mechanisms, however, are mainly unknown. Recently, a growing body of evidence has been collected supporting the involvement of DNA methylation in the regulation of gene expression in cancer cells.

Protection of a novel epitope-RNA VLP double-effective VLP vaccine for foot-and-mouth disease.

Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. Previously, we found that the epitope peptide EP displayed on virus-like particles (VLP) for use as a vaccine showed high immunoreactivity and conferred partially effective protection to animals. In this study, we first combined antisense RNA with VLP as a vaccine against the foot-and-mouth disease virus (FMDV) by using a prokaryotic co-expression system.

Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease.

Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work, the coding genes of 141-160 epitope peptide (EP141-160) of VP1 were inserted into the coat protein (CP) genes of MS2 in prokaryotic expression vector, and the recombinant protein self-assembled into virus-like particles (VLP). Results showed that the CP-EP141-160 VLP had a strong immunoreaction with the FMD virus (FMDV) antigen in vitro, and also had an effective immune response in mice.

[Effect of bioactive glasses on angiogenic growth factors of human dental pulp cells].

Objective: To investigate the effects of bioactive glasses (BG) including 45S5 and nano-58S on proliferation, angiogenic markers vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) secretion and gene expression of human dental pulp cells (HDPC).

Methods: HDPC of 4th passage were cultured in Dulbecco's modified Eagle's medium (DMEM) which contained 0.1 g/L 45S5 or nano-58S ionic dissolution products.

[Effects of ionic-dissolution of sol-gel bioactive glasses on human dental pulp cells].

Objective: To evaluate the effects of ionic-dissolution of sol-gel bioactive glasses (BG) on proliferation and alkaline phosphatase (ALP) activity of human dental pulp cells (HDPCs) .

Methods: The primary HDPCs were isolated from intact premolar and third molars and were cultured in DMEM. Then the 4 th generation of HDPCs were cultured in DMEM, which contained 1 g/L of one of 58S, Nano-58S, and 45S5 ionic-dissolution products.

Nano-sized 58S bioactive glass enhances proliferation and osteogenic genes expression of osteoblast-like cells.

Objective: To observe the effects of ionic dissolution products on nano-sized 58S bioactive glass (nano-58S) on proliferation and specific osteogenic genes expression in MG-63 cells.

Methods: Ionic dissolution products were prepared by incubating nano-58S or sol-gel bioactive glass 58S (58S) particulates in Dulbecco's modified Eagle's medium (DMEM) at 1% w/v for 24 hr and filtrated through 0.22 µm filters to remove the particulates.

[Effects of light-curing modes on the polymerization shrinkage and surface hardness of composite resins].

Objective: To investigate the effects of light-curing modes on the polymerization shrinkage and surface hardness of resins and to explore the related clinical relevance.

Methods: Resins with filler content of 76%(mass fraction) were light-cured by high intensity, low intensity and soft start curing modes for 10 s and 20 s, respectively. Specimens for detecting volumetric shrinkage and surface hardness were prepared.

[Evaluation of adaptation of root canal filled with three obturation techniques in vitro].

Objective: To evaluate the adaptation of root canal filled with three obturation techniques in vitro.

Methods: Fifty-seven cleaned and shaped premolars were divided into three groups, each group including 10 single root canal premolars and 9 double root canal premolars, and filled respectively with following techniques: GuttaFlow paste with single master cone (GF group), cold lateral compaction technique with AH plus sealer (LC group), warm vertical compaction technique with AH plus sealer (VC group). The roots were invested and sectioned at 1 mm interval from crown to apex using a microtome saw under water cooling.

[A clinical study on orthodontic treatment after tooth autotransplantation].

Objective: To evaluate the pulp vitality, the periodontal condition and the success rate of autotransplanted teeth after orthodontic treatment.

Methods: A total of 20 permanent teeth (one of them had been treated by root canal therapy), 17 with completely developed roots and 3 with developing roots, were autotransplanted to the region of missing teeth in 16 admitted patients. Orthodontic treatment was carried out in all cases.

Effects of supplementation with multivitamin and mineral on blood pressure and C-reactive protein in obese Chinese women with increased cardiovascular disease risk.

Objective: To investigate the effect of supplementation with multivitamin and mineral on blood pressure and C-reactive protein (CRP) in obese women with increased cardiovascular disease risk as having hypertension, hyperglycemia or hyperlipemia.

Subjects And Methods: 128 obese Chinese women aged 18-55 years with increased cardiovascular disease risk participated in a 26-week randomized, double-blind, placebo-controlled trial. Subjects were randomized to four groups, and received either one tablet of high-dose multivitamin and mineral supplement (MMS), or one tablet of low-dose MMS (Low MMS), or calcium 162 mg (Calcium) or identical placebo (Placebo) daily during the study.

[Effects of maternal high protein diet on uncoupling protein and carnitine palmityl transferase 1 in offspring of rats].

Objective: To investigate the effects of maternal nutritional manipulation on fetal mRNA abundance of uncoupling protein UCP2, UCP3 and carnitine palmityl transferase 1 (CPT1), and find out an optimal maternal diet and targets for pharmacological prevention and treatment of obesity.

Methods: Wistar pregnant rats were assigned to two groups which received a standard diet (SD) and a high protein diet (HPD) during pregnancy, respectively. After delivery, the male offspring were assigned to control group (CON) and high protein group (HP) according to their maternal diet, which were suckled by dams that received SD during pregnancy.

[The anti-respiratory syncytial virus effect of an active compound (AP3) from a Chinese medicinal herb-Herba patriniae in vitro].

Objective: To study the effect on anti-respiratory syncytial virus of an active compound (AP3) from a Chinese medicinal herb-Herba patriniae in vitro.

Methods: Active component of herba patriniae (AP3) was extracted and its anti-respiratory syncytial virus (RSV) effect was tested. A water soluble substance (AP3) was isolated from a Chinese herb Herba patriniae, by hot water extraction, ethol precipitation and gel permeation column chromatography.

[Effect of chewing sugar-free gum after sucrose challenge on dental plaque pH in situ].

Objective: To evaluate the effect of chewing sugar-free gum after sucrose challenge on dental plaque pH in situ.

Methods: 16 healthy volunteers aged 23 - 32 years were screened as subjects. The pH of 48-hour dental plaque was measured using a Beetrode pH microelectrode when subjects chewed Extra sugar-free gum after sucrose challenge.

Plaque minerals in the prediction of caries activity.

Background/aims: Attempts to identify subjects who will develop caries lesions in future years have been only moderately successful, suggesting that one or more important risk factors are being overlooked. The aim of this study was to validate the use of plaque calcium, phosphate and fluoride concentrations as significant risk factors in caries.

Methods: DMFS and DMFT rates were measured three times over 2 years in a group of rural Chinese schoolchildren initially aged 12 years and not living in a high-fluoride area.