Iron deficiency and toxicity trigger divergent metabolic responses and adaptive plasticity in Ulmus pumila: Insights from integrated transcriptomic and metabolomic analyses.

Iron homeostasis is critical for plant growth; however, the mechanisms underlying responses to iron deficiency and toxicity remain poorly understood. We investigated the adaptive strategies of Ulmus pumila, focusing on leaf physiological, transcriptomic, and metabolomic responses to iron stresses. Both iron deficiency and toxicity impaired chlorophyll biosynthesis, PS II efficiency, and chloroplast ultrastructure, resulting in reduced photosynthetic capacity and etiolation/wilting phenotypes.

Effect of transgene on salt tolerance of tobacco.

To explore the effects of salt-tolerance gene accumulation on salt tolerance in transgenic plant, we used four types of plant expression vector (N27, N28, N29, and N30) carrying mtlD, mtlD + gutD, mtlD + gutD + BADH, mtlD + gutD + BADH + sacB genes respectively, to transform tobacco through Agrobacterium-mediated method. Transgenic lines were identified through polymerase chain reaction (PCR) detection. Transgenic lines and non-transgenic plant (CK) were subjected to 6‰ sodium chloride solution stress; then, fluorescence quantitative PCR (FQ-PCR) and salt tolerance indexes were used to assess characteristics.

The metabolomics analysis of cecal contents elucidates significant metabolites involved in the therapeutic effects of total flavonoids derived from Sonchus arvensis L. in male C57BL/6 mice with ulcerative colitis.

Ulcerative colitis (UC), an inflammatory disease affecting the colon and rectal mucosa, is characterized by chronic and heterogeneous behavior of unknown origin. The primary cause of UC is chronic inflammation, which is closely linked to the development of colorectal cancer. Sonchus arvensis L.

The relationship between atmospheric particulate matter, leaf surface microstructure, and the phyllosphere microbial diversity of Ulmus L.

Background: Plants can retain atmospheric particulate matter (PM) through their unique foliar microstructures, which has a profound impact on the phyllosphere microbial communities. Yet, the underlying mechanisms linking atmospheric particulate matter (PM) retention by foliar microstructures to variations in the phyllosphere microbial communities remain a mystery. In this study, we conducted a field experiment with ten Ulmus lines.

Nanoplastic toxicity induces metabolic shifts in Populus × euramericana cv. '74/76' revealed by multi-omics analysis.

There is increasing global concern regarding the pervasive issue of plastic pollution. We investigated the response of Populus × euramericana cv. '74/76' to nanoplastic toxicity via phenotypic, microanatomical, physiological, transcriptomic, and metabolomic approaches.

Total flavones from ameliorate colitis by adjusting the gut microbiota.

Objective: is traditional Chinese food and medicine. We investigated protective effects of flavones from (SAF) on colitis induced by dextran sulfate sodium (DSS) in mice by regulating gut microbiota (GM).

Method: C57BL/6 mice were divided randomly: control group (CL); DSS group (ML); positive control + DSS group (AN); SAF + DSS (FE) group.

Identification of Differentially Expressed lncRNAs in Response to Blue Light and Expression Pattern Analysis of Hybrid Poplar .

Poplar is an important shelterbelt, timber stand, and city tree species that has been the focus of forestry research. The regulatory role of the long non-coding RNA molecule (lncRNA; length > 200 nt) has been a research hotspot in plants. In this study, seedlings of poplar were irradiated with LED blue and white light, and the Illumina HiSeq 2000 sequencing platform was used to identify lncRNAs.

Analysis of the RNA Editing Sites and Orthologous Gene Function of Transcriptome and Chloroplast Genomes in the Evolution of Five Species.

In this study, the chloroplast genomes and transcriptomes of five genus species were sequenced, characterized, combined, and analyzed. A phylogenetic tree was constructed, including 32 other chloroplast genome sequences of Hydrangeoideae species. The results showed that the five chloroplast genomes were typical circular genomes 156,860-157,025 bp in length, with 37.

Effect of T-DNA Integration on Growth of Transgenic × cv. Neva Underlying Field Stands.

Multigene cotransformation has been widely used in the study of genetic improvement in crops and trees. However, little is known about the unintended effects and causes of multigene cotransformation in poplars. To gain insight into the unintended effects of T-DNA integration during multigene cotransformation in field stands, here, three lines (A1-A3) of × cv.

Conjoint Analysis of Genome-Wide lncRNA and mRNA Expression during the Salicylic Acid Response in .

Long noncoding RNAs (lncRNAs) participate in a wide range of biological processes, but lncRNAs in plants remain largely unknown; in particular, we lack a systematic identification of plant lncRNAs involved in hormone responses. To explore the molecular mechanism of the response of poplar to salicylic acid (SA), the changes in protective enzymes, which are closely related to plant resistance induced by exogenous SA, were studied, and the expression of mRNA and lncRNA were determined by high-throughput RNA sequencing. The results showed that the activities of phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO), in the leaves of , were significantly increased by exogenous SA application.

Virus-like particle-based nanocarriers as an emerging platform for drug delivery.

New nanocarrier technologies are emerging, and they have great potential for improving drug delivery, targeting efficiency and bioavailability. Virus-like particles (VLPs) are natural nanoparticles from animal and plant viruses and bacteriophages. Hence, VLPs present several great advantages, such as morphological uniformity, biocompatibility, reduced toxicity and easy functionalisation.

L. water extract attenuates dextran sulfate sodium-induced colitis by adjusting gut microbiota.

L. (SA) is a traditional Chinese food and medicine termed "Ju Mai Cai". The aim of this study was to investigate the protective effects of an aqueous extract of SA on dextran sulfate sodium (DSS) - induced colitis in mice by adjusting the diversity of gut microbiota.

Cold Resistance of Beihaidao Leaves and Its Chloroplast Genome Structure and Comparison with Celastraceae Species.

Beihaidao is one of the most economically important ornamental species of the genus. There are approximately 97 genera and 1194 species of plants worldwide in this family (Celastraceae). Using Beihaidao, we conducted a preliminary study of the cold resistance of this species, evaluated its performance during winter, assembled and annotated its chloroplast genome, and performed a series of analyses to investigate its gene structure GC content, sequence alignment, and nucleic acid diversity.

Study on biomimetic nano tumor targeted delivery system for chemotherapy-laser immunotherapy.

To explore the immune adjuvant effect of nano mesoporous materials and laser immune effect of cancer therapy combined with chemotherapy, we designed a bionic nano tumor targeting delivery system with homologous cancer cell membrane as the outermost layer. Inorganic mesoporous silica (mSiO) and hydroxyapatite (HAP) are used as the intermediate immune adjuvant layer and drug carrier layer, and gold nanorods(GNR) are used as the core. It degrade to release drug under the condition of low pH value, and the gold nanorods are wrapped for near-infrared laser response.

Application of polygene polymerization for insect-resistant poplar breeding.

A major goal of poplar breeding is to obtain new insect-resistant poplar varieties through genetic engineering. However, engineering poplars with only a single insect-resistant gene has limitations, such as narrow insecticidal bands and the rapid development of insect tolerance to the gene. To expand insecticidal bands, delay the development of insect tolerance, and improve the insect resistance of transgenic poplars, polygene polymerization is increasingly being applied to cultivate insect-resistant poplars.

Serum Metabonomics Reveals Risk Factors in Different Periods of Cerebral Infarction in Humans.

Studies of key metabolite variations and their biological mechanisms in cerebral infarction (CI) have increased our understanding of the pathophysiology of the disease. However, how metabolite variations in different periods of CI influence these biological processes and whether key metabolites from different periods may better predict disease progression are still unknown. We performed a systematic investigation using the metabonomics method.

Niches and Seasonal Changes, Rather Than Transgenic Events, Affect the Microbial Community of × 'Neva'.

Exploring the complex spatiotemporal changes and colonization mechanism of microbial communities will enable microbial communities to be better used to serve agricultural and ecological operations. In addition, evaluating the impact of transgenic plants on endogenous microbial communities is necessary for their commercial application. In this study, microbial communities of × 'Neva' carrying c-Cry3A-BADH genes (ECAA1 line), × 'Neva' carrying genes (ECAB1 line), and non-transgenic × 'Neva' from rhizosphere soil, roots, and phloem collected in different seasons were compared and analyzed.

A Retrospective Survey of the Abortion Outbreak Event Caused by Brucellosis at a Blue Fox Breeding Farm in Heilongjiang Province, China.

Brucellosis is a common zoonosis in China, resulting in abortion in animals. Outbreaks of abortion in blue foxes caused by infection have rarely been reported. In the present study, 3-5 mL blood samples collected from the femoral veins of 10 abortuses of blue foxes were assessed by RBPT (Rose Bengal plate test) and SAT (serum tube agglutination test) to preliminarily investigate the source of infection for the clustering of abortion events at a blue fox farm in Heilongjiang Province.

Exogenous Gene Expression and Insect Resistance in Dual Bt Toxin × 'Neva' Transgenic Plants.

(Bt) insecticidal protein genes are important tools in efforts to develop insect resistance in poplar. In this study, the and Bt toxin genes were simultaneously transformed into the poplar variety × 'Neva' by -mediated transformation to explore the exogenous gene expression and insect resistance, and to examine the effects of Bt toxin on the growth and development of larvae after feeding on the transgenic plant. Integration and expression of the transgenes were determined by molecular analyses and the insect resistance of transgenic lines was evaluated in feeding experiments.

Delivery of RGD-modified liposome as a targeted colorectal carcinoma therapy and its autophagy mechanism.

Liposomes are among the most extensively applied drug carriers due to their excellent biocompatibility, controllable size and ease of modification. In the present study, we prepared untargeted liposomes (LP) and targeting liposomes modified with Arg-Gly-Asp (RGD-LP), and Doxorubicin Hydrochloride (DOX) or fluorescent probe was loaded. RGD-LP/DOX was identified to be uniformly spherical in size 131.

The Relationship between Insect Resistance and Tree Age of Transgenic Triploid Plants.

To explore the stability of insect resistance during the development of transgenic insect-resistant trees, this study investigated how insect resistance changes as transgenic trees age. We selected 19 transgenic insect-resistant triploid lines as plant material. The presence of exogenous genes and Cry1Ac protein expression were verified using polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) analyses.

A d-Peptide Ligand of Integrins for Simultaneously Targeting Angiogenic Blood Vasculature and Glioma Cells.

The current prognosis of glioma patients remains poor after intensive multimodal treatments, which is partially due to the existence of the blood-brain tumor barrier (BBTB). In the present study, a novel "bifunctional ligand" (termed VS) was developed by retro-inverso isomerization. VS is a ligand of integrins highly expressed on glioma cells and tumor neovasculature.

Autophagy induced by bovine viral diarrhea virus infection counteracts apoptosis and innate immune activation.

Bovine viral diarrhea virus (BVDV) is an important pathogen of cattle that plays a complex role in disease. There are two biotypes of BVDV: non-cytopathic (NCP) and cytopathic (CP). One strategy that has been used to treat or prevent virus-associated diseases is the modulation of autophagy, which is used by the innate immune system to defend against viral infection; however, at present, the interplay between autophagy and BVDV remains unclear.

The first complete chloroplast genome sequences of Ulmus species by de novo sequencing: Genome comparative and taxonomic position analysis.

Elm (Ulmus) has a long history of use as a high-quality heavy hardwood famous for its resistance to drought, cold, and salt. It grows in temperate, warm temperate, and subtropical regions. This is the first report of Ulmaceae chloroplast genomes by de novo sequencing.