Enzyme-regulated NO programmed to release from hydrogel-forming microneedles with endogenous/photodynamic synergistic antibacterial for diabetic wound healing.

The infection-prone wound pathology microenvironment leads to ulceration and difficult healing of diabetic wounds, which seriously affects the quality of survival of patients. In this study, natural polymer materials gelatin and polylysine were used as substrates. By introducing iron/tannic acid (FeTA) composite nanoparticles with excellent photothermal properties into the system, the glutamine residues of gelatin were crosslinked with the primary ammonia of polylysine by glutamine aminotransferase.

Bio-fabricated nanocomposite hydrogel with ROS scavenging and local oxygenation accelerates diabetic wound healing.

Chronic wounds, especially diabetic wounds, involve abnormally long inflammatory periods due to their pathological microenvironment of high reactive oxygen species (ROS) levels and lack of blood vessels. Here, a mild, simple and feasible fabrication approach, a sustained oxygenation system is proposed, consisting of MnO nanosheets and a dual-network hydrogel fabricated from natural biomaterials including silk fibroin (SF) and carboxymethyl cellulose (CMC). Compared with the initial value (61.

Ascidian-inspired aciduric hydrogels with high stretchability and adhesiveness promote gastric hemostasis and wound healing.

Adhesives for gastric hemorrhage are of great clinical significance. However, it remains a major challenge in clinics due to its poor stability under acidic environments and low adhesion to wet tissues. Herein, inspired by the high adhesiveness of the ascidian secretory protein, we designed a series of aciduric bionic hydrogel adhesives (PDTAs) based on poly(γ-glutamic acid) (γ-PGA) and tannic acid (TA).

Dynamic regulable sodium alginate/poly(γ-glutamic acid) hybrid hydrogels promoted chondrogenic differentiation of stem cells.

Traditional hydrogels often fail to match the dynamic interactions between mechanical and cellular behaviors exhibited by the natural cartilage extracellular matrix. In this research, we constructed a novel hybrid hydrogels system based on sodium alginate and polyglutamic acid. By controlling the grafting rate and concentration of polymer, the gelation time and mechanical strength can be adjusted between range of 8-28 s and 60-144 kPa.

Bio-inspired hydrogel-based bandage with robust adhesive and antibacterial abilities for skin closure.

Unlabelled: Although conventional suturing techniques are commonly used in assisting wound closure, they do pose limited conduciveness and may lead to secondary injury to wound tissues. Inspired by marine organism mussels, we designed and manufactured a bio-inspired hydrogel-based bandage with tough wet tissue adhesion to substitute traditional surgical suture, accelerate wound healing and avoid infection. Poly(γ-glutamic acid) was modified with 3,4-dihydroxyphenylalanine and glycidyl methacylate, then introduced into the acrylic acid--acrylamide hydrogel matrix with robust mechanical properties.

3D-printed antioxidant antibacterial carboxymethyl cellulose/ε-polylysine hydrogel promoted skin wound repair.

Developing a wound dressing for the treatment of large and irregular-shaped wounds remains a great challenge. Herein we developed novel printable bionic hydrogels with antibacterial and antioxidant properties which could effectively overcome the challenge by inhibiting inflammation and accelerating wound healing. The CMC/PL (CP) hydrogels were customized with glycidyl methacrylate (GMA) modified carboxymethyl cellulose (CMC) and ε-polylysine (ε-PL) via ultraviolet (UV) light polymerization using a 3D printer.

Cloning, Yeast Expression, and Characterization of a β-Amyrin C-28 Oxidase (CYP716A249) Involved in Triterpenoid Biosynthesis in Polygala tenuifolia.

Polygala tenuifolia Willd. is a traditional Chinese herbal medicine that is widely used in treating nervous system disorders. Triterpene saponins in P.

Mussel-Inspired Dual-Cross-linking Hyaluronic Acid/ε-Polylysine Hydrogel with Self-Healing and Antibacterial Properties for Wound Healing.

Physicians have long been calling for an inherent antimicrobial wound dressing, which will be a great progress for treating complicated infections. Here, we report a novel bioadhesive hydrogel with inherent antibacterial properties prepared by mixing modified hyaluronic acid (HA) and ε-polylysine (EPL). This hydrogel can effectively kill Gram (+) and (-) bacteria for its high positive charge density on the surface.

Boron-assisted dual-crosslinked poly (γ-glutamic acid) hydrogels with high toughness for cartilage regeneration.

Although mesenchymal stem cells (MSCs) encapsulated with bionic scaffolds have been widely used in treating trauma induced cartilage defects, challenges still persist for these hydrogel scaffolds to create suitable shelters for the MSCs and guide their behaviors. In this work, novel biomimetic hydrogel scaffolds were prepared by thiol-ene Michael addition between glycidyl methacrylate (GMA)-modified poly (γ-glutamic acid) (γ-PGA-GMA) and DL-1,4-Dithiothreitol (DTT) for cartilage tissue engineering. Sodium tetraborate decahydrate was added into the system to connect with DTT through hydrogen-bond interaction and served as catalyst for thiol-ene Michael addition to strengthen the intensity of the hydrogel.

pH-responsive nanomicelles of poly(ethylene glycol)-poly(ε-caprolactone)-poly(L-histidine) for targeted drug delivery.

Here, a novel pH-responsive block copolymer, poly (ethylene glycol)-poly(ε-caprolactone)-poly(L-histidine) (PEG-PCL-PHis), was synthesized and designed for anti-cancer drug delivery with excellent biocompatible, biodegradable, and strong drug loading efficiency. H-NMR, IF-IR, and GPC were used to characterize the structure of the PEG-PCL-PHis copolymer. In addition, the morphology, particle size, Zeta potential, and critical micelle concentration (CMC) of different degree of polymerization were determined by transmission electron microscopy (TEM), dynamic light scattering granulometer (DLS), and fluorescence spectrometer, respectively.

[Analysis of influencing factors of secondary metabolites contents in cultivated Polygala tenuifolia].

This work was launched to explore the effect of habitat and growth year on the secondary metabolites contents of cultivated Polygala tenuifolia. The samples of cultivated P. tenuifolia were analyzed by ultra-high performance liquid chromatography(UPLC)-quadrupole time-of-flight mass spectrometry(Q-TOF MS), and the obtained data were analyzed using multiple statistical analysis and cluster analysis.

Identification of Genes Involved in Flavonoid Biosynthesis in Sophora japonica Through Transcriptome Sequencing.

Sophora japonica is a traditional Chinese medicinal ingredient that is widely used in the medicine, food, and industrial dye industries. Since flavonoids are the main components of S. japonica, studying the flavonoid composition and content of this plant is important.

Utilization of UPLC/Q-TOF-MS-Based Metabolomics and AFLP-Based Marker-Assisted Selection to Facilitate/Assist Conventional Breeding of Polygala tenuifolia.

As one of the most important traditional Chinese medicine, the quality of Polygala tenuifolia is difficult to control and a new method must be established to facilitate/assist the breeding of P. tenuifolia. In this study, UPLC/Q-TOF-MS-based metabolomics analysis was performed to determine the chemical composition and screen metabolite biomarkers according to agronomic traits.