[Expression and clinical significance of CD3, CD4 and COX-2 in non-small cell lung cancer].

Aim: To study expression and clinical significance of CD3, CD4 and COX-2 in non-small cell lung cancer (NSCLC).

Methods: Expression of COX-2, CD3 and CD4 was detected by immunohistochemical staining in 37 cases of NSCLC. The correlation of CD3, CD4, COX-2 expressions and overall survival(OS) was evaluated with spearman rank correlation analysis.

Antigenically dominant proteins within the human liver mitochondrial proteome identified by monoclonal antibodies.

Analysis of the mitochondrial proteome would provide valuable insight into the function of this important organelle, which plays key roles in energy metabolism, apoptosis, free radical production, thermogenesis, and calcium signaling. It could also increase our understanding about the mechanisms that promote mitochondrial disease. To identify proteins that are antigenically dominant in human liver mitochondria, we generated >240 hybridoma cell lines from native mitochondrial proteins after cell fusion, screening, and cloning.

[Preparation and identification of monoclonal antibody against enoyl-CoA hydratase 1].

Objective: To prepare monoclonal antibodies (mAbs) against enoyl-CoA hydratase 1 (ECH1).

Methods: Normal human liver tissues were homogenized, and the mitochondria were isolated by differential centrifugation. The total mitochondrial proteins were used to immunize BALB/c mice to prepare mAbs by routine hybridoma technique.

[Preparation, characterization and application of monoclonal antibody against CPS1].

Aim: To prepare and characterize the monoclonal antibody (mAb) against human carbamyl phosphate synthetase I (CPSI) and make a study of its application.

Methods: Normal human liver tissues were homogenized, and their mitochondria were isolated by differential centrifugation. The total mitochondrial proteins were used to immunize BALB/c mice to prepare mAb using the routine hybridoma technique.

[Preparation and characterization of monoclonal antibody against DCXR].

Aim: To prepare monoclonal antibodies (mAbs) against Dicarbonyl/L-xylulose reductase (DCXR).

Methods: Normal human liver tissues were homogenized, and mitochondria were isolated by differential centrifugation. The total mitochondrial proteins were used to immunize BALB/c mice to prepare mAbs by routine hybridoma technique.