Ruxolitinib induces apoptosis and pyroptosis of anaplastic thyroid cancer via the transcriptional inhibition of DRP1-mediated mitochondrial fission.

Anaplastic thyroid carcinoma (ATC) has a 100% disease-specific mortality rate. The JAK1/2-STAT3 pathway presents a promising target for treating hematologic and solid tumors. However, it is unknown whether the JAK1/2-STAT3 pathway is activated in ATC, and the anti-cancer effects and the mechanism of action of its inhibitor, ruxolitinib (Ruxo, a clinical JAK1/2 inhibitor), remain elusive.

Anterior segment OCT application in quantifying posterior capsule opacification severity with varied intraocular lens designs.

Aim: To evaluate the application of anterior segment-optical coherence tomography (AS-OCT) in posterior capsule opacification (PCO) severity assessment and analyse the relationship between PCO severity and intraocular lens (IOL) characters.

Methods: PCO patients were prospectively recruited. Cross-sectional images of the anterior segment at horizontal and vertical meridians were acquired with AS-OCT.

[Characteristics and Significance of Stable Isotopes and Hydrochemistry in Surface Water and Groundwater in Nanxiaohegou Basin].

The Nanxiaohegou basin is a typical watershed in the gully region of the Loess Plateau, China. The stable isotopes of hydrogen and oxygen and the hydrochemistry of the surface water and groundwater in the region were characterized in this study. The interaction between the surface water and groundwater in the region was demonstrated based on stable isotopes and hydrochemical methods.

CD8 cytotoxic and FoxP3 regulatory T lymphocytes serve as prognostic factors in breast cancer.

Background: There is conflicting evidence regarding the prognostic value of cytotoxic T cell infiltration in breast cancer. The aims of this study were to detect the expression levels and localization of FoxP3 and CD8 in invasive ductal carcinoma of the breast and to investigate the correlations among FoxP3 regulatory T cells (Tregs), CD8 cytotoxic T lymphocytes (CTLs), clinicopathological features, and prognosis in patients with breast cancer.

Methods: Immunohistochemistry was used to detect the expression levels and localization of FoxP3 and CD8.

Quantitative analysis of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in eggs via liquid chromatography-electrospray ionization tandem mass spectrometry.

A widely applicable method involving liquid chromatography-electrospray ionization tandem triple quadrupole mass spectrometry (LC-ESI/MS/MS) was developed for the simultaneous determination of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in eggs. Samples were extracted with ethyl acetate-acetonitrile-ammonium hydroxide (49:49:2, v/v) and defatted with hexane saturated with acetonitrile. The analysis was carried out on a mass spectrometer via an electrospray interface operated in the positive and negative ionization modes using deuterated chloramphenicol-d5 as the internal standard.

[From random mutagenesis to precise genome editing: the development and evolution of genome editing techniques in Drosophila].

Drosophila melanogaster, an important model organism for studying life science, has contributed more to the research of genetics, developmental biology and biomedicine with the development of genome editing techniques. Drosophila genome-editing techniques have evolved from random mutagenesis to precise genome editing and from simple mutant construction to diverse genome editing methods since the 20th century. Chemical mutagenesis, using Ethyl methanesulfonate (EMS), is an important technique to study gene function in forward genetics, however, the precise knockout of Drosophila genes could not be achieved.

Comparison of the Retinal Straylight in Pseudophakic Eyes with PMMA, Hydrophobic Acrylic, and Hydrophilic Acrylic Spherical Intraocular Lens.

Purpose. To investigate the intraocular straylight value after cataract surgery. Methods.

Effects of Notch-1 down-regulation on malignant behaviors of breast cancer stem cells.

This study examined the effect of Notch-1 signaling on malignant behaviors of breast cancer cells by regulating breast cancer stem cells (BCSCs). BCSCs were enriched by using serum-free medium and knocked out of Notch-1 by using a lentiviral vector. Real-time polymerase chain reaction (RT-PCR) and Western blotting were used to detect the Notch-1 expression levels in breast cancer cell lines and BCSCs, and flow cytometry to detect the proportion of BCSCs in BCSC spheres.

[The value of Pentacam in diagnosis of intralenticular foreign body].

Objective: To study the value of Pentacam in the diagnosis and localization of intralenticular foreign body.

Methods: It was a comparative study. In this retrospective study, 9 cases with intralenticular foreign bodies were examined by using slit-lamp, B-mode ultrasound, ultrasound biomicroscopy (UBM), CT and Pentacam.

[Distribution character of synthetic musks in urban sewage sludges].

The characteristics of occurrence and distribution of 8 synthetic musks in 15 sewage sludges in Shanghai were studied. The results indicated that HHCB and AHTN were the two main components in all sludges, with the mean concentration of 2.92 mg x kg(-1) (0.