Analysis of microbial methane oxidation capacity of landfill soil cover using quorum sensing.

Landfill gas (LFG) has become the second-largest anthropogenic source of methane (CH) emissions globally. CH is the second most significant greenhouse gas after carbon dioxide (CO), thus it is crucial to mitigate the methane emission of landfills. The soil in landfill cover layers is rich in methane-oxidizing bacteria (MOB), which use CH as their sole carbon and energy source.

Compression and water retention behavior of saline soil improved by MICP combined with activated carbon.

Saline soil is widely distributed in China and poses significant challenges to engineering construction due to its harmful effects, such as salt heaving, dissolution collapse, and frost heaving. The Microbial-Induced Calcite Precipitation (MICP) method is an emerging environmental-friendly modification that can reduce or eliminate the environmental and engineering hazards of saline soil. To verify the feasibility of the MICP method for improving the properties of saline soil, laboratory tests were conducted to study the effects of salt content, activated carbon content and freeze-thaw cycles on the compression and water retention behavior of MICP modified saline soil.

3D Zn-Based coordination polymer: Synthesis, structure and fluorescent sensing property for nitroaromatic compounds.

A water-stable Zn-based coordination polymer (CP) with excellent photophysical behavior, namely [ZnL(atez)(HO)] (compound 1; HL = 4-(2',3'-dicarboxylphenoxy); atez = 5-aminotetrazole), was successfully prepared by the solvothermal reaction of Zn ions with a π-conjugated and semi-rigid multicarboxylate ligand HL in the presence of N-containing linker atez. Compound 1 displays a hierarchically pillared three-dimensional (3D) (3,4,5)-connected (4·6) (4·6) (4·6·8) net which is based on two-dimensional (2D) multicarboxylate- Zn layers strutted by the atez ligands. Sensing investigations of compound 1 reveal that this material can selectively and sensitively detect nitroaromatic compounds in water suspension through fluorescence quenching effect.

Multifunctional Cd-CP for fluorescence sensing of Cr(VI), MnO, acetylacetone and ascorbic acid in aqueous solutions.

The development of multifunctional fluorescent chemosensors for the detection of multiple targets remains challenging but of great importance. In this paper, one novel coordination polymer (CP), denoted as [Cd(edda)(phen)]∙HO (compound 1, Hedda = 5,5' (ethane-1,2-diylbis(oxy)) diisophthalic acid, phen = 1,10-phenanthroline) is successfully designed and prepared under hydrothermal conditions. Structural analysis indicates that compound 1 possesses a one-dimensional (1D) double chain structure, then self-assembles into a three-dimensional (3D) supramolecular framework via π…π interactions between phen molecules.

A Brief Report on the Collapse of Self-Built Houses on 29 April 2022, in Changsha, China.

A self-built house collapsed in Changsha City, Hunan Province, on 29 April 2022, causing 53 people to be trapped and 39 people to go missing. The tragedy caused a huge loss of life, so the stability of self-built houses has a non-negligible impaction on public safety. This report presents causes of the incident, rescue operations, cause analysis, and the analytic hierarchy process (AHP).

Cause Analysis and Preventive Measures of Guizhou D2809 Train Derailment Accident in Guizhou, China on 4 June 2022.

This paper summarizes the cause of the debris flow impact train accident by investigating the local geological condition, meteorological data and field investigation that happened in Guizhou, China on 4 June 2022. The result showed that the major reason is the continuous heavy rain in the surrounding area, which led to a small landslide at the upper right of the tunnel entrance. Besides, the construction of the Jianrong Expressway in the upper reaches increased the catchment area, which makes the water content of the upper soil increase while the shear strength decreases.

A water-stable dual-responsive Cd-CP for fluorometric recognition of hypochlorite and acetylacetone in aqueous media.

One novel cadmium(II)-coordination polymer [CdL(datrz)(HO)] (CP 1) is controllably synthesized by surmising the astute combination of semi-rigid tricarboxylate acid 4-(2',3'-dicarboxylphenoxy) benzoic acid (HL) and auxiliary ligand 3,5-diamino-1,2,4-triazole (datrz). Structure analysis shows that CP 1 has a two-dimensional (2D) layer structure with a 5-nodal (4) (4·6) (4·6·8) (4·6) (4·6·8) topology. Further investigations reveal that CP 1 shows superordinary water stability and good thermal stability.

A dual luminescent sensor coordination polymer for simultaneous determination of ascorbic acid and tryptophan.

Simultaneous high sensitivity detection of biomolecules is important for research in medicine, living cells and environmental samples. In this work, a water stable coordination polymer, [Cd(bptc)(4,4'-bpy)(HO)]ˑHO 1 (Hbptc = 2,3,3',4'-biphenyl tetracarboxylic acid, 4,4'-bpy = 4,4'-bipyridine), was designed and successfully synthesized as a luminescent sensor for simultaneous recognition of Ascorbic Acid (AA) and L-Tryptophan (L-Trp) based on luminescent -OFF and -ON, respectively. Importantly, the proposed sensing system showed an excellent performance with high K values of 4.

Ln-CPs constructed from unsymmetrical tetracarboxylic acid ligand: Tunable white-light emission and highly sensitive detection of CrO, CrO, MnO in water.

A series of isostructural lanthanide coordination polymers (Ln-CPs), [Ln(Hbptc)(HO)]·HO [Ln = Er (1), Pr (2), Dy (3), Sm (4), Gd (5), Nd (6) and Tb(7); Hbptc = 2,3,3',4'-biphenyl tetracarboxylic acid] have been isolated based on an unsymmetrical tetracarboxylic acid. Single-crystal X-ray diffraction analysis reveals that all CPs featured a two dimensional (2D) layer with (6, 6, 6)-connected 6 topology. Luminescent spectra demonstrate that CPs 1-7 exhibit impressive UV-visible luminescence in the solid state at room temperature.

[Prezygotic reproductive isolation of two sympatric species of Ligularia weeds in eastern Qinghai-Tibet Plateau, China].

Reproductive isolation, the key process to prevent interspecific hybridization and keep the completeness and independence of species, is crucial to the formation and maintenance of biodiversity. The forming steps, methods and intensities of reproductive isolation between different species are not completely the same. Literatures on reproductive isolation between species provide valuable evidence for speciation and its maintenance.

New in situ generated acylhydrazidate-coordinated complexes and acylhydrazide molecules: Synthesis, structural characterization and photoluminescence property.

By utilizing the hydrothermal in situ acylation of organic acids with N2H4, three acylhydrazidate-coordinated compounds [Mn(L1)2(H2O)2] (L1=2,3-quinolinedicarboxylhydrazidate; HL1=2,3-dihydropyridazino[4,5-b] quinoline-1,4-dione) 1, [Mn2(ox)(L2)2(H2O)6]·2H2O (L2=benzimidazolate-5,6-dicarboxylhydrazide; HL2=6,7-dihydro-1H-imidazo[4,5-g]phthalazine-5,8-dione; ox=oxalate) 2, and [Cd(HL3)(bpy)] (L3=4,5-di(3'-carboxylphenyl)phthalhydrazidate; H3L3=6,7-dihydro-1H-imidazo[4,5-g]phthalazine-5,8-dione; bpy=2,2'-bipyridine) 3, as well as two acylhydrazide molecules L4 (L4=oxepino[2,3,4-de:7,6,5-d'e']diphthalazine-4,10(5H,9H)-dione) 4 and L5 (L5=4,5-dibromophthalhydrazide; L5=6,7-dibromo-2,3-dihydrophthalazine-1,4-dione) 5 were obtained. X-ray single-crystal diffraction analysis reveals that (i) 1 only possesses a mononuclear structure, but it self-assembles into a 2-D supramolecular network via the NhydrazineH⋯Nhydrazine and OwH⋯Ohydroxylimino interactions; (ii) 2 exhibits a dinuclear structure. Ox acts as the linker, while L2 just serves as a terminal ligand; (iii) In 3, L3 acts as a 3-connected node to propagate the 7-coordinated Cd(2+) centers into a 1-D double-chain structure; (iv) 4 is a special acylhydrazide molecule.

Angiotensin II Destabilizes Coronary Plaques in Watanabe Heritable Hyperlipidemic Rabbits.

Objective: Increased plasma concentrations of angiotensin II (Ang II) have been implicated in many cardiovascular diseases, such as atherosclerosis, aortic aneurysms, and myocardial infarction, in humans. However, it is not known whether high levels of plasma Ang II affect coronary plaque stability and subsequent myocardial infarction. This study was designed to examine whether elevated plasma Ang II can directly induce coronary events, such as acute coronary syndrome.

A new three-dimensional Zn(2+) coordination polymer constructed from oxalate and 1,2,4-triazolate.

A new 3-D Zn(2+) coordination polymer (CP) [(CH3)2NH2]3[Zn6(ox)4.5(trz)6]⋅4H2O (ox=oxalate; trz=1,2,4-triazolate) 1 was obtained by a simple solvothermal self-assembly. The crystal structural analysis demonstrates that the trz molecules link the Zn(2+) ions into a two-dimensional (2-D) layer network, which is based on the trinuclear Zn3(trz)6 clusters.

5-(3',4'-Dicarboxylphenoxy)isophthalate/5-(2',3'-Dicarboxylphenoxy)isophthalate-Based 3D Cadmium(II) Coordination Polymers: Synthesis, Structure, and Sensing of Nitrobenzene.

5-(3',4'-Dicarboxylphenoxy)isophthalic acid (H L1) and 5-(2',3'-dicarboxylphenoxy)isophthalic acid (H L2) were reacted with Cd salt with/without the assistance of N-donor ligands, creating five 3D Cd coordination polymers [Cd (L1)(H O) ]⋅H O (1), [Cd (L1)(bpe)(H O)] (bpe=1,2-bis(4-pyridyl)ethene; 2), [Cd (L1)(bpa)(H O)] (bpa=1,2-bis(4-pyridyl)ethane; 3), [Cd (L1)(bpp)] (bpp=1,3-bis(4-pyridyl)propane; 4), and [Cd (L2)(bpp) (H O) ]⋅H O (5). Crystal-structure analysis reveals 1) in 1, phthalate moieties for L1 link Cd1 centers into a 1D slightly helical chain; Cd2 centers act as bridges, linking 1D helical chains into a 3D network with a (3,5)-connected topology; 2) in 2-4, although Cd centers were further modified by the introduction of organic bases, L1 molecules still link Cd centers into 3D networks, which can all be simplified as pcu topology; 3) in 2-4, phthalate moieties first link Cd centers into an oligomer, in which carboxylate-bridged discrete or rod-shaped secondary building units (SBUs) are found (tetranuclear SBU in 2 and 3, rod-shaped SBU in 4); and 4) in 5, L2 molecules link Cd centers into a 2D (4,4) net; introduced bpp molecules serve as pillar linkers, extending Cd -L2 layers into a 3D network of 5. The sensing abilities of 2-5 for nitrobenzene (NB) were investigated.

New 1-D and 3-D thiocyanatocadmates modified by various amine molecules and Cl(-)/CH3COO(-) ions: synthesis, structural characterization, thermal behavior and photoluminescence properties.

Under ambient conditions, reactions of CdCl2/Cd(CH3COO)2, SCN(-) and various organic amine molecules in strongly acidic solutions afforded the five new thiocyanatocadmates [H2(abpy)][CdCl2(SCN)2] (abpy = azobispyridine) 1, [H(apy)][Cd(SCN)3] (apy = 4-aminopyridine) 2, [H(ba)]2[CdCl2(SCN)2] (ba = tert-butylamine) 3, [H2(tmen)][Cd3Cl6(SCN)2] (tmen = N,N,N',N'-tetramethylethylenediamine) 4, and [H(dba)]2[Cd2(CH3COO)2(SCN)4] (dba = dibutylamine) 5. In compound 2 only, the CH3COO(-) ions in Cd(CH3COO)2 were completely displaced by SCN(-), producing a chained thiocyanatocadmate [Cd(SCN)3](-). In the other four compounds, the Cl(-) or CH3COO(-) ions appeared in the final inorganic anion frameworks.

4-(4-carboxyphenoxy)phthalate-based coordination polymers and their application in sensing nitrobenzene.

Under hydro(solvo)thermal conditions, two 4-(4-carboxyphenoxy)phthalate-based three-dimensional (3-D) coordination polymers: [Cd3(cpph)2(bpa)2(H2O)]·0.5H2O (cpph = 4-(4-carboxyphenoxy)phthalate, bpa = 1,2-bis(4-pyridyl)ethane) 1, and [H2(bpp)][Mn2(cpph)2(H2O)2] (bpp = 1,2-bis(4-piperidyl)propane) 2 were isolated. In both compounds, the cpph molecules extend the metal ions into a 3-D network: a (4,6)-connected net for 1 and a simple 6-connected net with a pcu topology for 2.

New Zn²⁺ coordination polymers constructed from acylhydrazidate molecules: synthesis and structural characterization.

By employing two types of hydrothermal in situ ligand reactions (acylation of N2H4 with aromatic polycarboxylic acids, reduction of 3-nitrophthalhydrazide by N2H4), three new acylhydrazidate-extended Zn(2+) coordination polymers [Zn2(3-apth)(atrz)2] (3-apth = 3-aminophthalhydrazidate; atrz = 3-amino-1,2,4-triazolate) 1, [Zn2(4-apth)(atez)2] (4-apth = 4-aminophthalhydrazidate; atez = 5-aminotetrazolate) 2, and [Zn(3-cppth)(H2O)] (3-cppth = 4-(3-carboxyphenoxy)phthalhydrazidate) 3 were obtained. X-ray single-crystal diffraction analysis revealed that (i) compound 1 possesses a 3-D structure. The triazolate molecules link the Zn(2+) ions to form a 2-D layer with a (6,3) topology.

Construction of acylhydrazidate-extended metal-organic frameworks.

Under hydrothermal conditions, the reactions of Ba(2+)/Zn(2+), aromatic polycarboxylic acids and N2H4 with or without oxalic acid were carried out, affording four new acylhydrazidate-extended metal-organic frameworks (MOFs) [Ba(pmdh)] (pmdh = pyromellitdihydrazidate) 1, [Ba(sdpth)(H2O)2]·0.5H2O (sdpth = 4,4'-sulfoyldiphthalhydrazidate) 2, [Ba2(cpth)2(H2O)2] (cpth = 4-carboxylphthalhydrazidate) 3 and [Zn2(pdh)2(ox)]·H2O (ox = oxalate, pdh = pyridine-2,3-dicarboxylhydrazidate) 4. The acylhydrazidate molecules pmdh, sdpth, cpth and pdh in compounds 1-4 derived from the hydrothermal in situ acylation of N2H4 with aromatic polycarboxylic acids.

Synthesis, structural characterization and photoluminescence property of three Zn(2+)/Mn(2+)-acylhydrazidate complexes and two acylhydrazide molecules.

By employing the hydrothermal in situ acylation of N2H4 with aromatic polycarboxylic acids, three new acylhydrazidate-containing complexes [Zn(N2H4)(dphkh)]·H2O (dphkh = 4,4'-diphthalhydrazidatoketone hydrazone) 1, [Zn(npth)2] (npth = naphthalhydrazidate) 2 and [Mn(mpdh)2(H2O)2]·2H2O 4, and two new acylhydrazide molecules [bpth]·0.5H2O (bpth = 3,3'-biphthalhydrazide) 3 and [(chpth)2] (chpth = 4-chloro-5-hydrazinophthalhydrazide) 5 were obtained. It is noteworthy that (i) compound 1 is a layered Zn(2+) coordination polymer with a mixed ligand of dphkh and N2H4.

New Cd2+, Pb2+ complexes with acylhydrazidate molecules from in situ acylation reactions.

Through employing the hydrothermal in situ acylation of N2H4 with aromatic dicarboxylic acids, four new acylhydrazidate-coordinated complexes, [Cd2(pth)4(phen)2(H2O)2]·H2O (pth = phthalhydrazidate; phen = 1,10-phenanthroline) 1, [Cd3(dcpth)6(phen)2(H2O)2] (dcpth = 4,5-dichlorophthalhydrazidate) 2, [Cd(pdh)2(H2O)2] (pdh = pyridine-2,3-dicarboxylhydrazidate) 3 and [Pb(sdpth)(phen)] (sdpth = 4,4'-sulfoyldiphthalhydrazidate) 4, were obtained. X-ray analysis revealed that (i) isomerization and deprotonation of acylhydrazide molecules also occurred; (ii) compound 2 possesses a one-dimensional (1-D) double-chain structure, in which dcpth exhibits three types of coordination modes; (iii) compound 4 shows a 1-D castellated single-chain structure; (iv) the adjacent uncoordinated acylamino groups form a hydrogen-bonded dimer, via which compounds 2-4 self-assemble into different two-dimensional (2-D) supramolecular networks. In the solid state, only compound 3 emits green light (λ(em) = 525 nm), while in an aqueous solution, compounds 1, 3 and 4 all emit light.

New thiocyanatocadmates templated by multi-dentate N-heterocyclic/diamine molecules.

With diverse multi-dentate N-heterocyclic/diamine molecules as the cations, the reactions between Cd(2+) and SCN(-) in C2H5OH/H2O solution acidified by H2SO4 created five new thiocyanatocadmates [H2(bim)][Cd(SCN)2(H2O)2]SO4 (bim = 2,2'-biimidazole) 1, [H2(pympip)]2[Cd2(SCN)4(SO4)2(H2O)4]·2H2O (pympip = 1-(2-pyrimidyl)piperazine) 2, [H2(tdpy)]2[Cd3(SCN)10] (tdpy = 4,4'-thiodipyridine) 3, [H2(badpm)][Cd(SCN)4] (badpm = 4,4'-biaminodiphenylmethane) 4 and [H2(pdma)][Cd2(SCN)4(SO4)] (pdma = 1,4-phenylenedimethanamine) 5. It is noteworthy that (i) in compounds 1, 2 and 5, SO4(2-) was incorporated into the organically templated thiocyanatocadmate frameworks; (ii) compound is a rare example, in which two types of chained thiocyanatocadmates are found, and (iii) compound shows an interesting layer structure, constructed up from the ribboned thiocyanatocadmates by μ2-mode SO4(2-). Photoluminescence analysis revealed that compounds 1, 2 and 5 in solid state emit blue light with the maximum at 425 nm for 1, 405 nm for 2 and 445 nm for 5, respectively.

HCV-NS3 Th1 minigene vaccine based on invariant chain CLIP genetic substitution enhances CD4(+) Th1 cell responses in vivo.

Background: HCV is a major cause of chronic liver disease, cirrhosis, hepatocellular carcinoma and death of end-stage liver disease worldwide. Therapy regimen based on IFN-alpha combined with ribavirin can induce a sustained virological and biochemical response in 20-60% of treated patients depending on the HCV genotype, the virus load and the age of the patients. So the development of a preventive or a therapeutic vaccine is very desirable.

Target HCV NS3 CD4+ Th1 epitope to major histocompatibility complex class II pathway.

A hepatitis C virus (HCV) plasmid vaccine was constructed, based on class II-associated invariant chain peptide (CLIP) substitution which endogenously targets HCV non-structure protein 3 (NS3) CD4+ T helper 1(Th1) epitope (1248AA-1261AA) to major histocompatibility complex (MHC) class II antigen. The in vitro expression results demonstrated that the vaccine was expressed efficiently in COS-7 cell line. The expressed protein could co-localize in endo-membrane system with BALB/c mouse MHC class II molecule I-Ad.

[A study on the inhibitory effect on transplanted tumor growth expressing HCV NS3 protein by HCV minigene vaccine based on invariant chain substitution in mice].

Objectives: To establish a transplanted tumor producing HCV NS3 protein in mice and study the therapeutic effect of minigene vaccine based on invariant chain substitution.

Methods: SP2/0-NS3 cells expressing HCV NS3 antigen were injected subcutaneously into BALB/ c mice. After three days of inoculation, different therapeutical reagents were injected intramuscularly into different groups of mice.