Comprehensive next-generation sequencing reveals double primary colorectal carcinoma missed by diagnostic imaging: A case report.

Background: Multiple primary colorectal carcinoma (MPCC) is a rare clinical disease, which is challenging to differentiate from metastatic disease using histopathological methods. Next-generation sequencing (NGS) has been employed to identify multiple primary cancers.

Case Summary: This study a rare case of a 63-year-old male patient diagnosed with MPCC by targeted NGS, which was initially missed by radiological evaluation.

Biosynthesis and evaluation of a novel highly water-soluble quercetin glycoside derivative.

Quercetin () was converted into quercetin 7--succinyl glucoside () by used FJ18 as a solvent-resistant whole-cell biocatalyst. The structure of the new compound was confirmed by LC-MS analysis and NMR spectroscopy. The water-solubility of this novel quercetin 7--succinyl glucoside () was approximately 1000 times higher than that of native quercetin ().

Design of an adjustable bipod flexure for a large-aperture mirror of a space camera.

An adjustable bipod flexure (ABF) technique for a large-aperture mirror of a space camera is presented. The proposed flexure mount can decrease the surface distortions caused by the machining error and the assembly error of the mirror assembly (MA) in a horizontal optical testing layout. Through the analysis of the compliance matrix of conventional bipod flexure, the positional relationship between the rotation center and the apex of the flexure is investigated.

Endoplasmic reticulum stress regulates proliferation, migration and invasion of human ovarian cancer SKOV3 cells through PI3K/AKT/mTOR signaling pathway.

Objective: The study is to explore the role of tunicamycin-induced endoplasmic reticulum stress (ERS) in human ovarian cancer (OC) SKOV3 cells proliferation, migration and invasion by modulating the activity of PI3K/AKT/mTOR pathway.

Methods: The collected human OC SKOV3 cells were randomly separated into three groups: The control group, the Tun group (treated with tunicamycin to induce ERS) and the CHOP-si group (transfected with CHOP-siRNA before tunicamycin treatment). CCK-8 method was applied for testing cell proliferation, while flow cytometry was conducted to detect cell apoptosis.