POU2F2-mediated upregulation of lncRNA inhibits ferroptosis in breast cancer via miR-376c-3p/SLC7A11 axis.

Triple-negative breast cancer (TNBC) is a subtype of BC with high rates of mortality. The mechanism of in ferroptosis of TNBC was investigated. Chromatin immunoprecipitation and dual-luciferase reporter assays were used to measure intermolecular relationships.

[Construction and identification of shRNA expressing vectors targeting RhoA gene].

Aim: To construct and identify shRNA interference vector targeting RhoA gene.

Methods: Two pairs of single stranded oligonucleotides encoding RhoA siRNA sequence were designed and synthesized. After annealing, its were inserted into vector pGPU6/GFP/Neo, constructed recombinant vectors and then were identified by restrictive digestion and DNA sequencing.