Publications by authors named "Yan-Hui Zheng"
Article Synopsis
- A new method for detecting berberine in Rhizoma coptidis using β-cyclodextrin-sensitized fluorescence technology has been developed, allowing for sensitive measurement of this medicinal compound.
- The method utilizes specific wavelengths (excitation at 345 nm and emission at 540 nm) to avoid interference and demonstrate a low limit of detection alongside a broad linear range of concentrations.
- Testing revealed that berberine constitutes 7.60% of the Rhizoma coptidis sample, with a recovery rate of 99.5%, and results were consistent with another established detection method, indicating its reliability and practicality for medicinal applications.
Objective: To investigate the expression and correlation of hypoxia-inducible factor-1alpha (HIF-1alpha), vascular endothelial growth factor (VEGF) and sFlt-1 in the preeclampsia placenta, and discuss their significance in the pathogenesis of preeclampsia.
Methods: Placentas were collected from 20 pregnant women with preeclampsia as study group and 15 normal pregnant women as control group. The expressions of HIF-1alpha, VEGF and sFlt-1 protein were semi-quantitatively analyzed with immunohistochemical assay and mRNA level was determined using reverse transcription polymerase chain reaction (RT-PCR) technique.