Antibacterial mechanism of high-mobility group nucleosomal-binding domain 2 on the Gram-negative bacteria Escherichia coli.

Objective: To investigate the antibacterial mechanism of high-mobility group nucleosomal-binding domain 2 (HMGN2) on Escherichia coli K12, focusing on the antibacterial and antibiofilm formation effects. Its chemotactic activity on human neutrophils was also investigated.

Methods: Human tissue-derived HMGN2 (tHMGN2) was extracted from fresh uterus fiber cystadenoma and purified by HP1100 reversed-phase high-performance liquid chromatography (RP-HPLC).

Multiple scattering of electromagnetic waves by an aggregate of uniaxial anisotropic spheres.

An exact analytical solution is obtained for the scattering of electromagnetic waves from a plane wave with arbitrary directions of propagation and polarization by an aggregate of interacting homogeneous uniaxial anisotropic spheres with parallel primary optical axes. The expansion coefficients of a plane wave with arbitrary directions of propagation and polarization, for both TM and TE modes, are derived in terms of spherical vector wave functions. The effects of the incident angle α and the polarization angle β on the radar cross sections (RCSs) of several types of collective uniaxial anisotropic spheres are numerically analyzed in detail.

[Study on antimicrobial activity of human hemoglobin and its fragments both in vitro and in vivo].

Objective: To isolate human hemoglobin and its fragrments, compare their antimicrobial activity in vitro and pilot study of their antimicrobial activity in vivo.

Methods: The alpha and beta chains of hemoglobin were separated by cation exchange chromatography and gel chromatography; The alpha and beta chains were cleaved by cyanogens bromide respectively. The cleaved fragments were purified by reverse phase high performance liquid chromatography.