Inflammation- and tumor-induced anorexia and weight loss require MyD88 in hematopoietic/myeloid cells but not in brain endothelial or neural cells.

Loss of appetite is a hallmark of inflammatory diseases. The underlying mechanisms remain undefined, but it is known that myeloid differentiation primary response gene 88 (MyD88), an adaptor protein critical for Toll-like and IL-1 receptor family signaling, is involved. Here we addressed the question of determining in which cells the MyD88 signaling that results in anorexia development occurs by using chimeric mice and animals with cell-specific deletions.

[Bone marrow stromal cells co-modified with BMP-2 and bFGF gene].

Aim: To establish modified bone marrow stromal cells(BMSCs) which can express BMP-2 and bFGF stably.

Methods: BMP-2 and bFGF gene were amplified by RT-PCR, and then cloned into the expression vector pcDNA3.0.