Publications by authors named "Yan Xiao-Li"

To retard starch retrogradation and improve bread quality, a novel maltotetraose-forming amylase (AhMFA) from Atopomonas hussainii was expressed in Komagataella phaffii. After high cell density fermentation, the enzyme activity reached a maximum level of 3032 U mL. AhMFA showed optimal activity at pH 6.

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Background: To analyze the association between the hemoglobin glycation index (HGI) and the long-term prognosis of patients with coronary artery disease (CAD).

Methods: HGI represented the difference between laboratory measured Hemoglobin A1c (HbA1c) and predicted HbA1c based on a liner regression between Hb1Ac and fasting plasma glucose (FPG). A total of 10 598 patients who treated with percutaneous coronary intervention (PCI) were stratified into three groups (low HGI group: HGI<-0.

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For applications in food industries, a fungal α-amylase from Malbranchea cinnamomea was engineered by directed evolution. Through two rounds of screening, a mutant α-amylase (mMcAmyA) was obtained with higher optimal temperature (70 °C, 5 °C increase) and better hydrolysis properties (18.6 % maltotriose yield, 2.

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Background: Many imaging scoring models have been developed for tumor surgery to provide critical guidance for the selection of surgical methods. However, little research has been aimed at developing scoring models for adrenal tumors and retroperitoneal laparoscopic adrenal surgery (RLAS), which has become the primary technique for treating adrenal tumors. The study set out to establish a computed tomography (CT)-based adrenal tumor scoring model for predicting perioperative outcomes in patients with adrenal tumors who have undergone RLAS.

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Functional oligosaccharides exert obesity-reducing effects by acting at various pathological sites responsible for the development of obesity. In this study, tamarind xyloglucan oligosaccharides (TXOS) were used to attenuate metabolic disorders via the gut-liver axis in mice with high-fat-diet (HFD)-induced obesity, as determined through LC/MS-MS and 16S rRNA sequencing technology. A TXOS dose equivalent to 0.

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The present supply of prebiotics is entirely inadequate to meet their demand. To produce novel prebiotics, a d-mannose isomerase (MIaseA) from was first produced in (). MIaseA shared the highest amino acid sequence identity (58.

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Partially hydrolyzed konjac powder (PHKP) can be used to increase the daily intake of dietary fibers of consumers. To produce PHKP by enzymatic hydrolysis, a novel β-mannanase gene (McMan5B) from Malbranchea cinnamomea was expressed in Pichia pastoris. It showed a low identity of less than 52% with other GH family 5 β-mannanases.

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Pulmonary fibrosis (PF) is a progressive fibrosing disease, characterized by excessive accumulation of extracellular matrix (ECM) and remodeling of the lung architecture, which finally result in respiratory failure. Currently, there is no satisfactory treatment for PF, therefore, the development of effective agents is urgently needed. Lotus plumule, the green embryo of Gaertn.

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  • The study aimed to analyze the epidemiological features of major joint fracture-dislocations from 2015 to 2019 at a medical facility in Hebei, involving 582 patients with a higher prevalence in males (66.84%).
  • Results showed that hip and ankle fractures were the most common types, especially among men aged 25-34 years and women aged 45-54 years, respectively, with varying age groups displaying different predominant joint injuries.
  • The overall male-to-female ratio was 2.02:1, with shoulder injuries being more frequent in older adults (55-64 years) and notable differences in injury distribution by age and gender across all major joints.
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  • The xyloglucanase gene (RmXEG12A) from the fungus Rhizomucor miehei was successfully expressed in the yeast Pichia pastoris, showing a high enzyme activity of 25,700 U/mL through high cell density fermentation.
  • The enzyme performs best at pH 7.0 and a temperature of 65 °C, specifically breaking down xyloglucan with a notable activity rate.
  • Hydrolysis of tamarind powder produced xyloglucan oligosaccharides, which enhanced the water holding capacity of yogurt and significantly promoted the growth of beneficial bacteria in the yogurt.
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Objective: To investigate the epidemiological characteristics of major intra-articular fractures.

Methods: This retrospective study enrolled patients with major intra-articular fractures who were treated in the Third Hospital of Hebei Medical University from January 2015 to December 2019. A total of 11,084 patients (7,338 [66.

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To produce manno-oligosaccharides from cassia gum, a mutated glycoside hydrolase family 134 β-mannanase gene (mRmMan134A) from Rhizopus microsporus var. rhizopodiformis F518 was expressed in Pichia pastoris and a high expression level (3680 U mL) was obtained through high cell density fermentation. mRmMan134A exhibited maximum activity at pH 5.

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Dental implants represent the majority of treatment strategies used to replace missing teeth. However, peri-implant diseases caused by disturbance in peri-implant microbiological balance are among the reasons for implant failure. Since the 1980s, peri-implant microorganisms have been a hot research topic in dental microbiology.

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In the present study, the therapeutic effects and the underlying molecular mechanisms of microRNA (miR)‑145 were investigated in non‑small cell lung cancer (NSCLC) cells. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to examine miR‑145 expression. An MTT assay and flow cytometry were used to investigate cell proliferation and apoptosis, respectively.

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  • A field experiment was conducted using surface drip irrigation to assess how different levels of water and nitrogen influenced poplar tree growth and soil nitrogen content over two growing seasons.
  • Three irrigation levels and three nitrogen addition levels were tested, leading to significant increases in tree diameter, height, and stem volume, particularly when both water and nitrogen were maximally applied.
  • Increases in total nitrogen content in the soil were observed, showing a strong positive correlation with tree growth, although irrigation alone did not significantly impact tree size.
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  • Mannan, a component of hemicellulose from plants, is broken down by β-mannanases like the cloned enzyme RmMan134A from Rhizopus microsporus, which performs best at pH 5.0 and 50°C with high activity on locust bean gum.
  • The study analyzed the enzyme's substrate-binding mechanism by solving complex structures, revealing a total of at least seven subsites in its catalytic groove that interact with mannose and hinder bonding with galactose.
  • Various binding modes showed that certain subsites are essential for hydrolysis, while rational design efforts improved the enzyme's activity and conditions for optimal performance.
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  • - An engineered enzyme called β-mannanase (mRmMan5A) was successfully produced in the yeast Pichia pastoris, achieving a high expression level of 79,680 U/mL.
  • - The enzyme operates best at a pH of 4.5 and a temperature of 65°C, showing strong effectiveness in breaking down mannans.
  • - Using steam explosion to pretreat palm kernel cake (PKC), the mRmMan5A enzyme yielded 34.8 grams of manno-oligosaccharides per 100 grams of dry PKC, successfully demonstrating the potential for mass production with a total yield of 261.3 grams from 1.0 kg of dry PKC
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The present study determined the anticancer activity and its mechanism of microRNA‑133b on cell proliferation of cisplatin-induced non-small cell lung cancer cells. The expression of microRNA-133b cisplatin‑induced non-small cell lung cancer (NSCLC) tissue was lower than that of para-carcinoma tissue in patients. Overall survival of higher expression in cisplatin-induced NSCLC patients was higher than that of lower expression in cisplatin‑induced NSCLC patients.

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  • Researchers used Proton NMR and chemometrics to check if camellia oil (CAO) was mixed with cheaper vegetable oils.
  • They found that while PCA could group pure and impure CAO well, it struggled with identifying specific types of adulterated oils due to their similarities.
  • However, OPLS-DA provided over 90% accuracy in identifying the oil types, and PLS models effectively predicted the level of adulteration using only a few key markers from the data.
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  • A novel β-mannanase gene (RmMan5A) from the fungus Rhizomucor miehei was successfully expressed in yeast to produce partially hydrolyzed guar gum (PHGG), a dietary fiber.
  • After a 168-hour fermentation, the enzyme yielded a high activity level of 85,200 UmL, optimizing at pH 7.0 and 65°C.
  • The resulting PHGG had a significant dietary fiber content (90.6%) and was further fractionated into various forms with different molecular weights and sugar ratios, showcasing its structural diversity.
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Background: β-Mannanase randomly cleaves the β-1,4-linked mannan backbone of hemicellulose, which plays the most important role in the enzymatic degradation of mannan. Although the industrial applications of β-mannanase have tremendously expanded in recent years, the wild-type β-mannanases are still defective for some industries. The glycoside hydrolase (GH) family 5 β-mannanase (Man5A) from shows many outstanding properties, such as high specific activity and hydrolysis property.

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[One case of infection in esophagus in human].

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi

September 2016

This paper reports one case of infection in the esophagus in human.

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Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) are two common viral pathogens in acute lower respiratory tract infections (ALRTI). However, the association of viral load with clinical characteristics is not well-defined in ALRTI. To explore the correlation between viral load and clinical characteristics of RSV and HMPV in children hospitalized for ALRTI in Lanzhou, China.

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  • The study aimed to investigate the effects of the Yiqiyangyin formula on mice with advanced lung cancer cachexia.
  • A total of 60 mice were divided into five groups, including a normal control, model, and various treatment groups, with the effects measured through body weight, food intake, and serum cytokines after eight days of treatment.
  • Results showed that the Yiqiyangyin formula significantly improved certain serum markers and immune cell levels while reducing pro-inflammatory cytokines compared to the cisplatin group, indicating its potential to influence cancer cachexia through immune regulation.
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