In-Tip Nanoreactors for Simultaneous Proteolysis and Enrichment of Phosphorylated Peptides.

Protein phosphorylation introduces negative charges on the hydroxyl groups of serine, threonine, and tyrosine residues, reducing the ionization efficiency of phosphorylated peptides. The low abundance of phosphorylated peptides often diminishes their detection using mass spectrometry. To enhance the identification of the low-abundance peptides, an enrichment step was often used, which complicated the high-throughput analysis of phosphorylated proteomes.

Tea Extracellular Vesicle-Derived MicroRNAs Contribute to Alleviate Intestinal Inflammation by Reprogramming Macrophages.

The clinical use of conventional medications for inflammatory bowel disease (IBD) is often limited by significant side effects. The extracellular vesicles derived from plant-based diets have shown promise in mitigating disease. Here, we discovered that natural extracellular vesicles from tea (TEVs) can achieve an appropriate transition from proinflammatory (M1) to anti-inflammatory (M2) macrophages and inhibit inflammation response both in vitro and in vivo.

3D microporous metal-organic framework Ca-TCPB as a fiber coating for headspace solid-phase microextraction of polycyclic aromatic hydrocarbons.

Polycyclic aromatic hydrocarbons (PAHs) as a type of persistent organic pollutants in environment can pose serious threat to ecosystem and human health. Herein, a microporous metal-organic framework (MOF) material namely Ca-TCPB [HTCPB: 1,2,4,5-tetrakis(4-carboxyphenyl)-benzene] with three-dimensional (3D) structure was synthesized via simple solvothermal method. The Ca-TCPB served as fiber coating of solid-phase microextraction (SPME) to extract and enrich PAHs prior to gas chromatography analysis.

Deep Eutectic Solvent-Based Ultrasound-Assisted Extraction of Flavonoids from .

Recently, deep eutectic solvents (DESs) have attracted much attention in the extraction and separation field because of their green characteristics, and they are widely used to extract various bioactive substances from plants. In this study, ten choline chloride (ChCl)-based mixtures were selected for extracting flavonoids from Houttuynia cordata. Then, the optimal conditions for the DES-based extraction of flavonoids (HCFs) were determined through one-way experiments and response surface optimization (RSM).

Prognostic significance of left atrial volume index in patients with severe aortic stenosis after transcatheter aortic valve replacement.

Background: Transcatheter aortic valve replacement (TAVR) is a critical approach for treating patients with severe aortic stenosis (AS). A staging cardiac damage has been recently proposed that provided prognostic value in patients underwent TAVR, and left atrial volume index (LAVi) is a significant indicator of left heart damage. This study aimed to investigate the prognostic significance of LAVi in patients with severe AS after TAVR.

RNA-Seq and ChIP-Seq Identification of Unique and Overlapping Target Genes and Pathways Regulated by TBX4 in Human Pulmonary Fibroblasts and Pericytes.

Transcription factor rare variants associate with pulmonary arterial hypertension (PAH), particularly in children, and are the second most common cause of heritable PAH. However, TBX4's down-stream targets and the molecular and cellular pathways these targets regulate remain largely unknown in PAH. We combined RNA-seq and ChIP-seq results to identify TBX4 direct targets in lung fibroblasts and pericytes, respectively.

Implementation of an Evidence-Based Nursing Protocol for Mechanical Complications of Enteral Nutrition: A Randomized Controlled Trial.

Background: Patients admitted to the intensive care unit (ICU) are at risk for malnutrition. To provide sufficient nutritional support, enteral nutrition (EN) is the preferred method for patients admitted to the neurology ICU.

Purpose: The purpose of this study was to examine the clinical effectiveness of an evidence-based nursing protocol for preventing and managing mechanical complications of EN for patients admitted to the neurology ICU.

Long-term Pu-erh tea alleviates inflammatory bowel disease via the regulation of intestinal microbiota and maintaining the intestinal mucosal barrier.

Unlabelled: Inflammatory bowel disease (IBD) is a chronic gastrointestinal condition with increasing global prevalence. Current therapies are limited, leading to exploration of novel treatments like Pu-erh tea, a fermented tea recognized for its health benefits. This study shows that long-term consumption of Pu-erh tea significantly reduces IBD symptoms in DSS-induced mice by moderating inflammation and enhancing oxidative responses in the colon.

Covalent Organic Framework Nanofilm-Assisted Laser Desorption Ionization Mass Spectrometry for the Determination of Benzophenone Derivatives in Personal Care Products.

Rational: Benzophenone derivatives, commonly used as UV filters in personal care products (PCPs), are widely prevalent and raise concerns due to their endocrine-disrupting effects. Sensitive and efficient analytical methods are in demand for their detection. In this study, we developed a TAPB-DMTP-covalent organic framework (COF) nanofilm-assisted laser desorption ionization mass spectrometry (LDI-MS) method for the quantitative analysis of 2,4-dihydroxybenzophenone (BP-1) in PCPs.

Chinese herbal formula Tongxie Yaofang granules for diarrhoea-predominant irritable bowel syndrome: a randomised, double-blind, placebo-controlled, phase II trial.

Objectives: To assess the therapeutic effects and safety of Tongxie Yaofang (TXYF) granules vs placebo as an alternative treatment for diarrhoea-predominant irritable bowel syndrome (IBS-D). We hypothesised that TXYF would improve clinical responses among patients with IBS-D.

Design: A randomised, double-blind, placebo-controlled, phase II, superiority trial.

A Comparison of the Predictive Value of International Medullary Thyroid Carcinoma Grading System (IMTCGS) With That of Other Risk Factors in a Chinese Medullary Thyroid Carcinoma Cohort.

Background: The International Medullary Thyroid Carcinoma Grading System (IMTCGS) was recently introduced in medullary thyroid carcinoma (MTC). This study aimed to assess the predictive value of the IMTCGS for disease response and survival, and compare its predictive ability with that of other traditional risk factors in a Chinese MTC cohort.

Methods: The data of 137 MTC patients undergoing initial surgery between January 2004 and June 2023 were included for analysis.

Effect of Ankle-Foot Orthosis on Paretic Gastrocnemius and Tibialis Anterior Muscle Contraction of Stroke Survivors During Walking: A Pilot Study.

Ankle-foot orthoses (AFOs) have been commonly prescribed for stroke survivors with foot drop, but their impact on the contractions of paretic tibialis anterior (TA) and medial gastrocnemius (MG) has remained inconclusive. This study thus investigated the effect of AFOs on these muscle contractions in stroke survivors. The contractions of paretic TA and MG muscles were assessed in twenty stroke patients and compared between walking with and without AFOs, using a novel wearable dynamic ultrasound imaging and sensing system.

Association between thyroid hormone sensitivity and carotid plaque risk: a health examination cohort-based study.

Introduction: The involvement of thyroid hormone in cardiovascular disease remains debated. The aim of our research was to ascertain whether thyroid hormone sensitivity indices are related to carotid plaque (CAP) risk in the general population.

Methods: We recruited 5,360 participants for health examinations to explore the correlation between thyroid hormone sensitivity indices and CAP risk.

Clinical Characteristics, Risk Factors, and Predictors of Nonobese Fatty Liver Disease: A Cross-Sectional Study.

Purpose: This study aimed to investigate the risk factors and predictors of non-obese fatty liver disease in the Chinese population.

Patients And Methods: A total of 6,014 adults who underwent physical examinations at Union Hospital of Huazhong University of Science and Technology from March 2019 to March 2023 were included in this study. Fatty liver disease was diagnosed based on at least two of the following criteria: diffuse echo patterns relative to the liver, spleen, and kidney; ultrasonic beam attenuation; and poor intrahepatic visual details.

Rapidly in situ forming antibiotic-free injectable hydrogel wound dressing for eradicating drug-resistant bacterial infections in human skin organoids.

The rising prevalence of global antibiotic resistance evokes the urgent requirement to explore the alternative antimicrobial candidates. It is of great significance to overcome these serious threats of multidrug-resistant bacterial infections and difficult-to-heal cutaneous wounds to human health. Herein, we proposed a rapidly in situ forming innovative antibiotic-free hydrogel dressing with excellent biocompatibility, easy injectability, strong tissue adhesion and superior antibacterial activity against drug-resistant bacteria.

Role of mitophagy in intervertebral disc degeneration: A narrative review.

Objective: The pivotal role of mitophagy in the initiation and progression of intervertebral disc (IVD) degeneration (IDD) has become increasingly apparent due to a growing body of research on its pathogenesis. This review summarizes the role of mitophagy in IDD and the therapeutic potential of targeting this process.

Design: This narrative review is divided into three parts: the regulatory mechanisms of mitophagy, the role of mitophagy in IDD, and the applications and prospects of mitophagy for the treatment of IDD.

ML385 promotes ferroptosis and radiotherapy sensitivity by inhibiting the NRF2-SLC7A11 pathway in esophageal squamous cell carcinoma.

Radiotherapy is important in treating esophageal squamous cell carcinoma (ESCC) comprehensively. Resistance to radiotherapy is a prominent factor contributing to treatment failure in patients with ESCC. The objective of this study was to investigate the impact of ML385, an inhibitor of nuclear factor erythroid 2-related factor 2 (NRF2), on the radiosensitivity of ESCC and elucidate its underlying mechanism.

Novel functional carriers based on Spirulina protein for the delivery of curcumin with improved stability and antioxidant efficiency.

The potential of sustainably sourced Spirulina protein (SP), and SP nanoparticles (SPNP) obtained by heating gelation and ultrasound treatment as functional carriers for curcumin (Cur) was studied. The antioxidant activity, encapsulation performance, stability and digestive properties of the delivery system were analyzed. Compared with SP, SPNP exhibited higher hydrophobicity, and its antioxidant capacity was enhanced due to increased exposure of chromophores.

Effect of Huoxue Huayu decoction on limb swelling in the late stage of bone injury and its influence on motor function.

To explore the therapeutic effect of Huoxue Huayu Decoction on limb swelling in the late stage of bone injury and its influence on motor function. Mainstream databases were searched by inputting the keywords "Huoxue Huayu Decoction" and "bone injury swelling". According to the research scope, 13 articles with a total of 1414 patients were screened out and divided into an observation group (n=707 cases) and a control group (n=707).

Nutritional literacy of patients who underwent surgery for esophageal cancer based on Nutbeam's health literacy model: a qualitative study.

Purpose: The level of nutritional literacy reflects the self-management ability of individuals for a healthy diet and serves as a foundation for effective nutritional interventions. This qualitative study aimed to gain a comprehensive insight into the nutritional literacy levels of patients with esophageal cancer who underwent surgery, obtaining information from both patients and medical staff through interviews.

Methods: Based on Nutbeam's health literacy model, 27 participants were selected from December 2023 to February 2024.

PD-L1 neutrophils induced NETs in malignant ascites is a potential biomarker in HCC.

Background: Since differentiating malignant ascites from benign ascites has always been a clinical difficult, recognition of novel biomarkers in malignant ascites of hepatocellular carcinoma (HCC) patients could be helpful for establishing a diagnosis for HCC patients with ascitic fluids.

Methods: Thirty-five HCC patients with malignant ascites and chronic liver diseases patients with benign ascites were enrolled. Serum and ascites specimens were collected to determine TAN subpopulations and NETs concentration.

A nomogram for enhanced risk stratification for predicting cervical lymph node metastasis in papillary thyroid carcinoma patients.

Background: Cervical lymph node metastasis (CLNM) significantly impacts the prognosis of papillary thyroid carcinoma (PTC) patients. Accurate CLNM prediction is crucial for surgical planning and patient outcomes. This study aimed to develop and validate a nomogram-based risk stratification system to predict CLNM in PTC patients.