Identification of the saccharifying microbiota based on the absolute quantitative analysis in the batch solid-state fermentation system.

The fermentation process of Chinese baijiu, a distinctive example of batch solid-state fermentation (BSSF), involves the recurrent use of the same raw material to optimize starch utilization. However, it is unclear which microorganisms are able to metabolize low concentration starch effectively. In this study, we successfully identified the key saccharifying microbiota that degraded low-concentration starch in the BSSF system by absolute quantification techniques.

Rates and Factors Related to COVID-19 Vaccine Uptake in Racialized and Indigenous Individuals in Canada: The Deleterious Effect of Experience of Racial Discrimination.

Racialized and Indigenous communities have been disproportionately affected by COVID-19 infections and mortality, driven by systemic socioeconomic inequalities. However, how these factors specifically influence COVID-19 vaccine uptake is not documented among racialized individuals in Canada. The present study aims to examine COVID-19 vaccine uptake rates and related factors among racialized and Indigenous communities compared to White people in Canada.

SLC25A21 correlates with the prognosis of adult acute myeloid leukemia through inhibiting the growth of leukemia cells via downregulating CXCL8.

In recent years, targeting mitochondrial apoptosis has emerged as a promising therapeutic strategy for Acute Myeloid Leukemia (AML). The SLC25 family of mitochondrial carriers plays a critical role in maintaining mitochondrial function and regulating apoptosis. However, the role of SLC25A21, an oxodicarboxylate carrier, in AML progression and its potential as a prognostic biomarker remain underexplored.

YAP/TAZ mediates resistance to KRAS inhibitors through inhibiting proapoptosis and activating the SLC7A5/mTOR axis.

KRAS mutations are frequent in various human cancers. The development of selective inhibitors targeting KRAS mutations has opened a new era for targeted therapy. However, intrinsic and acquired resistance to these inhibitors remains a major challenge.

Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization.

Organic fertilizer application promotes the prevalence of antibiotic resistance genes (ARGs), yet the factors driving temporal differences in ARG abundance under long-term organic fertilizer application remain unclear. This study investigated the temporal dynamics of ARG diversity and abundance in both bulk and rhizosphere soils over 17 years (2003-2019), and explored microbial evolution strategies, ARG hosts succession and the influence of root exudates on ARGs regulation. The results showed that the ARGs abundance in rhizosphere soil was lower than that in bulk soil under long-term fertilization, and ARGs abundance exhibited a decrease and then remained stable in rhizosphere soil over time.

Analysis of contemporary value and influence of intangible cultural heritage based on online review mining.

Purpose: The development of new media has enabled intangible cultural heritage to be disseminated through online platforms and attracted the attention of many contemporary young people. Classification and discussion on the value of intangible cultural heritage is an important way to help the inheritance and dissemination.

Design/methodology/approach: Real online reviews were collected based on the Bilibili website as the research data source.

Progressive decrease in partial pressure of end-tidal CO during posterior spinal fusion surgery in a child with idiopathic scoliosis and pectus excavatum: a case report.

Background: Scoliosis surgery performed in a prone position may result in thoracic anatomical compression and alter local hemodynamics, increasing surgical risk, especially in patients with pectus excavatum. Most commonly, refractory hypotension is the first symptom of these circulatory changes. Here, we report a case with scoliosis and pectus excavatum under posterior spinal fusion that presented as a progressive decrease in the partial pressure of end-tidal CO (PCO) as the first symptom in the prone position.

Factors influencing the time to COVID-19 antigen tests negative conversion among students at a Chinese university: a retrospective analysis.

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause repeated infections. China experienced an outbreak of COVID-19 (coronavirus disease 2019) after the Chinese government changed its COVID-19 policy in December 2022. However, little is known about the influencing factors, such as a history of previous infection and vaccine dose, and whether these factors affect the time at which the antigen test results become negative for SARS-CoV-2.

Long-term impact of maternal pre-pregnancy BMI on the risk of overweight/obesity in preschool children: mediation by infant birth weight and BMI trajectory group from 0 to 2 years.

Background: Obesity is a complex multisystem disease that affects almost every organ system. Studying the early determinants of obesity and implementing targeted interventions have positive implications for promoting long-term health. Therefore, this study was to evaluate the impact of maternal pre-pregnancy overweight or obesity, weight gain rate (WGR) during the second and third trimesters, infant birth weight (IBW) and BMI trajectory group (BMI-TG) from 0 to 2 years on overweight or obesity in preschool children.

Postoperative femoral head cartilage injury after hip arthroscopic treatment for femoroacetabular impingement syndrome and labral tear.

Background: Postoperative femoral head cartilage injury (FHCI) is a rare condition that can be observed in a certain proportion of patients undergoing hip arthroscopy. However, the prevalence and associated factors of FHCI, and the effect of this condition on clinical outcomes still remain unknown.

Patients And Methods: Consecutive patients who were diagnosed with femoroacetabular impingement syndrome (FAIS) and labral tear and underwent hip arthroscopic treatment in our institute between July 2020 and July 2021 were retrospectively evaluated.

Clinical Insights and Management Strategies for Acute Obstructive Suppurative Pancreatic Ductitis: A Retrospective Study of 82 Rare Cases.

Background And Aims: Acute obstructive suppurative pancreatic ductitis (AOSPD) is a rare condition with limited diagnostic and treatment information. This study aims to expand understanding by analyzing the clinical characteristics and management of AOSPD.

Methods: We analyzed 81 AOSPD cases (1993-2024) from the literature and 1 case from the First Affiliated Hospital of Anhui Medical University.

Glycyrol targets Pneumolysin (PLY) oligomerization to reduce toxicity.

Aim Of The Study: Exploring the potential of glycyrol to reduce the invasiveness of ().

Materials And Methods: Cell experiments were performed using A549 alveolar epithelial cells and D39. Glycyrol was added to A549 cells mixed with or without Pneumolysin (PLY) to detect the effect of Glycyrol on PLY toxicity.

A DNA tweezer-actuated nanozyme-enzyme hybrid nanoreactor for pesticide detection.

The construction of a nanozyme-enzyme hybrid cascade system is an effective protocol to optimize the performance of biosensors. Yet, the integration has limitations due to the lack of harmonious collaboration between nanozyme and enzyme. Herein, we have constructed an efficient enzymatic cascade system by utilizing the base complementary pairing and the targeting capability of DNA tweezers to combine DNA-regulated copper nanoflowers (CuNFs) with acetylcholinesterase (AChE).

Potential Protective Effects of Pungent Flavor Components in Neurodegenerative Diseases.

Neurodegenerative diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD) have become a major global health burden, but the detailed pathogeneses of neurodegenerative diseases are still unknown, and current treatments are mainly aimed at controlling symptoms; there are no curative treatments for neurodegenerative diseases or treatments for the progressive cognitive, behavioral, and functional impairments that they cause. Studies have shown that some plant extracts with pungent flavor components have a certain neuroprotective effect in neurodegenerative diseases, and their mechanisms mainly involve inhibiting neuronal apoptosis, promoting neuronal regeneration, reducing mitochondrial degeneration, and reducing the production of oxides such as reactive oxygen species in cells, which are of great significance for exploring the treatment of neurodegenerative diseases. In this review, we searched the PubMed database for relevant literature collected in the past 15 years.

New Insight into the Related Candidate Genes and Molecular Regulatory Mechanisms of Waterlogging Tolerance in Tree Peony .

Research on the waterlogging tolerance mechanisms of helps us to further understand these mechanisms in the root system and enhance its root bark and oil yields in southern China. In this study, root morphological identification, the statistics of nine physiological and biochemical indicators, and a comparative transcriptome analysis were used to investigate the waterlogging tolerance mechanism in this plant. As flooding continued, the roots' vigor dramatically declined from 6 to 168 h of waterlogging, the root number was extremely reduced by up to 95%, and the number of roots was not restored after 96 h of recovery.

Use of Transcriptomics to Identify Candidate Genes for Hematopoietic Differences Between Wujin and Duroc Pigs.

Hematopoiesis is a complex physiological process that ensures renewal of blood cells to maintain normal blood circulation and immune function. Wujin pigs exhibit distinct characteristics such as tender meat, high fat storage, strong resistance to roughage, robust disease resistance, and oxidation resistance. Therefore, using Wujin pigs as models may offer valuable insights for hematopoietic-related studies.

First-line treatments for KRAS-mutant non-small cell lung cancer: current state and future perspectives.

mutations are common in non-small cell lung cancer (NSCLC) and are associated with patient prognosis; however, targeting has faced various difficulties. Currently, immunotherapy, chemotherapy, and chemoimmunotherapy play pivotal roles in the first-line treatment of -mutated NSCLC. Here, we summarize the current evidence on first-line therapies and compare the treatment outcomes and biomarkers for different regimens.

scPAS: single-cell phenotype-associated subpopulation identifier.

Despite significant advancements in single-cell sequencing analysis for characterizing tissue sample heterogeneity, identifying the associations between cell subpopulations and disease phenotypes remains a challenging task. Here, we introduce scPAS, a new bioinformatics tool designed to integrate bulk data to identify phenotype-associated cell subpopulations within single-cell data. scPAS employs a network-regularized sparse regression model to quantify the association between each cell in single-cell data and a phenotype.

Exploring the predictors of foreign language anxiety: the roles of language proficiency, language exposure, and cognitive control.

This research delves into unexplored territories of Foreign Language Anxiety (FLA), going beyond the traditional focus on language proficiency. We examined the nuanced roles of language exposure and individual differences in cognitive control abilities in shaping FLA. By engaging 46 English learners in a comprehensive assessment, our analysis uncovered significant yet distinct contributions of these factors to various aspects of FLA.

Lower respiratory tract microbiome is associated with checkpoint inhibitor pneumonitis in lung cancer patients.

Background: The gut microbiome is associated with the occurrence and severity of immune-related adverse events (irAEs) in cancer patients undergoing immunotherapy. However, the relationship between the lower respiratory tract (LRT) microbiome and checkpoint inhibitor pneumonitis (CIP) in lung cancer patients who underwent immunotherapy is unclear. The aim of the present study was to investigate the associations between the LRT microbiome and CIP in lung cancer patients receiving immunotherapy.

Acute kidney disease in hospitalized pediatric patients: risk prediction based on an artificial intelligence approach.

Background: Acute kidney injury (AKI) and acute kidney disease (AKD) are prevalent among pediatric patients, both linked to increased mortality and extended hospital stays. Early detection of kidney injury is crucial for improving outcomes. This study presents a machine learning-based risk prediction model for AKI and AKD in pediatric patients, enabling personalized risk predictions.

Atractylodes macrocephala Koidz polysaccharide ameliorates DSS-induced colitis in mice by regulating the gut microbiota and tryptophan metabolism.

Background And Purpose: Ulcerative colitis (UC) is an idiopathic inflammatory bowel disease, and the range of current clinical treatments is not ideal. We previously found that polysaccharide of Atractylodes macrocephala Koidz (PAMK) is beneficial in DSS-induced colitis, and we aimed to investigate the underlying mechanisms in this study.

Experimental Approach: PAMK was used to treat DSS-induced colitis in mice, 16S rRNA sequencing analysis was used to detect changes in the intestinal microbiota, targeted metabolomics analysis was used to determine the content of tryptophan-metabolizing bacteria, and western blotting was used to determine aryl hydrocarbon receptor (AhR) and pregnane X receptor (PXR) levels.

The Golden Age of Thermally Activated Delayed Fluorescence Materials: Design and Exploitation.

Since the seminal report by Adachi and co-workers in 2012, there has been a veritable explosion of interest in the design of thermally activated delayed fluorescence (TADF) compounds, particularly as emitters for organic light-emitting diodes (OLEDs). With rapid advancements and innovation in materials design, the efficiencies of TADF OLEDs for each of the primary color points as well as for white devices now rival those of state-of-the-art phosphorescent emitters. Beyond electroluminescent devices, TADF compounds have also found increasing utility and applications in numerous related fields, from photocatalysis, to sensing, to imaging and beyond.

Feasibility and potential of intraoperative ultrasound in arthroscopy of femoroacetabular impingement.

This study aimed to evaluate the feasibility of using ultrasound for monitoring osteochondroplasty intraoperatively, determine the factors that interfere with ultrasound imaging and assess its influence on surgeon performance. Intraoperative ultrasonography was performed during osteochondroplasty in 39 cases of arthroscopy. The femoral head-neck junction (FHNJ) was evaluated using ultrasonography.

Constitutive Overexpression of CRZ1 in Reveals Its Ability to Enhance Recombinant Lipase Production.

The filamentous fungus is extensively utilized in the realm of recombinant protein expression owing to its well-established protein production systems. However, the potential for efficient and convenient protein production in has not been fully harnessed. To further increase the production of recombinant lipase lipase B (CalB), we overexpressed seven transcription activators and found that overexpression of the calcineurin CRZ1 could significantly enhance CalB production by 2.