Physiological and transcriptomic analyses reveal tea plant (Camellia sinensis L.) adapts to extreme freezing stress during winter by regulating cell wall structure.

Tea plants grown in high-latitude areas are often damaged by extreme freezing temperatures in winter, leading to huge economic losses. Here, the physiological and gene expression characteristics of two tea cultivars (Xinyang No. 10 (XY10), a freezing-tolerant cultivar and Fudingdabaicha (FDDB), a freezing-sensitive cultivar) during overwintering in northern China were studied to better understand the regulation mechanisms of tea plants in response to natural freezing stress.

: Effects of mobile phone use on semen parameters: a cross-sectional study of 1634 men in China.

Mobile phones play an irreplaceable role in modern people's lives. However, the radiofrequency electromagnetic radiation produced by mobile phones has also caused increasing concern. A cross-sectional study was conducted to investigate the effect of radiofrequency electromagnetic radiation produced by mobile phones on semen parameters in 1634 men who underwent semen examination at the Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, China.

VmMon1-Ccz1 Complex Is Required for Conidiation, Autophagy, and Virulence in .

Apple Valsa canker caused by is a serious disease in eastern Asia, especially in China. In our previous proteomics study, monensin sensitivity 1 protein in () was identified to be significantly upregulated during infection. It was reported Mon1 protein formed a heterodimer called MC (Mon1-Ccz1) complex with caffeine, calcium, and zinc sensitivity 1 protein (Ccz1) in yeast.

The clinical application value of phase angle of six parts in nutritional evaluation of tumor patients.

Objectives: The purpose of this study was to explore the clinical application value of phase angle (PA) of six parts in the nutritional evaluation and construct a prediction model for diagnosing malnutrition of tumor patients.

Methods: A total of 1129 patients with malignant tumors were analyzed retrospectively. The age, sex, tumor location and body mass index (BMI) of the patients were collected, and PA of six parts was measured.

The effect of sulfur on the leaching of Cr, Cr, Pb and Zn from fly ash glass.

This work assessed the capture and subsequent release of potentially harmful Cr(VI), Cr(III), Pb(II) and Zn(II) ions in and from dechlorinated fly ash glass. Differential scanning calorimetry, Fourier transform infrared spectroscopy, scanning electron microscopy - energy dispersive spectroscopy and inductively coupled plasma spectrometry along with other analytical techniques were used to explore the mechanism by which sulfur affected the immobilization and long-term leaching behavior of heavy metals in fly ash glass. Working with a CaO-MgO-AlO-SiO-SO system, increasing the sulfur content was found to promote the leaching of Cr but had only a minimal effect on the loss of Pb and Zn.

Effects of mobile phone use on semen parameters: a cross-sectional study of 1634 men in China.

Mobile phones play an irreplaceable role in modern people's lives. However, the radiofrequency electromagnetic radiation produced by mobile phones has also caused increasing concern. A cross-sectional study was conducted to investigate the effect of radiofrequency electromagnetic radiation produced by mobile phones on semen parameters in 1634 men who underwent semen examination at the Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, China.

Biological evidences for successive oogenesis and egg-laying of Matsumurasca onukii.

Tea plant (Camellia sinensis) is one of the most important horticultural cash crops, and tea green leafhopper (Matsumurasca onukii) is an extremely harmful sap-sucking pest of tea plant. Serious generation overlapping, which is mainly caused by the long oviposition period, leads to poor control effect of pesticides on this pest in the tea plantation. But the intuitive evidences of continuous oogenesis and egg-laying of this pest are still lacking, which seriously hindered the development of genetic control methods.

The performance of water-soluble fluoride transformation in soil-tea-tea infusion chain system and the potential health risk assessment.

Background: Water-soluble fluoride (WS-F) can be absorbed directly by tea plants from soil and comprises a major source of dietary F in tea consumers. To reveal the WS-F accumulation in tea leaves and assess WS-F health risks, 70 sets of samples including tea leaves at three maturity stages and corresponding topsoil were collected from Xinyang, China. The WS-F contents in tea samples and pH values in soil samples were determined.

(+)-Catechin, epicatechin and epigallocatechin gallate are important inducible defensive compounds against Ectropis grisescens in tea plants.

The tea plant, Camellia sinensis (L.) O. Kuntze, is an economically important, perennial woody plant rich in catechins.

Nep1-like Proteins from Differentially Regulate Pathogen Virulence and Response to Abiotic Stresses.

Necrosis and ethylene-inducing peptide 1(Nep1)-like protein (NLP) is well known for its cytotoxicity and immunogenicity on dicotyledonous, and it has attracted large attention due to its gene expansion and functional diversification in numerous phytopathogens. Here, two NLP family proteins, VmNLP1 and VmNLP2, were identified in the pathogenic fungus . We showed that VmNLP2 but not VmNLP1 induced cell death when transiently expressed in .

The comparison of economic impacts of COVID-19 between China and the United States.

The outbreak of the COVID-19 in 2020 has a severe impact on all countries. This paper first studies the impact of the epidemic on the macro-economy in China, which is one of the countries with early epidemic situation, in the rapid development stage of the epidemic situation, and the specific performance in macro-economy aspects. On this basis, study the development trend and macroeconomic performance of the United States as one of the most serious countries, and the relationship between the epidemic development and macro-economy in the United States.

Exploring Factors Associated with Functional Change and Predictors of Participation Improvement-A Two Years Follow-Up on People with Depression.

The purpose of this study is to understand the functional status distribution and to explore the factors associated with changes in functional status and social participation in people with depression using two-year follow-up data. Subjects were selected from the Taiwan Databank of Persons with Disabilities (TDPD) if they had an evaluation date between July 2012 and 31 December 2017. We used data for 1138 individuals with multiple evaluation records and who were diagnosed with depression.

Development and Application of a LAMP Assay for the Detection of the Latent Apple Tree Pathogen .

, the causal agent of apple Valsa canker, produces cankers, resulting in the death of infected tissues and eventually the entire tree. Because of the long latent period of the disease, it is necessary to develop a rapid, sensitive, and reliable field-based assay to effectively diagnose apple Valsa canker when the plant is still symptomless. Loop-mediated isothermal amplification (LAMP) is a novel detection method that synthesizes a large amount of DNA and produces the visible byproduct (magnesium pyrophosphate) without conventional thermal cycling.

Thermal damage width and hemostatic effect of bipolar electrocoagulation, LigaSure, and Ultracision techniques on goat mesenteric vessels and optimal power for bipolar electrocoagulation.

Background: We aimed to determine the optimal bipolar electrocoagulation power for laparoscopic surgery and to investigate which method, bipolar electrocoagulation, advanced bipolar, or ultrasonic technique was more reliable.

Methods: Goat mesenteric vessels (210 in vivo samples) with diameters of 3.03-5.

Serum Interleukin-9 Concentration Is Associated with the Hemoglobin Level and Renal Function in Patients with Multiple Myeloma.

Background: Multiple myeloma (MM) is one of the most common types of hematological malignancies, but its pathogenesis is poorly understood. Interleukin-9 (IL-9) is a growth factor, mainly produced by helper T cells. A series of observations suggested that IL-9 might act as a factor promoting oncogenesis.

[Influence of body fat percentage and body mass index on semen parameters in adult males].

Objective: To explore the influence of body fat percentage (BF%) and body mass index (BMI) on the semen quality of adult males.

Methods: A total of 125 randomly selected male infertility patients underwent examinations of semen quality, BMI and BF% on the day of enrollment. With BMI ≥28 kg/m2 as the criterion of obesity, 50 of the patients fell into the category of obesity and 75 into that of non-obesity, while with BF% >25% as the criterion, 69 belonged to the obesity and 56 to the non-obesity type.

Bone marrow PD-1 positive T cells reflect tumor mass and prognosis in multiple myeloma.

Programmed cell death protein 1 (PD-1) negatively regulates T cell effector mechanisms and contributes to tumor cell escape from immune surveillance. To evaluate potential clinical significance of PD-1 in multiple myeloma (MM), we quantified PD-1 expressing T cells in bone marrow (BM) of MM patients using flow cytometry. Our results showed that PD-1 positive T cells in BM from relapsed/refractory patients were significantly higher than those in the newly diagnosed, partial/complete remission MM, and controls, respectively.

Synthesis of Au nanoparticle-decorated carbon nitride nanorods with plasmon-enhanced photoabsorption and photocatalytic activity for removing various pollutants from water.

Herein we have developed Au nanoparticle-decorated carbon nitride (Au-CN) nanorods as novel and efficient photocatalysts. Au-CN with different Au/CN precursor molar ratios (0.5%, 1% and 2%) have been prepared by a solvothermal-hydrothermal two-step method, where CN nanorods have diameters of 20-30nm and length of 0.

Case detection and diagnosis of primary aldosteronism - The consensus of Taiwan Society of Aldosteronism.

Background/purpose: Even though the increasing clinical recognition of primary aldosteronism (PA) as a public health issue, its heightened risk profiles and the availability of targeted surgical/medical treatment being more understood, consensus in its diagnosis and management based on medical evidence, while recognizing the constraints of our real-world clinical practice in Taiwan, has not been reached.

Methods: The Taiwan Society of Aldosteronism (TSA) Task Force acknowledges the above-mentioned issues and reached this Taiwan PA consensus at its inaugural meeting, in order to provide updated information of internationally acceptable standards, and also to incorporate our local disease characteristics into the management of PA.

Results: When there is suspicion of PA, a plasma aldosterone to renin ratio (ARR) should be obtained initially.

Immunogenicity and safety of subunit plague vaccine: A randomized phase 2a clinical trial.

Background: Although the killed whole-cell and live attenuated plague vaccine have been licensed, they are rarely used today because of toxicities, limited evidence of efficacy against plague, poor immune persistence required booster immunization every year, and limited commercial availability. This study was a randomized phase 2a clinical trial aimed to evaluating the immunogenicity and safety of a novel subunit plague vaccine.

Methods: 240 healthy adults aged 18-55 y were enrolled and randomly assigned at a ratio of 1:1 to receive 2 doses of 15 or 30 mcg vaccine at a 28-day interval between doses.

Laminin 411 acts as a potent inducer of umbilical cord mesenchymal stem cell differentiation into insulin-producing cells.

Background: Diabetes mellitus (DM) is an incurable metabolic disease constituting a major threat to human health. Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) hold great promise in the treatment of DM. The development of an efficient IPC induction system is a crucial step for the clinical application of IPCs for DM.

[Expression of pituitary tumor transforming gene and C-myc gene in patients with multiple myeloma].

The study was purposed to explore the expressions of pituitary tumor transforming gene and c-myc gene in patients with multiple myeloma (MM) and its relationship with pathogenesis of MM. Expressions of pituitary tumor transforming gene and c-myc gene mRNA in BMMNC from 33 patients with MM and 10 normal controls were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that the expressions of pituitary tumor transforming gene and c-myc gene mRNA were significantly higher in MM patients those that in normal controls (p<0.

[Preliminary study of multivariable model in predicting response to immunosuppressive therapy in patients with aplastic anemia].

Objective: To evaluate the potential usefulness of a multivariable model in predicting the response to immunosuppressive therapy (IST) in patients with aplastic anemia (AA), and its application to the clinical practice.

Methods: PB T cells subpopulation and BM T cells intracellular IFN-gamma and IL-4 were serially analyzed by flow cytometry (FCM) before and during treatment. HLA-DRB1 * 1501 phenotype was analyzed by PCR-SSP.