Publications by authors named "Yamilka Lago-Alvarez"
Article Synopsis
- Current methods for detecting pathogens rely mainly on fungal and bacterial cultures, but next-generation sequencing (NGS) shows promise for identifying microbial DNA in equine uterine samples.
- In the study, there was an 80% agreement for fungal cultures and a 74% agreement for bacterial cultures when compared to NGS, indicating a moderate correlation between the two methods.
- Overall, next-generation sequencing could serve as a valuable diagnostic tool for better detecting pathogens in equine endometritis, complementing traditional culture methods.
This study aimed to describe and compare semen parameters (pre-freeze and post-freezing) and antisperm antibodies (ASA) of donkeys with epididymal sperm granuloma and healthy controls. Feral donkeys (n = 10) castrated in a concurrent study were enrolled in the present experiment. Three donkeys had unilateral granulomas, two donkeys had bilateral granulomas, whereas the remaining five had grossly normal epididymides.
View Article and Find Full Text PDFThe objectives of this study were to assess the cooling and freezing of donkey epididymal semen harvested immediately after castration (Experiment 1, = 4) or after the shipment (24 or 48 h) of epididymides attached to testicles (Experiment 2, = 14) or dissected apart (Experiment 3, = 36). In each experiment, semen was frozen immediately (Non-Centrif) in an egg yolk-based semen extender (EY) or after processing through cushion-centrifugation (Centrif) while extended in a skim milk-based extender (SC). In all three experiments, cooled, pre-freeze, and post-thaw epididymal semen was assessed for total motility (TM), progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP).
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