Tri-signal CdS@SiO nanoprobes for accurate and sensitive detection of human immunoglobulin G with enhanced flexibility and internal validation.

Accurate and sensitive determination of human immunoglobulin G (HIgG) level is critical for diagnosis and treatment of various diseases, including rheumatoid arthritis, humoral immunodeficiencies, and infectious disease. In this study, versatile tri-signal probes were developed by preparing CdS@SiO nanorods that integrate photoluminescence (PL), multi-phonon resonant Raman scattering (MRRS) and infrared absorption (IRA) properties. Through the coating of multiple CdS nanoparticles as cores within SiO shells, the PL and MRRS properties of CdS were improved, resulting in a significantly lowered limit of detection (LOD), with the lowest LOD of 12.

Photoluminescent and multi-phonon resonance Raman scattering dual-mode immunoassays based on CdS nanoparticles for HIgG detection.

A dual-mode immunoassay strategy based on CdS nanoparticles as signal probes with both of photoluminescent (PL) and multi-phonon resonance Raman scattering (MRRS) properties was developed. Simplified structural design and preparation were achieved due to the intrinsic integration of PL and MRRS dual signals in the single-unit CdS nanoprobes. Human immunoglobulin G (HIgG) was sensitively and specifically detected using the proposed PL-MRRS dual-mode strategy.

A dual-mode immunosensing strategy for prostate specific antigen detection: Integration of resonance Raman scattering and photoluminescence properties of ZnS:Mn nanoprobes.

Luminescence-based methods are widely used for the detection of prostate specific antigen (PSA), for example during the diagnosis of prostate cancer. However, the accuracy of these methods is sub-optimal. The aim of this study was to develop an accurate and sensitive dual-mode immunosensing technique using a combination of resonance Raman scattering (RRS) and photoluminescence (PL) signals for the detection of PSA.

ALA_PDT Promotes Ferroptosis-Like Death of and Antibiotic Sterilization via Oxidative Stress.

is one of the common clinical non-tuberculous mycobacteria (NTM) that can cause severe skin infection. 5-Aminolevulinic acid photodynamic therapy (ALA_PDT) is an emerging effective antimicrobial treatment. To explore whether ALA_PDT can be used to treat infections, we conducted a series of experiments in vitro.

Identification and Expression Analysis of WRKY Gene Family in Response to Abiotic Stress in .

has become a rare and endangered medicinal plant due to habitat loss in China. As one of the most important and largest transcription factors, WRKY plays a critical role in response to abiotic stresses in plants. However, little is known regarding the functions of the WRKY family in .

A self-floating electrospun nanofiber mat for continuously high-efficiency solar desalination.

Solar desalination is an environment-friendly and sustainable technology to address the shortage of freshwater resources. However, it still faces huge challenges to develop a salt-rejection solar desalination system with continuous high efficiency. Herein, an electrospun nanofiber mat was fabricated for continuously high-efficiency solar desalination with carbon nanotube as a photothermal material, polyvinylidene fluoride as a floating support material and polyvinylpyrrolidone as a pore-forming agent.

Fluorescence-infrared absorption dual-mode nanoprobes based on carbon dots@SiO nanorods for ultrasensitive and reliable detection of carcinoembryonic antigen.

The level of carcinoembryonic antigen (CEA) in serum has the significant reference value for early diagnosis and treatment of various cancers. However, the CEA detection still suffers from the issue of limited sensitivity and reliability. Herein, a fluorescence (FL)-infrared absorption (IRA) dual-mode nanoprobe was fabricated based on carbon dots (CDs)@SiO nanorod for CEA detection.

Upconversion luminescence-infrared absorption nanoprobes for the detection of prostate-specific antigen.

Aiming to the ongoing challenge of accurate and sensitive detection for cancer biomarkers, antibody-functionalized NaYF:Yb, Er@SiO nanorods were developed as upconversion luminescence (UCL)-infrared absorption (IRA) nanoprobes. Benefiting from the shielding effect of the SiO shell, an enhanced UCL was achieved. Additionally, an IRA detection signal was introduced by the Si-O-Si bonds of SiO.

Resonance Raman scattering-infrared absorption dual-mode immunosensing for carcinoembryonic antigen based on ZnO@SiO nanocomposites.

Detection of cancer biomarkers is crucial for the diagnosis and monitoring of malignant tumors. However, the accuracy and sensitivity still require sufficient improvement for practically clinical application. In this work, a reliable and sensitive dual-mode immunosensing method is described for carcinoembryonic antigen (CEA) detection using a biofunctional ZnO@SiO nanocomposite as a resonance Raman scattering (RRS)-infrared (IR) absorption nanoprobe.

Colorimetric determination of copper(II) by using branched-polyethylenimine droplet evaporation on a superhydrophilic-superhydrophobic micropatterned surface.

A colorimetric method is described for the determination of Cu(II). It is based on branched polyethylenimine (BPEI) droplet evaporation on a superhydrophilic-superhydrophobic polystyrene micropatterned surface. Exposure to Cu(II) leads to a color change from colorless to light blue and dark blue.

Recent Advances in Magnetic Upconversion Nanocomposites for Bioapplications.

The combination of magnetism and upconversion luminescent property into one single nanostructure is fascinating for biological fields, such as multimodal bioimaging, targeted drug delivery, and imaging-guided therapy. In this review, we will provide the state-of-the-art advances on magnetic upconversion nanocomposites towards their bioapplications. Their structure design, synthesis methods, surface engineering and applications in bioimaging, drug delivery, therapy as well as biodetection will be covered.

Multifunctional NaYF:Yb,Er@PE@FeO nanocomposites for magnetic-field-assisted upconversion imaging guided photothermal therapy of cancer cells.

Even though various theranostic agents have been exploited for effective cancer therapy over the years, appropriate design and fabrication of theranostic agents with simple composition, convenient preparation, high theranostic efficiency and minimal side effects on non-cancer cells are still urgently needed. Herein, multifunctional NaYF:Yb,Er@polyelectrolyte (PE)@FeO nanocomposites, with upconversion luminescence, superparamagnetism and photothermal performance, are prepared by a layer-by-layer self-assembly technique. Compared with FeO nanoparticles (NPs), the nanocomposites exhibited nearly 2-fold strong absorption at 808 nm, and thus resulted in an enhanced near-infrared photothermal effect.

An infrared IgG immunoassay based on the use of a nanocomposite consisting of silica coated FeO superparticles.

A reliable, rapid and ultrasensitive immunoassay is described for determination of immunoglobulin G (IgG). It is making use of biofunctional magnetite (FeO) superparticles coated with SiO and serving as an infrared (IR) probe. The unique IR fingerprint signals originating from the transverse and longitudinal phonon modes, respectively, of the asymmetric stretching of the Si-O-Si bridges display a satisfactory resistance to optical interference from the environment.

An "off-on" colorimetric and fluorometric assay for Cu(II) based on the use of NaYF:Yb(III),Er(III) upconversion nanoparticles functionalized with branched polyethylenimine.

The authors describe an "off-on" colorimetric and fluorometric assay for the determination of Cu(II). It is based on the use of upconversion nanoparticles (UCNPs) of type NaYF:Yb(III),Er(III) that were functionalized with branched polyethylenimine (BPEI). A color change from colorless to blue occurs within 2 s after addition of Cu(II) to a solution of the modified UCNPs.

Ultra-facile and rapid colorimetric detection of Cu with branched polyethylenimine in 100% aqueous solution.

The detection of copper ion (Cu) has become a long-term and arduous task in the field of environmental monitoring and human health. Current methods based on branched polyethylenimine (BPEI) for the detection of Cu more or less suffer from being time-consuming and having a high cost or complex surface modifications. Herein, an ultra-facile and rapid colorimetric method was explored to detect Cu in 100% aqueous solution by a direct format using only BPEI.

Dual-mode immunoassay based on shape code and infrared absorption fingerprint signals of silica nanorods.

Silica nanorods were synthesized through a simple one-pot emulsion-droplet-based growth method, in which tetraethylorthosilicate (TEOS) was used as the silica source, ammonia as the catalyst, and polyvinylpyrrolidone (PVP) as the structure-directing agent and stabilizer. By controlling hydrolysis and condensation in the reaction process, we regulated the aspect ratios and the infrared (IR) absorption fingerprint signals (the transverse optical and the longitudinal optical phonon modes) of the silica nanorods. Based on this, a dual-mode immunoassay was performed for detecting model target analyte, human IgG.

Solvent-Induced Luminescence Variation of Upconversion Nanoparticles.

Solvent plays a vital role in the syntheses, purifications, and broad applications of upconversion nanoparticles (UCNPs). In this work, the effect of various dispersive solvents, including single solvents and mixed solvents, on the luminescence properties of NaYF:Yb, Er UCNPs was studied systematically. The differences in both upconversion luminescence (UCL) intensities and color outputs of the nanoparticles were observed when dispersing the UCNPs in deuterium oxide, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), ethanol, or water.

Magnetic-bead-based sub-femtomolar immunoassay using resonant Raman scattering signals of ZnS nanoparticles.

Highly sensitive, specific, and selective immunoassays are of great significance for not only clinical diagnostics but also food safety, environmental monitoring, and so on. Enzyme-linked immunosorbent assays and fluorescence-based and electrochemical immunoassays are important intensively investigated immunoassay techniques. However, they might suffer from low sensitivity or false-positive results.

Interplay between Static and Dynamic Energy Transfer in Biofunctional Upconversion Nanoplatforms.

Clarification of the energy-transfer (ET) mechanism is of vital importance for constructing efficient upconversion nanoplatforms for biological/biomedical applications. Yet, most strategies of optimizing these nanoplatforms were casually based on a dynamic ET assumption. In this work, we have modeled quantitatively the shell-thickness-dependent interplay between dynamic and static ET in nanosystems and validated the model in a typical biofunctional upconversion nanoplatform composed of NaYF4:Er, Yb/NaYF4 upconversion nanoparticles (UCNPs), and energy-acceptor photosensitizing molecule Rose Bengal (RB).

Targeted labeling of an early-stage tumor spheroid in a chorioallantoic membrane model with upconversion nanoparticles.

In vivo detection of cancer at an early-stage, i.e. smaller than 2 mm, is a challenge in biomedicine.

Multispectral upconversion luminescence intensity ratios for ascertaining the tissue imaging depth.

Upconversion nanoparticles (UCNPs) have in recent years emerged as excellent contrast agents for in vivo luminescence imaging of deep tissues. But information abstracted from these images is in most cases restricted to 2-dimensions, without the depth information. In this work, a simple method has been developed to accurately ascertain the tissue imaging depth based on the relative luminescence intensity ratio of multispectral NaYF4:Yb(3+),Er(3+) UCNPs.