Publications by authors named "Ya-ya Pian"
Article Synopsis
- The study investigates systemic RNA oxidative damage during infections caused by a specific pathogen, focusing on the changes in blood cells and inflammatory responses in a rat model of acute intestinal infection.
- Findings reveal that the infection leads to significant increases in various immune markers and cytokines, and notably, urinary levels of 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), which indicate RNA oxidation.
- The results suggest that urinary 8-oxo-Gsn could serve as a promising biomarker for determining the severity and outlook of infections caused by this pathogen, as levels return to normal upon recovery.
More and more evidence support the concept that RNA oxidation plays a substantial role in the progress of multiple diseases; however, only a few studies have reported RNA oxidation caused by microbial pathogens. Urinary 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGsn), which are broadly used as indicators of oxidative damage of RNA and DNA, were analyzed in this study to determine which can be used as a biomarker of infection in challenged with (). In this work, 24 specific-pathogen-free (SPF) male SD rats were randomly divided into two groups: an infection group and a phosphate-buffered saline (PBS) control group.
View Article and Find Full Text PDFEmerging evidence suggests that microbial pathogens may induce oxidative stress in infected hosts. The aim of the present study was to investigate the relationship between changes in oxidative stress and intestinal infection with and without antibiotic treatment in animal models. Sprague-Dawley (SD) rats were divided into three groups: rats infected with Salmonella enterica serovar Enteritidis (S.
View Article and Find Full Text PDF[Expression, purification and protective antigen analysis of cell wall protein MRP of Streptococcus suis type 2].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
February 2012
Aim: To amplify the mrp gene of Streptococcus suis type 2 05ZYH33, express it in E.coli BL21 in order to acquire high purity recombinant protein MRP, then evaluate the protective antigen of recombinant protein MRP.
Methods: Using PCR technology to obtain the product of mrp gene of 05ZYH33, and then cloned it into the expression vector pET28a(+).