Genome-Wide Identification and Analysis of Glycosyltransferases in .

Corn leaf blight and stem rot caused by are significant diseases that severely affect corn crops. Glycosyltransferases (GTs) catalyze the transfer of sugar residues to diverse receptor molecules, participating in numerous biological processes and facilitating functions ranging from structural support to signal transduction. This study identified 101 genes through functional annotation of the TZ-3 genome.

RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, .

Neuropeptide (abbreviated as ) is a recently discovered peptide that is present in many arthropods and is the ligand of the , a member of the G protein-coupled receptors (GPCRs) superfamily, which plays a regulatory role in diverse physiological processes such as feeding, circadian rhythm, insulin production, lipid metabolism, growth, and reproduction. However, the function of this gene in aphids is still unknown. Here, we characterized and determined the potential role of / signaling in the pea aphid, , which is a notorious pest in agriculture.

Apoptosis-Inducing and Proliferation-Inhibiting Effects of Doramectin on Mz-ChA-1 Human Cholangiocarcinoma Cells.

Cholangiocarcinoma is a malignant tumor that emerges in the intrahepatic or extrahepatic bile ducts. Doramectin (DOR), a third-generation derivative of avermectins (AVMs), is renowned for its low toxicity and high efficiency. However, no research has hitherto focused on the anti-cholangiocarcinoma effects of these drugs.

Comparative finite element analysis: internal fixation plate versus intramedullary nail for neer type III-VI proximal humeral fractur.

Background: Finite element analysis (FEA) could advance the understanding of fracture fixation and guide the choice of surgical treatment. This study aimed to compare two internal fixation methods in the treatment of displaced proximal humeral fracture (PHF) through FEA.

Methods: Three-dimensional FEA model based on the left shoulder joint of a 67-year-old female patient with PHFs and osteoporosis was adopted, in order to analyze the fixation effect and load stress distribution of internal fixation plates with open reduction and intramedullary nails without opening the fracture in the treatment of Neer III-VI PHF.

Mass Acquisition of Dirac Fermions in Bi_{4}I_{4} by Spontaneous Symmetry Breaking.

Massive Dirac fermions, which are essential for realizing novel topological phenomena, are expected to be generated from massless Dirac fermions by breaking the related symmetry, such as time-reversal symmetry in topological insulators or crystal symmetry in topological crystalline insulators. Here, we report scanning tunneling microscopy and angle-resolved photoemission spectroscopy studies of α-Bi_{4}I_{4}, which reveals the realization of massive Dirac fermions in the (100) surface states without breaking the time-reversal symmetry. Combined with first-principles calculations, our experimental results indicate that the spontaneous symmetry breaking engenders two nondegenerate edge states at the opposite sides of monolayer Bi_{4}I_{4} after the structural phase transition, imparting mass to the Dirac fermions after taking the interlayer coupling into account.

Sulfur dioxide increases testosterone biosynthesis by activating ERK1/2 pathway and disrupting autophagy in Leydig cells.

Sulfur dioxide (SO) is a ubiquitous environmental pollutant that has been shown to be toxic to the male reproductive system, but the underlying mechanism remains unclear. Therefore, the SO-treated mice and primary Leydig cell models were established to investigate the effects of SO on the production of testosterone and its specific mechanism. The results demonstrated that SO activated the ERK1/2 signaling pathway, leading to increased key proteins expression of testosterone biosynthesis and elevated testosterone levels.

Schistosoma sex-biased microRNAs regulate ovarian development and egg production by targeting Wnt signaling pathway.

Adult Schistosoma produces a large number of eggs that play essential roles in host pathology and disease dissemination. Consequently, understanding the mechanisms of sexual maturation and egg production may open a new avenue for controlling schistosomiasis. Here, we describe that Bantam miRNA and miR-1989 regulate Wnt signaling pathway by targeting Frizzled-5/7/9, which is involved in ovarian development and oviposition.

Precise identification of somatic and germline variants in the absence of matched normal samples.

Somatic variants play a crucial role in the occurrence and progression of cancer. However, in the absence of matched normal controls, distinguishing between germline and somatic variants becomes challenging in tumor samples. The existing tumor-only genomic analysis methods either suffer from limited performance or insufficient interpretability due to an excess of features.

Factors influencing vancomycin trough concentration in burn patients: a single center retrospective study.

Objective: To analyze factors influencing the vancomycin trough concentration in burn patients to provide a basis for the more rational use of vancomycin in these patients.

Materials And Methods: We collected the clinical data of adult burn patients treated with vancomycin in a Chinese hospital. Vancomycin was administered at a dosing regimen of 1.

The effect of toluene sulfonic acid remimazolam on postoperative delirium in elderly patients undergoing painless bronchoscopy.

Toluene sulfonic acid remimazolam is a novel benzodiazepine that differs from traditional benzodiazepines (BZDs) due to its rapid onset, swift metabolism, and lack of hepatic or renal metabolism, as well as its reduced effects on cardiac and cerebral functions. Despite its potential advantages, clinical experience with this agent remains limited. This study investigated the effect of remizolam on postoperative delirium in elderly patients undergoing painless bronchoscopy.

FXYD1 was identified as a hub gene in recurrent miscarriage and involved in decidualization via regulating Na/K-ATPase activity.

Purpose: Recurrent miscarriage (RM) is a distressing and complicated adverse pregnancy outcome. It is commonly recognized that insufficient decidualization could result in RM, but the molecular mechanisms of decidual impairment are still not fully understood. Thus, this study aimed to identify novel key genes potentially involved in RM and explore their roles played in endometrial decidualization.

PIEZO1 Affects Cell Growth and Migration via Microfilament-Mediated YAP trans-Latitudinal Regulation.

Environmental mechanical forces, such as cell membrane stress, cell extrusion, and stretch, have been proven to affect cell growth and migration. Piezo1, a mechanosensitive channel protein, responds directly to endogenous or exogenous mechanical stimuli. Here, we explored the Piezo1 distribution and microfilament morphological changes induced by mechanical forces in the tumor and normal cells.

Strong Heteroatomic Bond-Induced Confined Restructuring on Ir-Mn Intermetallics Enable Robust PEM Water Electrolyzers.

Low-iridium acid-stabilized electrocatalysts for efficient oxygen evolution reaction (OER) are crucial for the market deployment of proton exchange membrane (PEM) water electrolysis. Manipulating the in situ reconstruction of Ir-based catalysts with favorable kinetics is highly desirable but remains elusive. Herein, we propose an atomic ordering strategy to modulate the dynamic surface restructuring of catalysts to break the activity/stability trade-off.

Histochemical assessment of the femora of Spontaneously Diabetic Torii-Lepr (SDT-fa/fa) rats that mimic type II diabetes.

Objective: To elucidate the mechanisms underlying diabetic osteoporosis, we conducted a comprehensive histological examination of the femora of Spontaneously Diabetic Torii-Lepr (SDT-fa/fa) rats, an established model of obesity-related type 2 diabetes.

Materials And Methods: Femora from 12 30-week-old male SDT-fa/fa rats and age-matched Sprague-Dawley (SD) rats (controls) were used for detailed histochemical analyses, including tartrate-resistant acid phosphatase (TRAP), cathepsin K, alkaline phosphatase (ALP), phosphoethanolamine/ phosphocholine phosphatase 1 (PHOSPHO1), dentin matrix protein (DMP)-1, matrix extracellular phosphoglycoprotein (MEPE), sclerostin, osteocalcin staining, silver impregnation, von Kossa staining, and micro-computed tomography (CT).

Results: Micro-CT and hematoxylin-eosin staining demonstrated significantly reduced trabecular bone volume in the femoral metaphyses of SDT-fa/fa rats.

Individual and joint associations between sleep duration and physical activity with cognitive function: A longitudinal analysis among middle-aged and older adults in China.

Introduction: Studies using cross-sectional data or with a short follow-up period fail to distinguish whether the associations between sleep duration and physical activity with cognitive function result from reverse causation.

Methods: The longitudinal study examined the individual and joint associations, with specific temporality, between sleep duration and physical activity with cognitive function, using time-lagged linear mixed models and generalized additive mixed models.

Results: A total of 14,694 participants aged ≥ 50 years were included, with an average lagged time of 4.

Epidermal stem cell-derived exosomes improve wound healing by promoting the proliferation and migration of human skin fibroblasts.

Background: Epidermal stem cells (ESCs) are primarily located in the basal layer of the epidermis and play a crucial role in wound healing. ESCs-derived exosomes (ESCs-Exo) are emerging as promising candidates for skin regeneration and wound healing. However, the underlying mechanisms remain unclear.

The case report of the impact of coach self-supportive behaviors on the communication effectiveness with depressed athletes: The mediating role of intrinsic motivation.

Rationale: Coach autonomy-supportive behaviors are a crucial factor in the communication effectiveness with athletes experiencing depressive moods. Therefore, clarifying the relationship between coach autonomy-supportive behaviors and the communication effectiveness with athletes experiencing depressive moods is of significant importance for promoting effective communication between coaches and athletes. This study employs in-depth interviews, questionnaires, and stepwise regression analysis, using purposive sampling to investigate coaches and athletes experiencing depressive moods from various regions, levels, genders, and sports in China.

TEAD3 and TEAD4 play overlapping role in bovine preimplantation development.

During mammalian preimplantation development, the transition from morula to blastocyst is a critical biological event. This process involves polarization and initial specification of lineages, regulated by various transcription factors that have been extensively studied in mice. Our single-cell RNA sequencing analyses revealed that TEAD3 is specifically expressed in the trophectoderm cells of bovine preimplantation embryos, unlike in mice.

Study on the fusion of improved YOLOv8 and depth camera for bunch tomato stem picking point recognition and localization.

When harvesting bunch tomatoes, accurately identifying certain fruiting stems proves challenging due to their obstruction by branches and leaves, or their similarity in colour to the branches, main vines, and lateral vines. Additionally, irregularities in the growth pattern of the fruiting pedicels further complicate precise picking point localization, thus impacting harvesting efficiency. Moreover, the fruit stalks being too short or slender poses an obstacle, rendering it impossible for the depth camera to accurately obtain depth information during depth value acquisition.

Development and Validation of the Predictive and Prognostic ChemoResist Signature in Resected Pancreatic Ductal Adenocarcinoma: Multicenter Study.

Objective: To develop and validate a signature to precisely predict prognosis in pancreatic ductal adenocarcinoma (PDAC) undergoing resection and adjuvant chemotherapy.

Summary Background Data: PDAC is largely heterogeneous, and responds discrepantly to treatment.

Methods: 551 consecutive patients with PDAC from 3 tertiary centers were initially enrolled.

Genetic profile in primary tumor tissue of advanced lung adenocarcinoma patients with adrenal metastasis.

The aim of this study was to examine the genomic characteristics and explore the molecular mechanisms underlying adrenal metastases in lung adenocarcinoma. 57 patients diagnosed with lung adenocarcinoma (LUAD) and adrenal metastases (AM) were enrolled, alongside 33 controls diagnosed with non-adrenal metastases (non-AM) at the time of diagnosis. The primary lung cancer tissue sample were analyzed using next-generation sequencing.

Radiolabelled anti-PD-L1 peptide PET/CT in predicting the efficacy of neoadjuvant immunotherapy combined with chemotherapy in resectable non-small cell lung cancer.

Background: This study aimed to evaluate the predictive value of baseline PD-L1 targeted peptide Ga-NOTA-WL12 PET/CT in neoadjuvant immunotherapy combined with chemotherapy of resectable NSCLC.

Methods: Patients with resectable NSCLC (n = 20) enrolled in this prospective study received baseline paired Ga-NOTA-WL12 PET/CT and F-FDG PET/CT. After 2-4 cycles of toripalimab plus nab-paclitaxel and cisplatin, surgery was performed if R0 resection was available.