Identification of Amino Acid Residues Critical for the Interaction of Fibrin with N-Cadherin.

We recently identified N-cadherin as a novel receptor for fibrin and localized complementary binding sites within the fibrin βN-domains and the third and fifth extracellular domains (EC3 and EC5) of N-cadherin. We also hypothesized that the His16 and Arg17 residues of the βN-domains and the (Asp/Glu)-X-(Asp/Glu) motifs present in the EC3 and EC5 domains may play roles in the interaction between fibrin and N-cadherin. The primary objectives of this study were to test these hypotheses and to further clarify the structural basis for this interaction.

Fabry-Perot Effect Suppression in Gas Cells Used in THz Absorption Spectrometers. Experimental Verification.

A standard measuring gas cell used in absorption spectrometers is a cylinder enclosed by two transparent windows. The Fabry-Perot effects caused by multiple reflections of terahertz waves between these windows produce significant variations in the transmitted radiation intensity. Therefore, the Fabry-Perot effects should be taken into account to correctly measure absorption spectra in Bouguer law-based absorption spectroscopy.

[Antimicrobial activity of nitrofurans (nitrofurantoin, furazidine and furazidine potassium) against uropatogenic Escherichia Coli isolated from patients with lower urinary tract infections].

Currently, the problem of antibiotic resistance is of great relevance to society not only for medical, but also for social and economic reasons. Infections caused by multidrug-resistant pathogens have a longer course, more often require hospitalization, and increase the risk of mortality. The antimicrobial resistance is most relevant for hospitals in the case of the development of nosocomial infections.

Treatment of asymptotic non-adiabatic couplings with higher order reprojection method in the diabatic representation.

The problem of asymptotic non-adiabatic couplings in heavy particle collisions is treated using the reprojection method. The mixing matrix that mixes the asymptotic solutions of the coupled states to obtain appropriate boundary conditions is here derived to second order, yielding a faster convergence of the cross section. In addition, the reprojection method is implemented in a diabatic representation and applied to inelastic scattering of Li + Na and H + H collisions and to mutual neutralization in H+ + H- collisions.

[Pathological tortuosity of intracranial arteries (pure arterial malformation) - diagnosis and management tactics].

Background: Tortuosity of intracranial arteries is rare and usually mistaken for arteriovenous malformations. In the world literature, the term «pure arterial malformations» is used to refer to this disease.

Objective: To summarize the experience of the Burdenko Neurosurgery Center on diagnosis of intracranial artery tortuosity, management and treatment of these patients, as well as to review appropriate literature data.

Complete genome sequence of АМК-16, isolated from a small ruminant in the Middle Volga Region, Russia.

We report the complete genome sequence of the АМК-16, recovered from the aborted caprine fetus during a case of chlamydia infection. This 1,152,497-bp genome with 7,552-bp cryptic plasmid provides novel insights into the genetic diversity of chlamydia agent strains particularly those causing the infection in small ruminants.

Identification of Neural (N)-Cadherin as a Novel Endothelial Cell Receptor for Fibrin and Localization of the Complementary Binding Sites.

Based on the high structural homology between vascular endothelial (VE)-cadherin and neural (N)-cadherin, we hypothesized that fibrin, which is known to interact with VE-cadherin and promote angiogenesis through this interaction, may also interact with N-cadherin. To test this hypothesis, we prepared fibrin and its plasmin-produced and recombinant fragments covering practically all parts of the fibrin molecule. We also prepared the soluble extracellular portion of N-cadherin (sN-cadherin), which includes all five extracellular N-cadherin domains, and studied its interaction with fibrinogen, fibrin, and the aforementioned fibrin fragments using two independent methods, ELISA and SPR.

Surgical treatment of cervical neurovascular bundle tumors.

Objective: To determine the main principles of a patient-oriented individual approach to diagnosis and surgical treatment of cervical neurovascular bundle tumors considering the capabilities of neurosurgical hospital.

Material And Methods: There were 92 patients with cervical soft tissue tumors affecting neurovascular bundle. Age of patients ranged from 9 to 81 years (mean 47).

pConus device for endovascular treatment of basilar bifurcation aneurysms.

Unlabelled: Aneurysm occlusion with microcoils is an effective and safe procedure. However, bifurcation aneurysms usually have a wide neck, and their occlusion is still a difficult objective.

Objective: To evaluate the efficacy and safety of bifurcation stents in the treatment of basilar bifurcation aneurysms.

[Primordial odontogenic tumor in children].

Unlabelled: Primordial odontogenic tumor (POT) is a rare benign mixed epithelial and mesenchymal odontogenic tumor that has been included as a new nosological form in the latest classification of the World Health Organization (WHO 2017). The first two clinical cases of POT treatment in children in Russia are presented. A comprehensive examination and surgical treatment of POT were carried out.

[Rationale for choosing an antibiotic for the treatment of cystitis: recommendations of clinical pharmacologists: A review].

In recent years, the harmonization of domestic and foreign clinical recommendations for the treatment of cystitis has been achieved. Nitrofurans and fosfomycin trometamol are recommended as first line therapy antibiotics, and oral 3rd generation of cephalosporins are recommended as alternative antibiotics; fluoroquinolones are excluded from the recommended medications due to an unfavorable safety profile. The main rationale for inclusion of antibiotics in the recommendations as a first line therapy of cystitis is the level of resistance of uropathogens to antibiotics, primarily Escherichia coli.

Intelligent Decision-Support System for Epidemiological Diagnostics. II. Information Technologies Development, .

The article projects the components of the intelligent decision support system for epidemiological diagnostics and investigates their interaction with the user. The system includes a bank of models and machine learning methods, a bank of population dynamics models, visualization and reporting tools, and management decision-making unit. The concept of information technology to ensure biosafety of the population is provided.

Intelligent Decision-Support System for Epidemiological Diagnostics. I. A Concept of Architecture Design.

The problems of decision support for epidemiological diagnostics are investigated. The basis for supporting decision-making is mathematical tools for analyzing morbidity data, as well as modeling of epidemic processes. The current state of research in this area is analyzed.

Current View on the Molecular Mechanisms Underlying Fibrin(ogen)-Dependent Inflammation.

Numerous studies have revealed the involvement of fibrinogen in the inflammatory response. To explain the molecular mechanisms underlying fibrinogen-dependent inflammation, two bridging mechanisms have been proposed in which fibrin(ogen) bridges leukocytes to endothelial cells. The first mechanism suggests that bridging occurs via the interaction of fibrinogen with the leukocyte receptor Mac-1 and the endothelial receptor ICAM-1 (intercellular adhesion molecule-1), which promotes leukocyte transmigration and enhances inflammation.

Structure and Methylation of 35S rDNA in Allopolyploids (2 = 4 = 32, BBDD) and (2 = 6 = 48, AABBDD) and Their Parental Species Show Evidence of Nucleolar Dominance.

Transcriptional silencing of 35S rDNA loci inherited from one parental species is occurring relatively frequently in allopolyploids. However, molecular mechanisms by which it is selected for transcriptional silencing remain unclear. We applied NGS, silver staining and bisulfite sequencing to study the structure, expression and methylation landscape of 35S rDNA in two allopolyploids of common origin, allotetraploid (2 = 4 = 32, genome composition BBDD) and allohexaploid (2 = 6 = 48, AABBDD), and their genome donors, (2 = 16, AA), (2 = 16, BB) and (2 = 16, DD).

Dual functions of the fibrin βN-domains in the VLDL receptor-dependent pathway of transendothelial migration of leukocytes.

Our previous studies revealed that fibrin interacts with the VLDL receptor (VLDLR) through a pair of its βN-domains and this interaction promotes transendothelial migration of leukocytes and, thereby, inflammation. In agreement, the NDSK-II fragment representing the central part of the fibrin molecule and containing these domains stimulates leukocyte transmigration. However, the recombinant (β15-66) fragment corresponding to a pair of the βN-domains inhibits NDSK-II-stimulated leukocyte transmigration.

On intelligent agent-based simulation of COVID-19 epidemic process in Ukraine.

COVID-19 has impacted all areas of human activity around the world. Modern society has not faced such a challenge. Affordable travel and flights between continents allowed the virus to rapidly spread to all corners of the world.

[Unruptured asymptomatic brain aneurysms: modern approaches to the choice of surgical method and treatment outcomes].

Background: In recent years, significant attention has been paid to preventive vascular neurosurgery. Treatment of unruptured asymptomatic brain aneurysms is one of the sections of this surgery.

Objective: To evaluate treatment outcomes in patients with unruptured asymptomatic brain aneurysms who underwent treatment chosen on the basis of criteria adopted at the Burdenko Neurosurgery Center.

Diversion-p64: results from an international, prospective, multicenter, single-arm post-market study to assess the safety and effectiveness of the p64 flow modulation device.

Background: The use of flow diversion to treat intracranial aneurysms has increased in recent years.

Objective: To assess the safety and angiographic efficacy of the p64 flow modulation device.

Methods: Diversion-p64 is an international, prospective, multicenter, single-arm, study conducted at 26 centers.

[Primordial odontogenic tumor is a new nosological entity in the 2017 WHO classification of Head and Neck Tumors].

The paper describes a case of primordial odontogenic tumor of the mandible, a rare neoplasm that has been recently included into the WHO classification. It presents its clinical, radiological, morphological, and immunohistochemical characteristics.

[Rationale for the choice of an antibiotic for urinary tract infections with an emphasis on the environmental safety of therapy].

Difficulties in prescribing antibiotics for lower urinary tract infections (UTI) are associated with a fact that such patients can be treated not only by urologists, but also by general practitioners, internists, pediatricians, and gynecologists. Therefore, it is important to adapt the practical recommendations for the treatment of cystitis for different medical disciplines. When creating clinical guidelines, experts take into account the different factors in choosing antibiotic therapy.

Structural Basis for the Interaction of Fibrin with the Very Low-Density Lipoprotein Receptor Revealed by NMR and Site-Directed Mutagenesis.

Interaction of fibrin with the very low-density lipoprotein receptor (VLDLR) promotes transendothelial migration of leukocytes and thereby inflammation. To establish the structural basis for this interaction, we have previously localized the VLDLR-binding site to fibrin βN-domains including fibrin β chain sequence 15-64 and determined the NMR solution structure of the VLDLR(2-4) fragment containing fibrin-binding CR domains 2-4 of VLDLR. In this study, we identified amino acid residues in VLDLR and the βN-domains that are involved in the interaction using NMR and site-directed mutagenesis.

[Surgical treatment of vertebral artery confluence aneurysms].

Background: Aneurysms of vertebral artery confluence and proximal parts of basilar artery are extremely rare. They are usually combined with proximal fenestration of basilar artery. No timely surgical treatment of these aneurysms is associated with high risk of adverse outcomes, and their treatment is a challenge for neurosurgeons.