The Immunologic Profiles of Kawasaki Disease Triggered by Mycoplasma pneumoniae Infection.

Objective: We compared the immunologic characteristics of mycoplasma pneumoniae-triggered Kawasaki disease (MP-KD) with Kawasaki disease (KD) not associated with mycoplasma pneumoniae (MP), with mycoplasma pneumoniae-triggered Henoch-Schönlein purpura (MP-HSP), and with healthy controls.

Methods: Complement levels, cellular and humoral immunity were assessed in KD, in MP-KD, in MP-HSP, and in healthy children.

Results: Of 622 children with KD, 74 had MP-KD.

Development of a reliable UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in various feed products.

A reliable ultra-high-performance liquid chromatography-tandem mass spectrometry method (UHPLC-MS/MS) was developed for the simultaneous determination of two mycotoxins, that is, zearalenone (ZEN) and zearalenone-14-glucoside (ZEN-14G) in formula feed, concentrated feed, and premixed feed products. An improved sample pretreatment was achieved with the hydrophilic-lipophilic balance (HLB) cartridges efficiently removing the impurities and enriching the target analytes in different feeds. The critical parameters affecting the performance of the solid-phase extraction (SPE) procedure were carefully optimized, and 20% acetonitrile in water as the loading solution, 50% methanol in water as the washing solvent, and 5 ml of methanol as the elution solvent yielded the optimal purification efficiencies.

Genotyping-by-Sequencing of Races and Cultivars Uncovers Novel Patterns of Genetic Relationships and Domestication Footprints.

Determining the genetic rearrangement and domestication footprints in cultivars and primitive race genotypes are essential for effective gene conservation efforts and the development of advanced breeding molecular markers for marker-assisted breeding. In this study, 94 accessions representing the 7 primitive races of , along with 9 and 12 cultivated accessions were evaluated. The genotyping-by-sequencing (GBS) approach was employed and 146 558 single nucleotide polymorphisms (SNP) were generated.

An effective identification and quantification method for Ginkgo biloba flavonol glycosides with targeted evaluation of adulterated products.

Background: Ginkgo biloba L. (Ginkgoaceae) leaf extract is one of the most popular herbal products on the market, as it contains flavone glycosides (≥ 24%) and terpene lactones (≥ 6%), which are proposed to have significant physiological effects. Unfortunately, the challenging financial climate has resulted in a natural health product market containing adulterated ginkgo products.

Anti-tumor and anti-angiogenic effects of Macrothelypteris viridifrons and its constituents by HPLC-DAD/MS analysis.

Ethnopharmacological Relevance: Macrothelypteris viridifrons is widely distributed in south of China and has been used as folk medicine to treat cancer, hydropsy, and traumatic bleeding.

Aim Of The Study: To investigate the chemical constituents and the anti-tumor and anti-angiogenic effects of Macrothelypteris viridifrons.

Materials And Methods: An HPLC-DAD/MS technique was used to determine the flavonoid profile of Macrothelypteris viridifrons.

Nephroprotective activity of Macrothelypteris oligophlebia rhizomes ethanol extract.

Context: Macrothelypteris oligophlebia (Bak.) Ching (Thelypteridaceae) is a Chinese herbal medicine used traditionally for the treatment of diseases such as edema, boils, burns, and roundworms. However, research about the nephroprotective potential of this plant is not available.

Characterisation of phenols and antioxidant and hypolipidaemic activities of Lethariella cladonioides.

Background: This study aimed to investigate the antioxidant and hypolipidaemic activities of an ethanol extract of Lethariella cladonioides (Nyl.) Krog (EE) and to characterise its chemical constituents.

Results: Nine phenols were identified as canarione, thamnolic acid, squamatic acid, vermicularin, norstictic acid, baeomycesis acid, lecanoric acid, barbatinic acid and usnic acid from analysis of EE by using high-performance liquid chromatography with a diode array detector-mass spectrometry.

Topical anti-inflammatory and analgesic activity of kirenol isolated from Siegesbeckia orientalis.

Ethnopharmacological Relevance: Siegesbeckia orientalis has been traditionally used as a topical anti-inflammatory and analgesic agent.

Aims Of The Study: Current study was designed to explore the topical anti-inflammatory and analgesic effects of a constituent isolated from Siegesbeckia orientalis (Compositae), in order to validate its folk use.

Materials And Methods: Kirenol was isolated from ethanolic extract of Siegesbeckia orientalis.

A novel non-aromatic B-ring flavonoid: isolation, structure elucidation and its induction of apoptosis in human colon HT-29 tumor cell via the reactive oxygen species-mitochondrial dysfunction and MAPK activation.

The aim of the present study was to elucidate the chemical structure of a novel non-aromatic B-ring flavonoid (DHEC) isolated from Macrothelypteris viridifrons and to evaluate its putative molecular mechanism of action on induction of apoptosis in human colon HT-29 cancer cell. On the basis of MS, UV, IR, 1D and 2D NMR data, DHEC was identified as 2-(cis-1, 2-dihydroxy-4-oxo-cyclohex-5-enyl)-5-hydroxy-7-ethoxy-chromone. In addition, the cytotoxicity of DHEC and its effect on induction of apoptosis were confirmed by several assays.

[Flavonoids with special B-ring from Macrothelypteris viridifrons and their anti-proliferative effects on tumor cell].

Objective: To study the chemical constituents of Macrothelypteris viridifrons and their anti-proliferative effects on tumor cell.

Method: The compounds were isolated by column chromatography with silica gel, C18 reverse-phase silica gel, sephadex LH-20, and their structures were elucidated on the basis of physiochemical propertities and spectral analysis. The antitumor activities of all compounds were tested with MOLT4, Hep G2, A-549, MCF-7, HT-29, PC-3 tumor cell lines.

In vitro and in vivo evaluation of the wound healing properties of Siegesbeckia pubescens.

Ethnopharmacological Relevance: Siegesbeckia pubescens (SP) has been traditionally used as a wound healing agent.

Aim Of The Study: Investigate in vitro and in vivo healing properties of SP extract.

Materials And Methods: The methanolic extract of SP was tested for the ability to stimulate the growth of mouse fibroblast NIH3T3 in vitro.

Flavonoids from the aerial parts of Macrothelypteris torresiana.

Two new flavone derivatives (1 and 2) were isolated from the aerial parts of Macrothelypteris torresiana, along with four known flavonoids: protoapigenin, apigenin, kaempferol and quercetin. The structures were determined on the basis of spectroscopic data. Compound 1 showed weak cytotoxic activity against human tumour cell lines HepG₂ , MCF₇ and K562.

Parathelypteriside attenuates cognition deficits in d-galactose treated mice by increasing antioxidant capacity and improving long-term potentiation.

Parathelypteriside (PG), a stilbenoid compound, was extracted from Parathelypteris glanduligera (kze.) ching that exhibits antioxidative and anti-inflammatory effects. The aim of this study was to investigate the protective effect of PG against the d-galactose (d-gal)-induced neurotoxicity in mice.

Antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica (Franch. et Sav.) Ching.

Aim Of The Study: The present study was conducted to evaluate the antioxidant, free radical scavenging, hepatoprotective and anti-inflammatory potential of Parathelypteris nipponica (Franch. et Sav.) Ching.

Antioxidant and hepatoprotective activity of ethanol extract of Arachniodes exilis (Hance) Ching.

Aim Of The Study: The study was aimed to investigate the ethanol extract of Arachniodes exilis for the antioxidant and hepatoprotective activity.

Materials And Methods: Antioxidant activity was evaluated by different assays, including reducing power, lipid peroxidation, 2, 2'-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), superoxide anion, hydroxyl radicals and hydrogen peroxide. The hepatoprotective activity of ethanol extract was studied on mice liver damage induced by CCL(4) by monitoring biochemical parameters.

Bis(2,6-dihy-droxy-benzoato-κO,O)(nitrato-κO,O')bis-(1,10-phenanthroline-κN,N')dysprosium(III).

In the mononuclear title complex, [Dy(C(7)H(5)O(4))(2)(NO(3))(C(12)H(8)N(2))(2)], the Dy(III) atom is in a distorted bicapped square-anti-prismatic geometry formed by four N atoms from two chelating 1,10-phenanthroline (phen) ligands, four O atoms from two 2,6-dihy-droxy-benzoate (DHB) ligands and two O atoms from a nitrate anion. Inter-molecular π-π stacking inter-actions between the phen and DHB ligands [centroid-centroid distances = 3.542 (4) and 3.

Diaqua-(2,6-dihy-droxy-benzoato-κO,O)bis-(2,6-dihy-droxy-benzoato-κO)bis-(1,10-phenanthroline-κN,N')lanthanum(III)-1,10-phenanthroline (1/1).

In the title compound, [La(C(7)H(5)O(4))(3)(C(12)H(8)N(2))(3)(H(2)O)(2)]·C(12)H(8)N(2), the La(III) atom is coordinated by four N atoms from two chelating 1,10-phenanthroline (phen) ligands, four O atoms from three 2,6-dihy-droxy-benzoate (DHB) anions (one monodentate, the other bidentate) and two water O atoms, completing a distorted LaN(4)O(6) bicapped square-anti-prismatic geometry. Within the mononuclear complex mol-ecule, intra-molecular π-π stacking inter-actions are observed, the first between a coordinated phen mol-ecule and a DHB ligand [centroid-centroid distance = 3.7291 (16) Å], and the second between a coordinated phen mol-ecule and an uncoordinated phen ligand [centroid-centroid distance = 3.

Extraction and determination of some psychotropic drugs in urine samples using dispersive liquid-liquid microextraction followed by high-performance liquid chromatography.

A simple, rapid and sensitive method termed as dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-ultraviolet detector (HPLC-UV) has been proposed for the determination of three psychotropic drugs (amitryptiline, clomipramine and thioridazine) in urine samples. The determination was performed on a C(8) column under the optimal chromatographic conditions (mobile phase: ammonium acetate (0.03 mol L(-1), pH 5.

Development and evaluation of the Sinomenine transdermal patch.

Sinomenine transdermal patch was prepared and its properties were studied. The patches were produced by salivation method. The releasing rate in vitro of the patch was determined by HPLC.

[Two new flavan glycosides from Abacopteris penangiana].

To study the chemical constituents of Abacopteris penangiana (Hook.) Ching, various chromatographic techniques were used to isolate and purify the constituents. The structures of the obtained compounds were elucidated by spectroscopic data and physical-chemical properties.

[Phenols from the rhizomes of Arachniodes exilis].

Objective: To study the chemical constituents of Arachniodes exilis.

Methods: The methanolic extract was isolated and purified by silica gel, C18 reverse phase silica gel and Sepadex LH-20 gel column chromatography. The chemical structures were elucidated on the basis of the physicochemical properties and spectral data.

[Studies on chemical constituents of Arachniodes rhomboidea].

Objective: To study chemical constituents of Arachniodes rhomboidea.

Method: Silica gel column chromatography and Sephadex LH -20 gel column chromatography were employed for the isolation and purification. The structures were identified on the basis of spectral data and chemical methods.