IL-15/IL-15Rα-secreting bioengineered adipocytes reactivate NK/CD8 T cells in ovarian and colon cancer ascites.

Malignant ascites (MA) presents a complex clinical challenge, linked inextricably to poor prognosis, chemoresistance, and metastasis of peritoneal carcinomatosis (PC). However, standard therapeutic approaches for managing or preventing MA secondary to PC remain unavailable. Here we display that a bioengineered adipocyte, encapsulating long-chain fatty acids and concurrently secreting IL-15 and IL-15 receptor α (IL-15Rα), markedly extends the half-life and bioactivity of IL-15.

Multiparametric Quantitative Analysis of Photodamage to Skin Using Optical Coherence Tomography.

Ultraviolet (UV) irradiation causes 90% of photodamage to skin and long-term exposure to UV irradiation is the largest threat to skin health. To study the mechanism of UV-induced photodamage and the repair of sunburnt skin, the key problem to solve is how to non-destructively and continuously evaluate UV-induced photodamage to skin. In this study, a method to quantitatively analyze the structural and tissue optical parameters of artificial skin (AS) using optical coherence tomography (OCT) was proposed as a way to non-destructively and continuously evaluate the effect of photodamage.

Microfluidic Particle Separation and Detection System Based on Standing Surface Acoustic Wave and Lensless Imaging.

Objective: Separation and detection of micro-particles or cells from bio-samples by point-of-care (POC) systems are critical for biomedical and healthcare diagnostic applications. Among various microfluidic separation techniques, acoustophoresis-based technique has the advantages of label-free and good biocompatibility. However, most of the existing separation techniques are bulky and require additional equipment for analysis.

Automatic quantitative analysis of structure parameters in the growth cycle of artificial skin using optical coherence tomography.

Significance: Artificial skin (AS) is widely used in dermatology, pharmacology, and toxicology, and has great potential in transplant medicine, burn wound care, and chronic wound treatment. There is a great demand for high-quality AS product and a non-invasive detection method is highly desirable.

Aim: To quantify the constructure parameters (i.

Effect of Tumor Red Blood Cell Immunity and Tumor Cell Cycle in Mice Bearing Solid Liver Cancer with Intelligent Cancer Zhongning Therapeutic Apparatus.

People's unhealthy lifestyles, especially the number of smoking and passive smoking populations, are increasing year by year and the population is aging. With the development and progress of lung cancer cell and molecular biology, the incidence rate and mortality rate of lung cancer are increasing year by year. The existing tumor biochemotherapy has rapidly developed from preliminary research and animal research to clinical research.

Small molecule CDS-3078 induces G/M phase arrest and mitochondria-mediated apoptosis in HeLa cells.

The tumor suppressor p53 serves important roles in cell cycle arrest and apoptosis, and its activation increases the sensitivity of cancer cells to radiotherapy or chemotherapy. In the present study, the small molecule 2-[1-(4-(benzyloxy)phenyl)-3-oxoisoindolin-2-yl)-2-(4-methoxyphenyl)] acetic acid (CDS-3078) significantly increased p53 mRNA expression levels in a dose-dependent manner. Treatment with CDS-3078 increased p53 expression levels and p53-mediated activation of its downstream target genes in HeLa cells.

Histone H2A-peptide-hybrided upconversion mesoporous silica nanoparticles for bortezomib/p53 delivery and apoptosis induction.

The design and development of advanced gene/drug codelivery nanocarrier with good biocompatibility for cancer gene therapy is desirable. Herein, we reported a gene delivery nanoplatform to synergized bortezomib (BTZ) for cancer treatment with histone H2A-hybrided, upconversion luminescence (UCL)-guided mesoporous silica nanoparticles [UCNPs(BTZ)@mSiO-H2A]. The functionalization of H2A on the surface of UCNPs(BTZ)@mSiO nanoparticles realized the improvement of biocompatibility and enhancement of gene encapsulation and transfection efficiency.

CDS-1548 induces apoptosis in HeLa cells by activating caspase 3.

Cervical cancer continues to be a threat to female health globally. In the present study, the potential anticancer activity of 2-[2-hydroxyl-1-(4-methoxy phenyl) ethyl]-3-(4-benzyloxy phenyl) isoindolin-1-one (CDS-1548), was evaluated in HeLa cells. CDS-1548 is an organic small-molecule compound characterized by two chiral centers, with the nuclear parent 1H-isoindolin-1-one.

Digital holographic phase imaging with aberrations totally compensated.

Digital holography is a well-accepted method for phase imaging. However, the phase of the object is always embedded in aberrations. Here, we present a digital holographic phase imaging with the aberrations fully compensated, including the high order aberrations.

Synergistic apoptotic effects of silibinin in enhancing paclitaxel toxicity in human gastric cancer cell lines.

Gastric cancer (GC) is the 3rd leading cause of tumor‑associated mortality worldwide. The efficacy of paclitaxel, a frequently used GC chemotherapeutic agent, is hindered due to drug resistance, dose‑induced toxicity and adverse side effects. Silibinin, an active compound of a widely consumed dietary supplement, milk thistle extract, has recently been demonstrated to have strong antitumor efficacy in a human GC cell model.

A Surface Acoustic Wave Pumped Lensless Microfluidic Imaging System for Flowing Cell Detection and Counting.

The future point-of-care diagnostics requires miniaturizing the existing bulky and expensive bioanalysis instruments, where lab-on-CMOS-chip-based technology can provide a promising solution. In this paper, we presented a surface acoustic wave (SAW) pumped lensless microfluidic imaging system for flowing cell detection and counting. Different from the previous lensless systems, which employ external bulky syringe pump for cell driven, the developed system directly integrates the SAW pump on the CMOS image sensor chip to drive the cell-containing microfluid.

Cytotoxicity of CdTe quantum dots in human umbilical vein endothelial cells: the involvement of cellular uptake and induction of pro-apoptotic endoplasmic reticulum stress.

Cadmium telluride quantum dots (CdTe QDs) have been proposed to induce oxidative stress, which plays a crucial role in CdTe QDs-mediated mitochondrial-dependent apoptosis in human umbilical vein endothelial cells (HUVECs). However, the direct interactions of CdTe QDs with HUVECs and their potential impairment of other organelles like endoplasmic reticulum (ER) in HUVECs are poorly understood. In this study, we reported that the negatively charged CdTe QDs (-21.

DNA-Hybrid-Gated Photothermal Mesoporous Silica Nanoparticles for NIR-Responsive and Aptamer-Targeted Drug Delivery.

Near-infrared light is an attractive stimulus due to its noninvasive and deep tissue penetration. Particularly, NIR light is utilized for cancer thermotherapy and on-demand release of drugs by the disruption of the delivery carriers. Here we have prepared a novel NIR-responsive DNA-hybrid-gated nanocarrier based on mesoporous silica-coated Cu1.

Nano-p-n junction heterostructure TiO nanobelts for the electrochemical detection of anticancer drug and biointeractions with cancer cells.

Nano-p-n junction heterostructures based on TiO nanobelts with enhanced (001) facets were produced by assembling p-type semiconductor NiO nanoparticles on n-type surface-coarsened TiO nanobelt surfaces. The heterostructures were then used as the sensing electrode for the electrochemical detection of anticancer drugs O-benzylguanine (OBG) and lung cancer cells. OBG exhibited an irreversible diffusion-controlled electrochemical process with an oxidation peak clearly identified at +0.

[New compound NL-608 (a nutlin analog) induces apoptosis in human breast cancer MCF-7 cells].

Objective: To observe the effect of NL-608 (a nutlin analog) on apoptosis induction in human breast cancer MCF-7 cells in vitro, and investigate the relevant molecular mechanism.

Methods: The effect of NL-608 on proliferation of MCF-7 cells was determined by MTT assay. The apoptosis in MCF-7 cells was determined by flow cytometry with annexin V-FITC and PI.

Silibinin triggers apoptosis and cell-cycle arrest of SGC7901 cells.

Silibinin, a flavonoid compound, has shown to be of chemopreventive potential against many cancers. However, its efficacy against gastric cancer has not been well elucidated. Here, we assessed the activity of Silibinin on apoptosis and cell-cycle arrest in human gastric cells culture system using SGC-7901 as the model.

Silibinin causes apoptosis and cell cycle arrest in some human pancreatic cancer cells.

Silibinin, an effective anti-cancer and chemopreventive agent in various epithelial cancer models, has been reported to inhibit cancer cell growth through mitogenic signaling pathways. However, whether it can inhibit human pancreatic carcinoma growth and what are the underlying mechanisms is still not well elucidated. Here, we evaluated the inhibitory proliferation effects of Silibinin in pancreatic carcinoma growth and examined whether Silibinin modulates cell cycle and apoptosis.

Changes of constituents and activity to apoptosis and cell cycle during fermentation of tea.

Tea is believed to be beneficial for health, and the effects of the fermentation process on its contributions to apoptosis and cell cycle arrest of gastric cancer cells have not been completely investigated. In this study, the chemical components in green tea, black tea and pu-erh tea aqueous extracts were analyzed and compared. The polysaccharide and caffeine levels were substantially higher in the fermented black tea and pu-erh tea, while the polyphenol level was higher in the unfermented green tea.

Fabricating a pearl/PLGA composite scaffold by the low-temperature deposition manufacturing technique for bone tissue engineering.

Here we developed a composite scaffold of pearl/poly(lactic-co-glycolic acid) (pearl/PLGA) utilizing the low-temperature deposition manufacturing (LDM). LDM makes it possible to fabricate scaffolds with designed microstructure and macrostructure, while keeping the bioactivity of biomaterials by working at a low temperature. Process optimization was carried out to fabricate a mixture of pearl powder, PLGA and 1,4-dioxane with the designed hierarchical structures, and freeze-dried at a temperature of -40 degrees C.

An cell-assembly derived physiological 3D model of the metabolic syndrome, based on adipose-derived stromal cells and a gelatin/alginate/fibrinogen matrix.

One of the major obstacles in drug discovery is the lack of in vitro three-dimensional (3D) models that can capture more complex features of a disease.Here we established a in vitro physiological model of the metabolic syndrome (MS) using cell-assembly technique (CAT), which can assemble cells into designated places to form complex 3D structures. Adipose-derived stromal (ADS) cells were assembled with gelatin/alginate/fibrinogen.

Dynamic monitoring of changes in endothelial cell-substrate adhesiveness during leukocyte adhesion by microelectrical impedance assay.

Adhesion of leukocytes to endothelial cells in inflammation processes leads to changes of endothelial cell-substrate adhesiveness, and understanding of such changes will provide us with important information of inflammation processes. In this study, we used a noninvasive biosensor system referred to as real-time cell electronic sensor (RT-CES) system to monitor the changes in endothelial cell-substrate adhesiveness induced by human monoblastic cell line U937 cell adhesion in a dynamic and quantitative manner. This assay, which is based on cell-substrate impedance readout, is able to monitor transient changes in cell-substrate adhesiveness as a result of U937 cell adhesion.

Astragaloside IV inhibits proliferation and promotes apoptosis in rat vascular smooth muscle cells under high glucose concentration in vitro.

Astragaloside IV is one of the main active ingredients of Radix astragali, which is a herbal remedy widely used in traditional Chinese medicine for the treatment of diabetes and cardiovascular diseases. However, its effects on vascular smooth muscle cell (VSMC), which plays a key role in the development of diabetic vascular complications, were not well studied. The present study was performed to examine the effects of astragaloside IV on proliferation, apoptosis and phenotypic modulation of VSMC under high D-glucose (25 mM).

Redistribution of intracellular calcium and its effect on apoptosis in macrophages: Induction by oxidized LDL.

Calcium signaling, as a key to early step of the elementary intracellular events, has been implicated in controlling the development of atherosclerosis. We have shown previously that oxidized low density lipoprotein OxLDL-induced spatiotemporal increases of intracellular free calcium ([Ca(2+)](i)) in the early formation of macrophage foam cells. Here, we evaluated how spatiotemporal redistribution of intracellular calcium occurs and would affect OxLDL-induced apoptosis.

Salidroside stimulated glucose uptake in skeletal muscle cells by activating AMP-activated protein kinase.

In the present study, we reported the metabolic effects of salidroside, one of the active components of Rhodiola Rosea, on skeletal muscle cells. Salidroside dose-dependently stimulated glucose uptake in differentiated L6 rat myoblast cells. Inhibitor of AMP-activated protein kinase (AMPK) by pretreating the cells with compound C potently reduced salidroside-stimulated glucose uptake, while inhibition of phosphatidylinositol 3-kinase (PI3K) by wortmannin exhibited no significant inhibitory effect on salidroside-mediated glucose transport activation.

B-type natriuretic peptide enhances mild hypoxia-induced apoptotic cell death in cardiomyocytes.

In the case of left ventricle remodeling after myocardial infarction, cardiomyocyte apoptosis is attributed to increased cardiac workload by the stimulus such as chronic hypoxia. B-Type natriuretic peptide, being known as a reliable prognostic of cardiovascular pathology, plays an important role in the myocardial infarction. However, the action of B-type natriuretic peptide on cardiomyocytes undergoing apoptosis is unclear.