[Intestinal dysfunction of spinal cord injury treated with moxibustion: a randomized controlled trial].

Objective: To compare the clinical effect on intestinal dysfunction of spinal cord injury (SCI) between the comprehensive therapy of moxibustion (moxibustion for opening the governor vessel and regulating the spirit) and rehabilitation training and the simple treatment with rehabilitation training.

Methods: A total of 60 patients with intestinal dysfunction of SCI were randomized into a comprehensive therapy group and a rehabilitation group, 30 cases in each one (3 cases were dropped out in each group). On the base of the routine western medicine treatment and rehabilitation training, the bowel training and rectal function training were provided, once a day in the rehabilitation group.

[Potential Value of Cancer-Associated Fibroblasts in Hematological Malignancies--Review].

Cancer-associated fibroblasts (CAF), as one of the most important components of tumor microenvironment, which plays important role in tumorigenesis, development, infiltration and metastasis of cancers. In a variety of solid tumors, CAF can even determine the fate of tumor cells. In view of its pivotal role in promoting tumor progression, CAF has recently become a therapeutic target for a variety of tumors.

Knockdown of AGR2 induces cell apoptosis and reduces chemotherapy resistance of pancreatic cancer cells with the involvement of ERK/AKT axis.

Background: Pancreatic cancer (PC), an aggressive human malignancy, presents with a striking resistance to chemotherapy. Interesting, AGR2 has been found to be upregulated in various cancers and has been found to promote the dissemination of PC cells. Thereby, a series of in-vitro experiments were performed to investigate the relationship between AGR2 and the ERK/AKT axis, and to explore whether it affects PC cells.

Lentiviral-mediated short hairpin RNA silencing of APE1 suppresses hepatocellular carcinoma proliferation and migration: A potential therapeutic target for hepatoma treatment.

Apurinic/apyrimidinic endonuclease-1 (APE1) is a protein involved in DNA repair and transcriptional regulation of gene expression. APE1 expression was reported to be correlated with poor prognosis in hepatocellular carcinoma (HCC) patients. Based on our previous study, we hypothesized that APE1 may be involved in the metastatic progression of HCC.

Effect of serum choice on replicative senescence in mesenchymal stromal cells.

Background Aims: Multipotent mesenchymal stromal cells (MSCs) are promising candidates for innovative cell therapeutic applications. Before their use, however, they usually need to be expanded in vitro with serum-supplemented media. MSCs can undergo replicative senescence during in vitro expansion, but it is not yet clear how serum supplements influence this process.

Rutaecarpine inhibits angiotensin II-induced proliferation in rat vascular smooth muscle cells.

Objective: To evaluate the effects and possible mechanisms of rutaecarpine on angiotensin II (Ang II)-induced proliferation in cultured rat vascular smooth muscle cells (VSMCs).

Methods: VSMCs were isolated from Male Sprague-Dawley rat aorta, and cultured by enzymic dispersion method. Experiments were performed with cells from passages 3-8.

Evaluation of vaporized hydrogen peroxide fumigation as a method for the bio-decontamination of the high efficiency particulate air filter unit.

Objective: To evaluate the performance of vaporized hydrogen peroxide (VHP) for the bio-decontamination of the high efficiency particulate air (HEPA) filter unit.

Methods: Self-made or commercially available bioindicators were placed at designated locations in the HEPA filter unit under VHP fumigation. The spores on coupons were then extracted by 0.

Decontamination of Bacillus subtilis var. niger spores on selected surfaces by chlorine dioxide gas.

Objective: Chlorine dioxide (CD) gas has been used as a fumigant in the disinfection of biosafety laboratories. In this study, some experiments were conducted to assess the inactivation of spores inoculated on six materials [stainless steel (SS), painted steel (PS), polyvinyl chlorid (PVC), polyurethane (PU), glass (GS), and cotton cloth (CC)] by CD gas. The main aims of the study were to determine the sporicidal efficacy of CD gas and the effect of prehumidification before decontamination on sporicidal efficacy.

[The effects of mesenchymal stem cells on the aging kidneys in rats].

Objective: To study the effect of mesenchymal stem cells on the aging rat kidney and to explore the underlying mechanism.

Methods: Rat models of senile kidney were built with hypodermic injection of D-galactose daily. Injections of MSCs of 3 x 10(6) were given to each rat through vena caudalis and CFSE was used as a tracing label to detect the distribution of MSCs in vivo.

[In vitro tracing of rat bone marrow mesenchymal stem cells using the fluorescent dye CFSE].

Objective: To determine the optimal condition for labeling rat mesenchymal stem cells (MSCs) using the fluorescent dye CFSE and the maximum time length allowed by CFSE staining for MSC tracing in vitro.

Methods: Rat MSCs were labeled with CFSE at different concentrations (2.5, 5.

[Different cytotoxicity of NK cells against high and low expression of ATP-binding cassette transporter (ABCG(2)) of human multi-drug resistant nasopharyngeal carcinoma cells].

Aim: To investigate the different cytotoxicity sensitivity of high and low expression of ATP-binding cassette transporter (ABCG(2)) of human multi-drug resistant nasopharyngeal carcinoma CNE2/DDP cell line (ABCG(2)(High) cell and ABCG(2)(Low cell)) to NK cells.

Methods: ABCG(2)(High)CNE2/DDP cells, ABCG(2)(Low) CNE2/DDP cells and NK cells were isolated by magnetic activated cell sorting (MACS). The cytotoxic effects of NK cells against K562, ABCG(2)(High) and ABCG(2)(Low)CNE2/DDP cells were detected with LDH releasing assay.

[Application of fingerprint of active part on extraction techniques for Vernonia anthelmintica].

Objective: To probe into feasibility of extraction techniques for Vernonia anthelmintica using HPLC fingerprint of active part as model.

Methods: The extraction techniques were studied by multi-index test experiment formula evaluation and orthogonal design, which took change of peak area in HPLC fingerprint of active part for Vernonia anthelmintica as indices.

Results: The optimum extracting procedure was as follows: using 10 times volume of water for each time, soaking one hour and then boiling for three times sustaining one hour each.

[Create mathematical model and analysis of correlation between Chinese medicinal characteristics and immunoregulatory activity based on literature informatics].

Objective: Intends to create mathematical model and analysis of correlation between Chinese medicinal characteristics and immunoregulatory activity based on literature informatics.

Method: The numbers of the Chinese medicines with immune effects were worked out within the framework of "The China Pharmacopeia" of 2005 edition, from the literature publicized since 1980. The correlation and mathematical model were figured out between Chinese medicinal characteristics including biological classification, different tastes, channel tropism as well as the parts used and immunoregulatory activity based on the statistical software SPSS.