Capsid protein of turnip crinkle virus suppresses antiviral RNA decay by degrading Arabidopsis Dcp1 via ubiquitination pathway.

RNA decay is a pervasive process in eukaryotic cells. Viruses utilize the host cell's intracellular machinery to gain access to essential molecules and subcellular structures required for infection during the pathogenesis process. The study demonstrates that turnip crinkle virus (TCV) infection enhances the expression of Arabidopsis Dcp1 (AtDcp1), which negatively regulates the accumulation of TCV RNA, indicating its involvement in antiviral defense.

Visualization of mitochondrial molecular dynamics during mitophagy process by label-free surface-enhanced Raman scattering spectroscopy.

Background: Mitophagy is a selective way to eliminate dysfunctional mitochondria and recycle their constituents, which plays an important role in regulating and maintaining intracellular homeostasis. Real-time monitoring mitophagy process is of great importance for cellular physiological and pathological processes related to mitochondria. Howbeit, most of the current methods only focus on single-parameter detection of mitochondrial microenvironmental changes such as pH, viscosity and polarity.

Alleviation effect of macrophage depletion on hepatotoxicity of triptolide: A new insight based on metabolomics and proteomics.

Ethnopharmacological Relevance: Triptolide (TP) is an abietane-type diterpenoid isolated from the traditional Chinese herb Tripterygium wilfordii Hook. F, which is used to relieve rheumatism, alleviate joint pain and swelling, and promote blood circulation for more than 600 years in China. The most common preparations containing TP from Tripterygium wilfordii Hook F, which are Tripterygium tablets and Tripterygium glycoside tablets, are widely used in clinical for treating rheumatoid arthritis and other autoimmune diseases at present.

Microstructural white matter injury contributes to cognitive decline: Besides amyloid and tau.

Background: Cognitive decline and the progression to Alzheimer's disease (AD) are traditionally associated with amyloid-beta (Aβ) and tau pathologies. This study aims to evaluate the relationships between microstructural white matter injury, cognitive decline and AD core biomarkers.

Methods: We conducted a longitudinal study of 566 participants using peak width of skeletonized mean diffusivity (PSMD) to quantify microstructural white matter injury.

Uncommon Diterpenoids with Diverse Frameworks from the South China Sea Sponge and Their Anti-inflammatory Activities.

Uncommon diterpenoids with diverse frameworks, including one unexpected iodinated oxa-6/6/6/6-tetracyclic diterpene () and its monobrominated 6/6/6-tricyclic analogue () and one novel isodolastane-type diterpene featuring an unusual aromatic 5/7/6-tricyclic ring system () as well as a related known dolastane-type diterpenoid (), were isolated from the South China Sea sponge . Their structures, including absolute configurations, were established by extensive spectroscopic data analysis, X-ray diffraction analysis, and quantum mechanical-nuclear magnetic resonance and time-dependent density functional theory/electronic circular dichroism calculations. A plausible biosynthetic pathway of new compounds - was proposed.

Ascaris lumbricoides a rare cause gastric perforation: a case report and brief literature review.

(AL), a prevalent nematode causing ascariasis, infects millions worldwide, with a higher risk in preschool and school-aged children. Though infections are usually mild, rare and life-threatening complications like gastrointestinal perforation exist. This article documents a case involving a 61-year-old deaf-mute man who presented with a month-long history of epigastric pain accompanied by nausea, anorexia, and constipation.

Amide proton transfer-weighted (APTw) imaging and derived quantitative metrics in evaluating gliomas: Improved performance compared to magnetization transfer ratio asymmetry (MTR).

Rationale And Objectives: Isocitrate dehydrogenase (IDH) status, glioma subtypes and tumor proliferation are important for glioma evaluation. We comprehensively compare the diagnostic performance of amide proton transfer-weighted (APTw) MRI and its related metrics in glioma diagnosis, in the context of the latest classification.

Materials And Methods: Totally 110 patients with adult-type diffuse gliomas underwent APTw imaging.

Variables associated with cognitive function: an exposome-wide and mendelian randomization analysis.

Background: Evidence indicates that cognitive function is influenced by potential environmental factors. We aimed to determine the variables influencing cognitive function.

Methods: Our study included 164,463 non-demented adults (89,644 [54.

Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.

Humans are widely exposed to phthalates, a common chemical plasticizer. Previous cohort studies have revealed that maternal exposure to monobutyl phthalate (MBP), a key metabolite of phthalates, is associated with neurodevelopmental defects. However, the molecular mechanism remains unclear.

Epilepsy and brain health: a large prospective cohort study.

Background: Epilepsy, as a chronic noncommunicable disease with recurrent seizures, may be a marker of deterioration or alteration in other underlying neurological diseases. This study aimed to investigate the relationship of epilepsy with brain function, other common brain disorders, and their underlying mechanisms.

Methods: The study was based on clinical diagnostic and test data from 426,527 participants in the UK Biobank, of whom 3,251 were diagnosed with epilepsy at baseline.

Crocodile optimization algorithm for solving real-world optimization problems.

Nature-inspired bionic algorithms have become one of the most fascinating techniques in computational intelligence research, and have shown great potential in real-world challenging problems for their simplicity and flexibility. This paper proposes a novel nature-inspired algorithm, called the crocodile optimization algorithm (COA), which mimics the hunting strategies of crocodiles. In COA, the hunting behavior of crocodiles includes premeditation and waiting hunting.

METTL14 Mediates m6A methylation to improve osteogenesis under oxidative stress condition.

Objectives: Bone remodeling imbalance contributes to osteoporosis. Though current medications enhance osteoblast involvement in bone formation, the underlying pathways remain unclear. This study was aimed to explore the pathways involved in bone formation by osteoblasts, we investigate the protective role of glycolysis and N6-methyladenosine methylation (m6A) against oxidative stress-induced impairment of osteogenesis in MC3T3-E1 cells.

Ensemble learning-based radiomics model for discriminating brain metastasis from glioblastoma.

Objective: Differentiating between brain metastasis (BM) and glioblastoma (GBM) preoperatively is challenging due to their similar imaging features on conventional brain MRI. This study aimed to enhance diagnostic accuracy through a machine learning model based on MRI radiomics data.

Methods: This retrospective study included 235 patients with confirmed solitary BM and 273 patients with GBM.

Dual-objective optimization of prefabricated component logistics based on JIT strategy.

Prefabricated construction involves manufacturing components in a factory and then transporting them to a construction site for assembly, yielding resource savings and improved efficiency. However, the large size and weight of prefabricated components, along with strict delivery requirements, introduce logistical challenges, such as increased carbon emissions during transport and site congestion. This study addresses the dual-objective vehicle scheduling problem for prefabricated components.

A multifunctional hyaluronic acid-engineered mesoporous nanoreactor with HO/O self-sufficiency for pH-triggered endo-lysosomal escape and synergetic cancer therapy.

Monotherapy has poor accuracy and is easily restricted by tumor microenvironment (TME). Remodeling components of the TME to activate multimodal cancer therapy with high precision and efficiency is worth exploring. A multifunctional nanoreactor was fabricated by decorating chlorin e6-modified and PEGylated hyaluronic acid bearing diethylenetriamine-conjugated dihydrolipoic acid on the surface of glucose oxidase (GOx)-loaded hollow mesoporous CuS nanoparticles (labeled as GOx@HCuS@HA).

A prospective, self-controlled study of sub-plateau heart rate variability in healthy adults.

Background: The low-pressure, hypoxic environment characteristic of high-altitude regions significantly affects the cardiovascular and autonomic nervous system functions of individuals, consequently impairing their sleep quality. Heart rate variability, a non-invasive indicator of autonomic nervous system activity and balance within the cardiovascular system, has not been thoroughly investigated in terms of its patterns during acclimatization and de-acclimatization phases for individuals traveling to and residing in high-altitude areas and its relationship with sleep stability.

Methods: Data was collected from 22 medical staff members who traveled from Chengdu to Yecheng, with measurements taken before leaving Chengdu, 1 week in Yecheng, 3 months in Yecheng, and 1 week after returning to Chengdu.

Site-Selective Electrophilic Trifluoromethylthiolation for the Synthesis of C5- or C7-SCF-Substituted Indolines.

We report herein an efficient and site-selective electrophilic trifluoromethylthiolation of indolines. In the absence of any catalyst or additive, C5-selective trifluoromethylthiolation could proceed at room temperature. With palladium used as the catalyst, the selectivity was reversed completely, giving C7-selecive trifluoromethylthiolated products.

Therapeutic endoscopic retrograde cholangiopancreatography in a patient with asplenia-type heterotaxy syndrome: A case report.

Background: Asplenia-type heterotaxy syndrome (HS) is rare and refers to visceral malposition and dysmorphism. It is associated with a high infant mortality rate due to cardiac anomalies, and related digestive endoscopic interventions are poorly understood. With the improved long-term prognosis of these individuals after modern cardiac surgery, intra-abdominal anomalies have become increasingly significant.

Discovery of Ureido-Containing Alteropeptilides and Intramolecular Cyclized Alteramides in S16 by Promoter Engineering of Cryptic Biosynthetic Gene Clusters.

are abundant in the oceans and possess great potential in the synthesis of bioactive natural products. Although many secondary metabolite biosynthetic gene clusters have been identified from genomes, most of their products have not been characterized. In this study, endogenous constitutive promoters with high transcriptional activity were obtained from S16 through RNA-seq and a fluorescence assay of luciferase gene expression.