Proteomic Identification and Functional Analysis of Reveals Heparin-Binding Proteins.

Glycosaminoglycan (GAG) molecules on the surface of red blood cells play an important regulatory role in the invasion of merozoites of apicomplexan protozoa. Heparan sulfate, a type of GAG molecule, has been identified as an important receptor facilitating the invasion of red blood cells by these parasites. Proteins in the parasite that exhibit strong affinity for heparin may play a pivotal role in this invasion process.

Symptomatic and Asymptomatic Protist Infections in Hospital Inpatients in Southwestern China.

spp., , , and sp. infections have been frequently reported as etiological agents for gastroenteritis, but also as common gut inhabitants in apparently healthy individuals.

Molecular Characterization and Immunological Evaluation of Truncated Rhoptry Neck Protein 2 as a Vaccine Candidate.

is a protozoan that infects red blood cells. Babesiosis is becoming a new global threat impacting human health. Rhoptry neck proteins (RONs) are proteins located at the neck of the rhoptry and studies indicate that these proteins play an important role in the process of red blood cell invasion.

Prevalence and risk factors of Fascioliasis in China.

Fascioliasis has emerged as a significant public health problem among ruminants and humans. Human fascioliasis is a neglected food-borne parasitic disease, which has emerged or reemerged in more than 60 countries worldwide. In China, the first case of human fascioliasis was reported in 1921 in Fujian Province.

[Dynamics of routine blood tests in BALB/c mice with infection].

Objective: To understand the changes in body weight, spleen weight and complete blood cells in BALB/c mice infected with .

Methods: For the infection group, six weeks old BALB/c mice were injected intraperitoneally with a dose of 100 μL of infected blood (20% RBC infection rate, each mouse). For the determination of the progression of infection up to 28 days of the infection, the microscopic visualization of thin blood smears of tail blood stained with Giemsa staining was performed in the infection group.

Urgent needs in fostering neglected tropical diseases (NTDs) laboratory capacity in WHO Western Pacific Region: results from the external quality assessment on NTDs diagnosis in 2012-2015.

Background: Neglected tropical diseases (NTDs) are a heterogeneous group of mainly chronic, debilitating and often stigmatizing diseases that largely affects low-income and politically marginalized populations, causing a large burden of public health, social and economies in the NTDs endemic countries. NTDs are caused by infections with a range of pathogen, including bacteria, parasites, protozoa and viruses. The accurate diagnosis of NTDs is important for reducing morbidity, preventing mortality and for monitoring of control programs.

[Infection of Giardia lamblia in HIV-Infected Individuals and in Kindergarden Children in Rural Area of Anhui and Genotype Analysis].

Objective: To understand the situation of Giardia lamblia infection in HIV-infected individuals and in kindergarden children in rural area of Anhui Province and analyze the genotype of the parasite.

Methods: HIV-infected individuals registered in an AIDS treatment facility and children in a local kindergarden were included in this study during April 24 and May 9, 2015. The feces were collected, stained by iodine solution, and examined by microscopy.

[Observation on growth status of Blastocystis hominis in three media].

Objective: To observe the growth situation of Blastocystis hominis in vitro and select the optimal method for cultivation of B. hominis in different media.

Methods: Ten positive stools with B.

[Establishment of animal model for Pneumocystis carinii and study on etiological and molecular biological detection technology].

Objective: To establish an animal model for Pneumocystis pneumonia (PCP) and to study the etiological and molecular biological technology for PCP detection.

Methods: SD and Wistar rats were divided into experimental and control groups randomly. The animals in the experimental group were immunosuppressed by subcutaneous injection with dexamethasone 2 mg per time per rat, twice a week, while those in the control group underwent the same way of injection with physiological saline simultaneously.

[Establishment of A1E3 and B1C4 monoclonal antibody-based ELISA for detecting circulating antigen of Schistosoma japonicum and its preliminary application].

Objective: To establish A1E3 and B1C4 monoclonal antibody-based ELISA for detecting circulating antigen of Schistosoma japonicum and explore its application value in the field.

Methods: The characteristics of A1E3 and B1C4 monoclonal antibodies were analyzed by SDS-PAGE and Western blotting. The SEA-based ELISA was used to evaluate the titers of A1E3 and B1C4.

Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China.

Background: Schistosomiasis remains a serious public health problem in affected countries, and routine, highly sensitive and cost-effective diagnostic methods are lacking. We evaluated two immunodiagnostic techniques for the detection of Schistosoma japonicum infections: circulating antibody and circulating antigen assays.

Methods: A total of 1864 individuals (between 6 and 72 years old) residing in five administrative villages in Hubei province were screened by serum examination with an indirect hemagglutination assay (IHA).

[Effect comparison between two ELISA kits in IgG antibody detection of Echinococcus granulosus].

Objective: To compare the effects of two ELISA kits on IgG antibody detection of human Echinococcus granulosus.

Methods: A Total of 134 sera of patients with echinococcosis, paragonimiasis westermani, clonorchiasis sinensis, schistosomiasis japonica, and cysticercosis cellulosae, and normal persons were detected by two IgG ELISA kits produced by different companies. Furthermore, the specificity, sensitivity and cross reactivity were counted and analyzed statistically.

[Effect evaluation of three ELISA kits in detection of fasciolasis].

Objective: To evaluate the effect of 3 ELISA kits on detection of human fasciolasis.

Methods: Twenty-six serum samples from patients with fasciolasis, 180 serum samples from patients with other parasitic diseases as well as 26 serum samples from healthy people were detected by ELISA kits which using soluble antigen of Fasciola gigantica, Fasciola hepatica (Fg-ELISA and Fh-ELISA) as well as IgG antigen ELISA detection kits made by DRG company in Germany. The effects of the 3 kits were evaluated.

[Field evaluation of IgG antibody detection kits of Schistosoma japonicum and quality control analysis].

Objective: To evaluate the application effect of an ELISA kit for detection of IgG antibody to Schistosomajaponicum in endemic areas.

Methods: In two schistosomiasis endemic villages of Eryuan County, Yunnan Province, there were 505 permanent residents enrolled in the study. Fecal examinations were carried out by using the nylon screen bag-egg concentration method.

[Establishment of the experimental animal model of Babesia microti].

Objective: To establish the experimental animal model for the study of Babesia microti.

Methods: BALB/c mice, immunosuppressive BALB/c mice, SCID mice and NOD-SCID mice were inoculated with B. microti-infected red blood cells (RBC) by intraperitoneal injection respectively.

[Study on the solid state transformation of PET/nano-CaCO3 composites by variable temperature FTIR spectroscopy].

Solid state transformation of crude poly (ethylene terephthalate)(PET) and PET/nano-CaCO3 (MPET) composites were studied by variable temperature FTIR spectroscopy during the heating process from 40 to 250 degrees C. The effects of nanometer calcium carbonate(nano-CaCO3) on the solid state transformation and crystal correlation bands of MPET composites were analyzed by the curves of the ratio of 1 342 and 1 410 cm(-1) absorbency(A1 342/A1 410) with temperature increasing, and together with DSC curves in the same condition. The results showed that the crystallization degrees of crude PET and MPET are obviously different in this condition by adding nano-CaCO3 particles as inhomogeneous nucleating agents.

[Study on the peaks linked to the crystalline of L-LA with variable temperature FTIR spectra method].

Variable temperature FTIR spectra method was used to study the crystalline spectra of crystal compounds. In the present paper, the sample of L-LA was synthesized and characterized by FTIR, NMR and DSC. It was found that the two peaks in therange of 800-770 cm(-1) changed sharply at 95 degrees C after being investigated by FTIR spectra between 15-120 degrees C.