Sesquiterpenoids from Inula britannica and their potential mechanism for immunomodulation.

The immune system serves as a role of diseases, such as Parkinson's disease, and acute lung injury. An immunoregulatory activity-directed separation depended on phorbol 12-myristate 13-acetate (PMA) plus ionomycin (Ion)-mediated Jurkat leukemic T cells was used for studying chemical constituents from Inula britannica L. in depth.

Allosteric regulation of Keap1 by 8-hydroxy--cyclocostunolide for the treatment of acute lung injury.

Image 1.

Aucklandiae radix targeted PKM2 to alleviate ulcerative colitis: Insights from the photocrosslinking target fishing technique.

Background: Inflammatory bowel disease (IBD) is a chronic and relapsing disease marked by chronic tissue inflammation that alters the integrity and function of the gut, seriously impacting patient health and quality of life. Aucklandiae Radix (AR), known as Mu Xiang in Chinese, is a traditional Chinese medicine documented in Chinese Pharmacopoeia with effects of strengthening the intestine and stopping diarrhea. However, the potential of AR in treating intestinal inflammation and its underlying mechanism have yet to be further elucidated.

[The Role and Mechanism of MiR-451 in Multidrug Resistance of Leukemia Cell Line K562/A02].

Objective: To detect the differential expressions of miR-451, and in drug-sensitive leukemia cell line K562 and drug-resistant cell line K562/A02, and explore the regulatory relationship between miR-451 and the expressions of and , and the mechanism of miR-451 involved in drug resistance in leukemia.

Methods: CCK-8 assay was used to detect the drug resistance of K562/A02 and K562 cells. Quantitative Real-time PCR (qRT-PCR) was used to verify the differential expressions of miR-451 in K562 and K562/A02 cells.

The association of demodex infestation with pediatric chalazia.

Purpose: This study aimed to investigate the association of Demodex infestation with pediatric chalazia.

Methods: In a prospective study, 446 children with chalazia and 50 children with non-inflammatory eye disease (controls) who underwent surgical treatment were enrolled from December 2018 to December 2019. Patient ages ranged from 7 months to 13 years old.

A Deep Learning Based Framework for Diagnosing Multiple Skin Diseases in a Clinical Environment.

Numerous studies have attempted to apply artificial intelligence (AI) in the dermatological field, mainly on the classification and segmentation of various dermatoses. However, researches under real clinical settings are scarce. This study was aimed to construct a novel framework based on deep learning trained by a dataset that represented the real clinical environment in a tertiary class hospital in China, for better adaptation of the AI application in clinical practice among Asian patients.

The Feature Ambiguity Mitigate Operator model helps improve bone fracture detection on X-ray radiograph.

This study was performed to propose a method, the Feature Ambiguity Mitigate Operator (FAMO) model, to mitigate feature ambiguity in bone fracture detection on radiographs of various body parts. A total of 9040 radiographic studies were extracted. These images were classified into several body part types including 1651 hand, 1302 wrist, 406 elbow, 696 shoulder, 1580 pelvic, 948 knee, 1180 ankle, and 1277 foot images.

Activating transcription factor 3 coordinates differentiation of cardiac and hematopoietic progenitors by regulating glucose metabolism.

The cardiac and hematopoietic progenitors (CPs and HPs, respectively) in the mesoderm ultimately form a well-organized circulation system, but mechanisms that reconcile their development remain elusive. We found that activating transcription factor 3 () was highly expressed in the CPs, HPs, and mesoderm, in zebrafish. The mutants exhibited atrial dilated cardiomyopathy and a high ratio of immature myeloid cells.

[Emission Characteristics of IVOCs from the Combustion of Residential Solid Fuels and the Impact of Burning Temperature].

Intermediate-volatility organic compounds (IVOCs) are important precursors of secondary organic aerosols that have received much recent attention; however, there is a lack of emission data for IVOCs from combustion sources, especially for residential solid fuel combustion. In this study, two bituminous coals with different geological maturity (XZ and LW) and two biomass types (rice stalk and pinewood) were tested at seven temperature points (300-900℃ with an interval of 100℃) using a quartz tube furnace together with a dilution sampling system. The results showed that the averaged emission factor for IVOCs from biomass combustion[(483±182) mg·kg] was 2.

Crystal Structure of GH49 Dextranase from Arthrobacter oxidans KQ11: Identification of Catalytic Base and Improvement of Thermostability Using Semirational Design Based on B-Factors.

The crystal structure of Dextranase from the marine bacterium Arthrobacter oxidans KQ11 (Aodex) was determined at a resolution of 1.4 Å. The crystal structure of the conserved Aodex fragment (Ala52-Thr638) consisted of an N-terminal domain N and a C-terminal domain C.

The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors.

Vasculogenic defects of great vessels (GVs) are a major cause of congenital cardiovascular diseases. However, genetic regulators of endothelial precursors in GV vasculogenesis remain largely unknown. Here we show that Stat4, a transcription factor known for its regulatory role of pro-inflammatory signalling, promotes GV vasculogenesis in zebrafish.

[Multi-spectroscopy applied to study on a late neolithic colored stone from Yuhui Site in Huaihe Basin].

The unearthing of a large number of red-yellow colored stones in Yuhui Site, Bengbu city, Anhui province of China, which was the only site of Longshan culture appointed by "Origin of the Chinese Civilization Project" in the Huaihe River basin, has drawn a widespread attention in archaeology. The present research was undertaken to examine elements, mineral composition and thermal history of one red-yellow color stone in different positions, classified according to specialized colors, by means of synchrotron radiation X-ray fluorescence (SR-XRF), X-ray diffraction (XRD), laser Raman spectroscopy (LRS), electron probe micro-analysis (EPMA) and thermoluminescence (TL). The results show that the main body of the color stone is limonite with a large amount of quartz inclusion.

[Progress and application of zebrafish in regenerative medicine].

The phenomenon of "tissue regeneration" has attracted numerous biologists for many years. Regenerative capacity differs greatly across species. The lower vertebrates such as zebrafish have exceptionally high regeneration abilities, while most high vertebrate species including humans do not have a remarkable ability for regeneration.

[Ambient levels of carbonyl compounds in Shanghai, China].

A ternary-mobile phase program with acetonitrile, water and tetrahydrofuran was developed to identify and quantify 22 carbonyl compounds species by HPLC-UV. The analytical method succeeded in separating acrolein from acetone. Two sampling sites (industrial district and business district) were chosen to study carbonyl compounds levels in ambient air of Shanghai.

Microscale analysis of in vitro anaerobic degradation of lignocellulosic wastes by rumen microorganisms.

Anaerobic degradation of lignin in waste straw by ruminal microbes was directly observed using atomic force microscope (AFM). A series of high-resolution AFM images of the straw surface in the biodegradation show that the wax flakelets and lignin granules covering the straw surface were removed by the rumen microorganisms. Such degradation resulted in an exposure of cellulose fibers located inside the straw.

[Role of pancreatic hilar vascular occlusion in sophisticated pancreaticoduodenectomy].

Objective: To discuss the value of a simple occlusive technique of the triple vessels, ie, portal vein, superior mesenteric vessels and splenic vein, in complicated pancreaticoduodenectomy.

Methods: The technique was fulfilled with a No.8 urethral catheter to encycle the portal vein, superior mesenteric vessels and its near tissue plus pancreatic tail and splenic vein than the neck of pancreas was transected and well exposure superior mesenteric vein and complete transaction of uncinate.

Biodegradable polycation and plasmid DNA multilayer film for prolonged gene delivery to mouse osteoblasts.

Sustained release of functional plasmid DNA from the surfaces of materials which support cell adhesion for tissue formation could have a significant impact on gene therapy and tissue engineering. We report here layer-by-layer assembled multilayer film from a degradable cationic poly(2-aminoethyl propylene phosphate) and plasmid DNA encoding for enhanced green fluorescent protein (EGFP) for mouse osteoblast cell adhesion and prolonged gene delivery. Multilayer film growth was monitored by UV spectrophotometry and intensity of absorbance at 260 nm related to incorporated DNA increased in an exponential manner with increase the number of deposited polymer and plasmid layers.

[Stable carbon isotope analysis method for the atmospheric formaldehyde].

A method of compound-specific stable carbon isotopic analysis for the research about the sources of atmospheric formaldehyde was preliminary studied using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) via 2, 4-dinitrophenylhydrazine (DNPH) derivatization. In order to evaluate the reproducibility, the accuracy and the carbon isotope effects of the method, formaldehyde with different delta 13C values were used to simulate the sampling procedure. The results show that the maximal analytical deviation for all formaldehyde 2, 4-dinitrophenylhydrazone is 0.