Optical microscopic study on a novel morphological classification method of multiple diagnostic features of var. .

Optical microscopy is the gold standard technique used to confirm the diagnosis of scabies. Multiple diagnostic features of the pathogen var. () can be identified under a microscope and classified into 3 categories: mites, eggs and fecal pellets.

Mechanism of protection from insulin resistance by toll-like receptor 2 deficiency in high-fat diet fed mice: involvement in macrophage polarization.

Background: Toll-like receptor 2 (TLR2) deficiency can increase insulin sensitivity and improves glucose tolerance. However, it is not yet fully understood about its underlying mechanism. The regulation of M1/M2 macrophage polarization has been verified to involve in insulin resistance.

[Knockout of TLR2 gene attenuates insulin resistance and promotes M2 polarization of macrophages in mice].

Objective To study the effect of deletion of Toll-like receptor 2 (TLR2) gene on insulin resistance and polarization of macrophages in mice. Methods The wild-type (WT) and TLR2 knockout (TLR2) C57BL/6 male mice, aged 28 days, were selected, with 12 mice in each group. The genotype of each mouse was identified by PCR.

[Identification and bioinformatics analysis of differentially expressed microRNAs in mice liver tissues with a high fat diet-induced insulin resistance].

Objective: To identify the expression levels of miR-1897-3p, miR-690 and miR-7a-5p in mice liver tissues with a high fat diet-induced insulin resistance using a stem-loop reverse transcriptional real-time fluorescence quantitative PCR( RT-q PCR)method, and predict microRNAs( miRNAs)-regulated target genes and their functions to investigate the relationship between insulin resistance and differentially expressed miRNAs.

Methods: The total of 30 liver tissue samples were obtained from 15 normal control mice and 15 test mice with a high fat diet-induced insulin resistance. The stemloop reverse transcriptional primers and the primers of real-time PCR were designed to establish a RT-q PCR method, and then the expression levels of miR-1897-3p, miR-690 and miR-7a-5p of 30 liver tissue samples were detected and analyzed.