Metaphase Cells Enrichment for Efficient Use in the Dicentric Chromosome Assay.

The dicentric chromosome assay (DCA), is considered the 'gold standard' for radiation biodosimetry. Yet, DCA, as currently implemented, may be impractical for emergency response applications, especially when time is of the essence, owing to its labor-intensive and time-consuming nature. The growth of a primary lymphocyte culture for 48 h in vitro is required for DCA, and manual scoring of dicentric chromosomes (DCs) requires an additional 24-48 h, resulting in an overall processing time of 72-96 h for dose estimation.

Restoration of images captured by a staggered time delay and integration camera in the presence of mechanical vibrations.

Staggered time delay and integration (TDI) scanning image acquisition systems are usually employed in low signal-to-noise situations such as thermal imaging. Analysis and restoration of images acquired by thermal staggered TDI sensors in the presence of mechanical vibrations that may cause space-variant image distortions (severe geometric warps and blur) are studied. The relative motion at each location in the degraded image is identified from the image when a differential technique is used.