A Universal Multichannel Platform for Assembling Enzyme-Based Boolean Logic Gates.

Concatenated enzyme-based Boolean logic gates activated with 5 chemical input signals were analyzed with a smartphone photo camera. Simultaneous detection of 32 input combinations was conveniently performed using enzyme-modified fiberglass sensing spots generating fluorescence with different intensities for the 0 and 1 binary outputs. The developed technology offers an easy readout method for multi-channel logic systems.

A universal nanostructured bioanalytical platform for NAD-dependent enzymes based on the fluorescent output reading with a smartphone.

A nanostructured surface composed of SiO nanoparticles (200 nm) deposited on fiberglass was used as a platform for immobilization of NAD-dependent enzymes (alcohol and glucose dehydrogenases). The surface functionalized with the enzymes was further loaded with NAD(P)cofactor and 1-methoxy-5-methylphenazinium methyl sulfate (MPMS) redox species. The designed biocatalytic system was used for fluorescent analysis of substrates (ethanol and glucose used as example analyte substances).