A simple and sensitive test for colonization, surveillance, and infection control suitable for near patient use.

Unlabelled: is a multidrug-resistant fungal pathogen with a propensity to colonize humans and persist on environmental surfaces. invasive fungal disease is being increasingly identified in acute and long-term care settings. We have developed a prototype cartridge-based surveillance assay (CaurisSurV cartridge; "research use only") that includes integrated sample processing and nucleic acid amplification to detect from surveillance skin swabs in the GeneXpert instrument and is designed for point-of-care use.

Clinical Course, Antifungal Susceptibility, and Genomic Sequencing of Trichophyton indotineae.

Importance: Trichophyton indotineae is an emerging dermatophyte causing outbreaks of extensive tinea infections often unresponsive to terbinafine. This species has been detected worldwide and in multiple US states, yet detailed US data on infections with T indotineae are sparse and could improve treatment practices and medical understanding of transmission.

Objective: To correlate clinical features of T indotineae infections with in vitro antifungal susceptibility testing results, squalene epoxidase gene sequence variations, and isolate relatedness using whole-genome sequencing.

PERSISTENT SUBRETINAL FLUID AFTER VITRECTOMY FOR MACULAR HOLE-ASSOCIATED RETINAL DETACHMENT.

Purpose: To evaluate the incidence, associated factors, and outcome of persistent subretinal fluid (SRF) after vitrectomy for macular hole-associated retinal detachment (MHRD).

Methods: A total of 158 eyes from 156 patients with MHRD who achieved macular hole closure after primary vitrectomy were included in the analysis; persistent SRF was defined as the presence of SRF for more than 1 month after first surgery. Preoperative and postoperative parameters were analyzed for their relationship with SRF development.

Development and Validation of TaqMan Chemistry Probe-Based Rapid Assay for the Detection of Echinocandin-Resistance in Candida auris.

Candida auris is a multidrug-resistant yeast pathogen causing outbreaks in health care facilities worldwide, and the emergence of echinocandin-resistant C. auris is a concern. Currently used Clinical and Laboratory Standards Institute (CLSI) and commercial antifungal susceptibility tests (AFST) are phenotype-based, slow, and not scalable, limiting their effectiveness in the surveillance of echinocandin-resistant C.

Candida auris admission screening pilot in select units of New York City health care facilities, 2017-2019.

Background: This pilot project implemented admission screening for Candida auris (C. auris) using real-time polymerase chain reaction (rt-PCR) in select high-risk units within health care facilities in New York City.

Methods: An admission screening encounter consisted of collecting 2 swabs, to be tested by rt-PCR, and a data collection form for individuals admitted to ventilator units at 2 nursing homes (NHA and NHB), and the ventilator/pulmonary unit, intensive care unit, and cardiac care unit at a hospital (Hospital C) located in New York City from November 2017 to November 2019.

Utility of CHROMagar™ Candida Plus for presumptive identification of Candida auris from surveillance samples.

Candida auris is a nosocomial fungal pathogen of prime importance due to its global emergence and rapid spread in healthcare facilities worldwide. One important concern is that routine, conventional methods fail to identify C. auris.

Calcimycin Inhibits and by Targeting the Prp8 Intein Splicing.

Drug resistance is a significant concern in the treatment of diseases, including cryptococcosis caused by () and (). Alternative drug targets are necessary to overcome drug resistance before it attains a critical stage. Splicing of inteins from pro-protein precursors is crucial for activities of essential proteins hosting intein elements in many organisms, including human pathogens such as and .

Candida auris Pan-Drug-Resistant to Four Classes of Antifungal Agents.

Candida auris is an urgent antimicrobial resistance threat due to its global emergence, high mortality, and persistent transmissions. Nearly half of C. auris clinical and surveillance cases in the United States are from the New York and New Jersey Metropolitan area.

A description of the first Candida auris-colonized individuals in New York State, 2016-2017.

Candida auris (C. auris) is a globally emerging multidrug-resistant yeast. New York State (NYS) first detected C.

Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019).

Blastomycosis due to and is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of and The new assay permitted retrospective analysis of cultures (2005 to 2019) and primary clinical specimens from blastomycosis cases (2013 to 2019) from New York patients. We identified as the predominant pathogen in 38 cases of blastomycosis, while was a minor pathogen involved in five cases; these findings expand understanding of blastomycosis in New York.

Activity of Manogepix against Multidrug-Resistant and Panresistant Candida auris from the New York Outbreak.

An ongoing outbreak in the New York metropolitan area is the largest recorded to date in North America. Laboratory surveillance revealed NY isolates are resistant to fluconazole, with variable resistance to other currently used broad-spectrum antifungal drugs, and that several isolates are panresistant. Thus, there is an urgent need for new drugs with a novel mechanism of action to combat the resistance challenge.

Laboratory Analysis of an Outbreak of Candida auris in New York from 2016 to 2018: Impact and Lessons Learned.

is a multidrug-resistant yeast which has emerged in health care facilities worldwide; however, little is known about identification methods, patient colonization, environmental survival, spread, and drug resistance. Colonization on both biotic (patients) and abiotic (health care objects) surfaces, along with travel, appear to be the major factors for the spread of this pathogen across the globe. In this investigation, we present laboratory findings from an ongoing outbreak in New York (NY) from August 2016 through 2018.

Magnetic resonance imaging of the zone of calcified cartilage in the knee joint using 3-dimensional ultrashort echo time cones sequences.

Background: The zone of calcified cartilage (ZCC) plays an important role in the pathogenesis of osteoarthritis (OA) but has never been imaged in vivo with magnetic resonance (MR) imaging techniques. We investigated the feasibility of direct imaging of the ZCC in both cadaveric whole knee specimens and in vivo healthy knees using a 3-dimensional ultrashort echo time cones (3D UTE-Cones) sequence on a clinical 3T scanner.

Methods: In all, 12 cadaveric knee joints and 10 in vivo healthy were collected.

Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus.

We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing results for the prediction of inducible clarithromycin drug resistance.

Preparation and structural characterization of a series of monoacylhydrazidate-bridged coordination polymers.

From the simple hydrothermal reactions of metal salts, organodicarboxylic acids and hydrazine hydrate sometimes in the presence of 1,10-phenanthroline (phen), a series of monoacylhydrazidate-containing complexes including three Pb(II) coordination polymers as 1-D chained [Pb(2)(PTH)(4)(phen)(2)].H(2)O (PTH = phthalhydrazidate) , 1-D ribboned [Pb(2,3-PDH)(2)] (2,3-PDH = pyridine-2,3-dicarboxylhydrazidate) and 3-D grid-like [Pb(3,4-PDH)(2)] (3,4-PDH = pyridine-3,4-dicarboxylhydrazidate) as well as several transition metal complexes as 2-D layered [Cu(PTH)] , mononuclear [M(2,3-PDH)(2)(H(2)O)(2)] (M = Mn , Co and Zn ) and mononuclear [Mn(H(2)O(6))] (PTH)(2).4H(2)O were obtained.