Effects of brief mindfulness on anxiety-provoked adverse events during ultrasound-guided fine-needle aspiration biopsy of thyroid nodules.

Purpose: This study aims to test the effectiveness of brief mindfulness for anxiety-provoked adverse events during ultrasound-guided fine needle aspiration biopsy (FNAB).

Results: A total of 439 patients were randomized to two groups. The ratio of anxiety in all patients with TN before FNAB was 25.

[Application of miR-182 for Determination of Glucocorticoid-Resistant Patients with Lymphoid Malignancy].

Objective: To validate the clinical relationship between miR-182 and glucocorticoid-resistance in patients with lymphoid malignancy.

Methods: Real-time quantitative PCR(qRT-PCR) was employed to detect the expression of miR-182 in lymphoma patients (68 cases, the specimens indluded bone marrow of 20 cases, and plasma of 48 cases), multiple myeloma patients (24 cases, the specimens included bone marrow of 14 cases and plasma of 10 cases), ALL patients (3 cases, specimen was plasma of 3 cases) and non-lymphotic system disorder patients (18 cases, specimens included bone marrow of 8 cases and plasma of 10 cases).

Results: The expression of miR-182 in refractory lymphoblastic tumor patients was significantly higher than that in initial treatment group (P<0.

[Change and significance of Th17 cells and its secretive IL-17 in patients with autoimmune hemolytic anemia].

Aim: To observe the percentage of Th17 cells in the peripheral blood and levels of plasma IL-17 of patients with autoimmune hemolytic anemia (AIHA)before treatment and after glucocorticoid treatment and to explore their clinical significance.

Methods: Flow cytometric assay were used to detect the rate of Th17 cells in AIHA patients. The level of plasma IL-17 in AIHA patients were measured by ELISA.

[Amaxa Nucleofector(TM) nuclear transfection apparatus transfers L1210 cell line].

Mouse L1210 leukemia cell line is widely used as a model in the study of tumorigenesis, as well as the efficacy of chemotherapeutic drugs; however, like other suspension cell lines, the mouse L1210 cell line has lowest transfection efficiency, that many barriers exist to study about the structure, function, as well as metabolism in leukemia cells. This study was aimed to obtain higher transfection efficiency of L1210 cell line to facilitate scientific research. The transfection efficiencies of nucleofector and liposome in L1210 leukemia cells were detected by converted fluorescence microscopy and flow cytometry using EGFP (enhance green fluorescent protein); cell viability was observed by trypan blue exclusion test.

[Combination of dexamethasone with IL-2 selectively induces the expansion of CD4(+)CD25(+)FOXP3(+) regulatory T cells in vivo and suppresses graft versus host disease.].

Objective: To establish a method for increasing T regulatory cells (Treg) in the graft by using in vivo treatment with dexamethasone (Dex) plus IL-2 and observe its suppressing effect on graft-versus-host-disease (GVHD) in mice.

Methods: After treatment of male C57BL/6N mice (donor) with Dex (5 mgxkg(-1)xd(-1)) combined with IL-2 (300 000 IUxmouse(-1)xday(-1)) for three days, spleen mononuclear cells were isolated for flow cytometry analysis of CD4(+)CD25(+) POXP3(+) Treg cells. The allogeneic lymphocytes were transplanted from male C57BL/6N mice to female BALB/c mice aged 8-12 weeks.

[Mechanism underlying IL-2 inhibition on T cell specific immune response].

The study was aimed to investigate the effect of IL-2 on the acquired immune response of naive T cells, and to explore the influence of IL-2 on suppressor of cytokine signaling 3 (SOCS-3) expression of naive T cells, as well as to elucidate the role of SOCS-3 on antigen specific immune response in vitro. Naive DO11.10 T cells were pre-stimulated with IL-2 (50 U/ml), and stimulated with OVA(323 - 329) antigen after removing IL-2, then the proliferation of naive DO11.

[Effect of HMBA on differentiation and apoptosis of HL-60 and U937 cells and its mechanism].

Objectives: To explore the effect of hexamethylene bisacetamide (HMBA) on the differentiation and apoptosis of HL-60 and U937 cells, and its mechanism.

Methods: Flow cytometry was used to evaluate the expressions of cellular surface antigen CD(11b), CD(14), apoptotic marker Annexin V, cell cycle distribution and endocytic antigen cyclin D, cyclin E and p27. Changes of c-myc, Rb, Bcl-2 gene mRNA levels were detected by RT-PCR.

Clinical and laboratory studies of expression of cyclin A in leukemia cells.

Uncontrolled cell proliferation is the basic feature of cancer. Some of the prime cell cycle regulators are involved directly in tumorigenesis. Cyclin A, one of the G(1)/S cyclin, can cause transformation.

[Proliferation and IFN-gamma secretion of autologous T lymphocytes stimulated by myeloid leukemia cells induced with rhGM-CSF and rhIL-4].

To observe the proliferation of T lymphocytes stimulated by CML and AML cells which were induced by rhGM-CSF and rhIL-4, and the secretion of IFN-gamma from proliferated T lymphocytes, the expression of CD80, CD86 and HLA-DR on CML and AML cells induced by GM-CSF and IL-4 was assayed by flow cytometry in vitro. Then one-way mixed lymphocyte reaction was carried out, with induced leukemia cells as stimulating cells and auto-T lymphocytes as reactive cells. The secretion of IFN-gamma from T lymphocytes was determined by double antibody sandwich ELISA.