Study on the Properties of All-Solid Waste Fluidized Filling Materials Applied to Mine Void Area Filling Engineering.

The extraction of mining resources, as well as processing processes such as ore beneficiation and smelting, generate large amounts of tailings that are difficult to directly utilize. Meanwhile, substantial filling materials are required for the voids formed after mining operations, and the environmental issues and safety hazards brought on by massive solid waste disposal cannot be ignored. By utilizing solid waste with alkaline and pozzolanic activity as the binder component and gold tailings as filler aggregate to prepare filler material to fill up the void areas, the purpose of waste treatment can be achieved.

Durability Performance of CGF Stone Waste Road Base Materials under Dry-Wet and Freeze-Thaw Cycles.

The disposal of stone waste derived from the stone industry is a worldwide problem. The shortage of landfills, as well as transport costs and environmental pollution, pose a crucial problem. Additionally, as a substitute for cement that has high carbon emissions, energy consumption, and pollution, the disposal of stone wastes by utilizing solid waste-based binders as road base materials can achieve the goal of "waste for waste".

Mechanical properties and microscopic mechanism of solid waste based binder solidified stone waste.

The stone waste generated by stone industry occupy land resources, cause safety hazards and need to be efficiently resourcefully utilized. In this study, the CGF solid waste based binder (abbreviated as CGF) with calcium carbide residue (CCR), ground granulated blast furnace slag (GGBS), and fly ash (FA) as components was developed to solidify the stone waste. Through "treating waste with waste", the resource utilization of solid waste was realized.

Effect of Fiber Content on Mechanical Properties of Fiber-Reinforced CGF All-Solid-Waste Binder-Solidified Soil.

In order to realize the resource utilization of solid waste and improve the tensile strength and toughness of soil, CCR-GGBS-FA all-solid-waste binder (CGF) composed of general industrial solid waste calcium carbide residue (CCR), ground granulated blast furnace slag (GGBS) and fly ash (FA) was used instead of cement and combined with polypropylene fiber to strengthen the silty soil taken from Dongying City, China. An unconfined compressive strength test (UCS test) and a uniaxial tensile test (UT test) were carried out on 10 groups of samples with five different fiber contents to uncover the effect of fiber content on tensile and compressive properties, and the reinforcement mechanism was studied using a scanning electron microscopy (SEM) test. The test results show that the unconfined compressive strength, the uniaxial tensile strength, the deformation modulus, the tensile modulus, the fracture energy and the residual strength of fiber-reinforced CGF-solidified soil are significantly improved compared with nonfiber-solidified soil.

deficiency promotes lung cancer metastasis by activating Notch signaling in cancer-associated fibroblasts.

Lung adenocarcinoma, the most prevalent lung cancer subtype, is characterized by its high propensity to metastasize. Despite the importance of metastasis in lung cancer mortality, its underlying cellular and molecular mechanisms remain largely elusive. Here, we identified miRNAs as potent suppressors for lung adenocarcinoma metastasis.

Carvacrol affects breast cancer cells through TRPM7 mediated cell cycle regulation.

As the most prevalent cancer for females, breast cancer is also the second most popular cancer type overall. More efforts are needed to research new drugs and combination therapies for this disease. A naturally derived transient receptor potential melastatin-like 7 channel (TRPM7) inhibitor, carvacrol, was found to have anti-cancer potentials.

Design of UAV Downwash Airflow Field Detection System Based on Strain Effect Principle.

Accurate measurement of the downwash flow field of plant protection unmanned aerial vehicles (UAVs) is essential for analyzing the spatial distribution of droplets. To realize on-line rapid detection of the downwash flow field of a multi-rotor UAV, a flexible polypropylene detection device based on the principle of full bridge strain effect was proposed. Its performance principle was based on the physical deformation caused by wind pressure.

Publisher Correction: Multiplexed enrichment of rare DNA variants via sequence-selective and temperature-robust amplification.

In the version of this Article originally published, owing to a technical error, the Life Sciences Reporting Summary was not included; this summary is now available.

Microdissection of the A01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome.

Background: Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton ( Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding.

Multi-label spacecraft electrical signal classification method based on DBN and random forest.

In spacecraft electrical signal characteristic data, there exists a large amount of data with high-dimensional features, a high computational complexity degree, and a low rate of identification problems, which causes great difficulty in fault diagnosis of spacecraft electronic load systems. This paper proposes a feature extraction method that is based on deep belief networks (DBN) and a classification method that is based on the random forest (RF) algorithm; The proposed algorithm mainly employs a multi-layer neural network to reduce the dimension of the original data, and then, classification is applied. Firstly, we use the method of wavelet denoising, which was used to pre-process the data.

Deficiency of microRNA expands cell fate potential in pluripotent stem cells.

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) efficiently generate all embryonic cell lineages but rarely generate extraembryonic cell types. We found that microRNA deficiency expands the developmental potential of mouse pluripotent stem cells, yielding both embryonic and extraembryonic lineages and strongly inducing MuERV-L (MERVL) endogenous retroviruses, similar to what is seen with features of totipotent two-cell blastomeres. restricts the acquisition of expanded cell fate potential in pluripotent stem cells, and it represses MERVL expression through transcriptional regulation, at least in part by targeting the transcription factor Gata2.

Multiplexed enrichment of rare DNA variants via sequence-selective and temperature-robust amplification.

Rare DNA-sequence variants hold important clinical and biological information, but existing detection techniques are expensive, complex, allele-specific, or don't allow for significant multiplexing. Here, we report a temperature-robust polymerase-chain-reaction method, which we term blocker displacement amplification (BDA), that selectively amplifies all sequence variants, including single-nucleotide variants (SNVs), within a roughly 20-nucleotide window by 1,000-fold over wild-type sequences. This allows for easy detection and quantitation of hundreds of potential variants originally at ≤0.

A Spacecraft Electrical Characteristics Multi-Label Classification Method Based on Off-Line FCM Clustering and On-Line WPSVM.

This paper proposes a novel multi-label classification method for resolving the spacecraft electrical characteristics problems which involve many unlabeled test data processing, high-dimensional features, long computing time and identification of slow rate. Firstly, both the fuzzy c-means (FCM) offline clustering and the principal component feature extraction algorithms are applied for the feature selection process. Secondly, the approximate weighted proximal support vector machine (WPSVM) online classification algorithms is used to reduce the feature dimension and further improve the rate of recognition for electrical characteristics spacecraft.

Postoperative prophylaxis of venous thromboembolism (VTE) in patients undergoing high ligation and stripping of the great saphenous vein (GSV).

To analyze the necessity of venous thromboembolism (VTE) prophylaxis for patients undergoing high ligation and stripping of the great saphenous vein (GSV) and to estimate the efficacy and safety of different anticoagulant protocols in a single-center randomized controlled trial with large sample size. A total of 2196 patients undergoing high ligation and stripping of the GSV were randomized to one of the following postoperative VTE prophylaxis protocols: group A, no VTE prophylaxis (n=542); group B, subcutaneous low-dose unfractionated heparin (LDUH) hypodermic injection, 125 U/kg per day in three divided doses (n=531); group C, low-molecular-weight heparin (LMWH) 6000 IU once a day (n=573); and group D, LMWH 4000 IU twice daily (n=550). Groups were compared for the incidence of VTE and major hemorrhage within 1 month following surgery.

TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells.

It is widely accepted that somatic cells can be reprogrammed by a set of transcription factors to become embryonic stem cell-like: These reprogrammed cells, induced pluripotent stem cells (iPSCs), are nearly identical to embryonic stem cells (ESCs), because both have the capacity to self-renew and to form all cellular lineages of the body. Transcriptional differences between ESCs, iPSCs, and fibroblasts can be analyzed by quantitative PCR (qPCR) using TaqMan(®) Gene Expression assays, a widely used tool for rapid analysis of different cell types. In this chapter, we describe the OpenArray(®) platform which generates qPCR data from high-throughput instrumentation.

Multiple roles for CCR2 during fracture healing.

Bone injury induces an inflammatory response that involves neutrophils, macrophages and other inflammatory cells. The recruitment of inflammatory cells to sites of injury occurs in response to specific signaling pathways. The CC chemokine receptor type 2 (CCR2) is crucial for recruiting macrophages, as well as regulating osteoclast function.

Osteoclasts - the innate immune cells of the bone.

Osteopenia and periarticular bony erosion are consequences of chronic inflammatory autoimmune disease due to an imbalance of osteoclast activity relative to new bone formation. Osteoclasts, which are specialized as the only bone resorbing cell type, are differentiated from hematopoietic myeloid precursor cells. Inflammatory signals mediated by multiple types of immune cells and cytokines have significant influence over osteoclast differentiation and function through direct effects on osteoclast precursors and indirect effects via osteoblasts and other cells in the bony microenvironment including synovial cells, stromal cells, osteocytes and chondrocytes.

Bone microenvironment specific roles of ITAM adapter signaling during bone remodeling induced by acute estrogen-deficiency.

Immunoreceptor tyrosine-based activation motif (ITAM) signaling mediated by DAP12 or Fcepsilon receptor Igamma chain (FcRgamma) have been shown to be critical for osteoclast differentiation and maturation under normal physiological conditions. Their function in pathological conditions is unknown. We studied the role of ITAM signaling during rapid bone remodeling induced by acute estrogen-deficiency in wild-type (WT), DAP12-deficient (DAP12-/-), FcRgamma-deficient (FcRgamma-/-) and double-deficient (DAP12-/-FcRgamma-/-) mice.

Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies.

Defective receptor editing or defective B cell checkpoints have been associated with increased frequency of multireactive autoantibodies in autoimmune disease. However, Ig somatic hypermutation and/or class switch recombination may be mechanisms enabling the development of pathogenic multireactive autoantibodies. In this study, we report that, in the BXD2 mouse model of autoimmune disease, elevated expression of activation-induced cytidine deaminase (AID) in recirculating follicular CD86(+) subsets of B cells and increased germinal center B cell activity are associated with the production of pathogenic multireactive autoantibodies.

Tumor necrosis factor ligand-receptor superfamily and arthritis.

The current studies of apoptosis in rheumatoid arthritis (RA) suggest that the TNF ligand-receptor superfamily (TNFRsF) molecules, downstream pathways (activation of proapoptosis or anti-apoptosis pathway), cell types (lymphocytes and synovial fibroblast), and the mechanism that triggers apoptosis (tolerance induction-related, downmodulation of inflammation-related, or DNA damage-related) all exhibit a capability to determine the induction or prevention of RA. This series of defects at different levels and in different cells have been shown to lead to T cell and synovial hyperproliferation, defective apoptosis, excessive apoptosis, or bone erosion. In this chapter, we summarize the available knowledge of the regulation of TNFRsF and their likely pathogenic roles in RA to help identify candidate target cells and target molecules for delivery of gene constructs to modulate apoptosis to prevent the development of RA in both humans and mice.

Synovial fibroblasts promote osteoclast formation by RANKL in a novel model of spontaneous erosive arthritis.

Objective: Erosion of cartilage and bone is a hallmark of rheumatoid arthritis (RA). This study was undertaken to explore the roles of hyperproliferating synovial fibroblasts and macrophages in abnormal osteoclast formation, using the recently described BXD2 mouse model of RA.

Methods: Cell distribution in the joints was analyzed by immunohistochemistry, using tartrate-resistant acid phosphatase (TRAP) staining to identify osteoclasts.

Molecules mimicking Smad1 interacting with Hox stimulate bone formation.

Bone morphogenetic proteins (BMPs) induce osteoblast differentiation and bone formation. Smads, a group of functionally and structurally related intracellular effectors, mediate signaling initiated by BMPs and regulate cell definite commitment. Previously, we showed that Smad1 activates osteopontin and osteoprotegerin gene expression by dislodging Hoxc-8 from its DNA binding sites.

Smad4 as a transcription corepressor for estrogen receptor alpha.

Antiestrogen compounds exhibit a variety of different effects in different tissues and are widely used for the treatment of osteoporosis, breast cancer, and other diseases. Upon examining the molecular mechanisms, we found that Smad4, a common signal transducer in the bone morphogenetic protein (BMP)/transforming growth factor-beta (TGF-beta) signaling pathway, functions as a transcription corepressor for human estrogen receptor alpha (ERalpha). Endogenous ERalpha was co-immunoprecipitated with Smad4, and the interaction was induced by antiestrogen ligands such as tamoxifen, raloxifene, and droloxifen, which was confirmed in chromatin immunoprecipitation assays.

Jab1 antagonizes TGF-beta signaling by inducing Smad4 degradation.

Tumor suppressor Smad4 is the common signaling effector in the transforming growth factor beta (TGF-beta) superfamily. Phosphorylated regulatory Smads (R-Smads) interact with Smad4, and the complex translocates into the nucleus to regulate gene transcription. Proper TGF-beta signaling requires precise control of Smad functions.