[Comparative study of iRoot SP and AH Plus on root canal sealing ability in periodontally compromised teeth].

Purpose: This study was aimed to compare the difference between iRoot SP and AH Plus on root canal sealing ability for teeth extracted due to severe periodontitis and explore whether the dentin tubule pathway plays an important role in the development of endodontic-periodontic lesions(EPL), in order to provide a theoretical basis for selection of proper time for root canal therapy and suitable root canal sealants in patients with EPL.

Methods: Fifty single-root anterior teeth extracted due to severe periodontitis were selected. The roots were completely debrided to remove the calculus, dental plaque and cementum.

The role of ALDHs in lipid peroxidation-related diseases.

Lipid peroxidation presents the oxidative degradation of polyunsaturated fatty acids lincited by reactive species. Excessive accumulation of lipid peroxidation byproducts, including 4-hydroxy-2-nonenal (4-HNE) and malondialdehyde (MDA), causes protein dysfunction and various illnesses. Aldehyde dehydrogenases (ALDHs) catalyze the metabolism of both endogenous and exogenous aldehydes.

The Effects of Ligands with Different Electron Donation on the Nitrogen Reduction Performance of Constructed Co-MOFs: NH or NH?

Four cobalt-based metal-organic frameworks (Co-MOFs) based on different ligands with varying electron-donating properties (dpt = 2,5-di(pyridin-4-yl)-1,3,4-thiadiazole, NH₂bdc = 5-aminoisophthalic acid, OHbdc = 5-hydroxyisophthalic acid, H₂tdc = thiophene-2,5-dicarboxylic acid, and hfipbb = 4,4'-(hexafluoropropane-2,2-diyl)bisbenzoic acid) are synthesized. Among them, Co-dpt-NH₂bdc demonstrates the highest nitrogen reduction reaction activity, achieving an ammonia (NH) yield of 68.61 µg·h⁻¹·mg⁻¹ and Faraday efficiency (FE) of 6.

[ Amplification of NK Cells from Feeder Layer Cells Expressing IL-21].

Objective: To investigate the effect of feeder layer cells expressing interleukin (IL)-21 on the amplification of NK cells .

Methods: The K562 cell line with IL-21 expression on its membrane was constructed by electroporation, and co-cultured with NK cells after inactivation. The proliferation of NK cells was observed.

Jianpi-Huatan-Huoxue-Anshen formula ameliorates gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with H22 hepatocellular carcinoma.

Background: Jianpi-Huatan-Huoxue-Anshen formula [Tzu-Chi cancer-antagonizing & life-protecting II decoction (TCCL)] is a Chinese medical formula that has been clinically shown to reduce the gastrointestinal side effects of chemotherapy in cancer patients and improve their quality of life. However, its effect and mechanism on the intestinal microecology after chemotherapy are not yet clear.

Aim: To discover the potential mechanisms of TCCL on gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with hepatocellular carcinoma (HCC).

Translation of Mutant Repetitive Genomic Sequences in and Changes in the Secondary Structures and Functional Specifications of the Encoded Proteins.

Multiple repetitive sequences of authentic genes commonly exist in fungal genomes. AT-biased genotypes of have been hypothesized as repetitive pseudogenes in the genome of (GC-biased Genotype #1 of ) and are generated through repeat-induced point mutation (RIP), which is charactered by cytosine-to-thymine and guanine-to-adenine transitions, concurrent epigenetic methylation, and dysfunctionality. This multilocus study examined repetitive sequences in the genome and transcriptome using a bioinformatic approach and revealed that 8.

Dog10K: an integrated Dog10K database summarizing canine multi-omics.

The diversity observed in canine breed phenotypes, together with their risk for heritabily disorders of relevance to dogs and humans, makes the species an ideal subject for studies aimed at understanding the genetic basis of complex traits and human biomedical models. Dog10K is an ongoing international collaboration that aims to uncover the genetic basis of phenotypic diversity, disease, behavior, and domestication history of dogs. To best present and make the extensive data accessible and user friendly, we have established the Dog10K (http://dog10k.

Research advance in effects of solar radiation on litter decomposition in terrestrial ecosystems.

Litter decomposition significantly influences the carbon (C) dynamics of terrestrial ecosystems. Solar radiation is not only essential for photosynthetic C fixation and primary productivity, but also can directly or indirectly promote litter decomposition through photodegradation. Recently, photodegradation has been identified as a key factor driving litter decomposition and potentially impacts terrestrial C cycle.

Insights into bioaerosol contamination in the process of mineralized refuse mining: Microbial aerosolization behavior and potential pathogenicity.

The landfill mining process is a main source of anthropogenic bioaerosol release, posing potential risks to the health of occupationally exposed personnel and nearby residents. In this study, microbial aerosolization behavior and potential pathogenicity during the landfill mining process were systematically investigated. The highest concentration of bacterial aerosols was measured in the refuse mining area, with a value of 5968 ± 1608 CFU/m, while the highest concentration of fungal aerosols was 1196 ± 370 CFU/m in the refuse screening area.

[Analysis of ACADVL gene variant in a Chinese pedigree affected with Very-long-chain acl-CoA dehydrogenase deficiency].

Objective: To carry out genetic testing on a child diagnosed with Very-long-chain acyl-CoA dehydrogenase deficiency (VLADD) in order to provide a basis for genetic counseling and prenatal diagnosis for his family.

Methods: Whole exome sequencing was performed for the proband. Candidate variant sites in the ACADVL gene were verified by Sanger sequencing, and their pathogenicity was predicted based on the guidelines from the American College of Medical Genetics and Genomics (ACMG).

[Spatiotemporal Characteristics and Influencing Factors of the Synergistic Effect of Pollution Reduction and Carbon Reduction in China].

Based on the use of the coupling coordination model to calculate the coupling coordination degree of carbon emission and pollutant control, the national, regional, and provincial spatiotemporal characteristics of the synergistic effect of pollution control and carbon emissions reduction in China were further analyzed, facilitating the crucial to identification of key areas. The fixed effects regression models and provincial panel data from 2006 to 2020 were used to explore factors contributing to better synergizing the reduction of pollution and carbon emissions in China. On this basis, the adjustment variable of R&D investment intensity was introduced, and the regulation effect model was constructed to further explore the influence mechanism of the synergistic effect of pollution reduction and carbon reduction.

PDIA3 defines a novel subset of adipose macrophages to exacerbate the development of obesity and metabolic disorders.

Adipose tissue macrophages (ATMs) play important roles in maintaining adipose tissue homeostasis and orchestrating metabolic inflammation. Given the extensive functional heterogeneity and phenotypic plasticity of ATMs, identification of the authentically pathogenic ATM subpopulation under obese setting is thus necessitated. Herein, we performed single-nucleus RNA sequencing (snRNA-seq) and unraveled a unique maladaptive ATM subpopulation defined as ATF4PDIA3ACSL4CCL2 inflammatory and metabolically activated macrophages (iMAMs), in which PDIA3 is required for the maintenance of their migratory and pro-inflammatory properties.

Katanin regulatory subunit B1 (KATNB1) regulates BTB dynamics through changes in cytoskeletal organization.

In this study, we have explored the role of the KATNB1 gene, a microtubule-severing protein, in the seminiferous epithelium of the rat testis. Our data have shown that KATNB1 expressed in rat brain, testes, and Sertoli cells. KATNB1 was found to co-localize with α-tubulin showing a unique stage-specific distribution across the seminiferous epithelium.

Correlation between the Systemic Immunoinflammatory Index and Platelet-Lymphocyte Ratio in Patients with Adenomyosis.

Background: The chronic inflammatory immune response is a significant factor in the pathogenesis of benign gynecological diseases. The systemic immunoinflammatory index (SII) and the platelet-to-lymphocyte ratio (PLR) are commonly available biomarkers of inflammation. However, evidence of the relationship between SII and PLR in patients with adenomyosis is limited.

Bifunctional ArsI Dioxygenase from sp. ST3 with Both Methylarsenite [MAs(III)] Demethylation and MAs(III) Oxidation Activities.

Methylated arsenicals, including highly toxic species, such as methylarsenite [MAs(III)], are pervasive in the environment. Certain microorganisms possess the ability to detoxify MAs(III) by ArsI-catalyzed demethylation. Here, we characterize a bifunctional enzyme encoded by the gene from sp.

Homotypic cell-in-cell structure as a novel prognostic predictor in non-small cell lung cancer and frequently localized at the invasive front.

Homotypic cell-in-cell structures (hoCICs) are associated with tumor proliferation, invasion, and metastasis and is considered a promising prognostic marker in various cancers. However, the role of hoCICs in non-small cell lung cancer (NSCLC) remains unclear. Tumor tissue sections were obtained from 411 NSCLC patients.

Solidification/stabilization pre-treatment coupled with landfill disposal of heavy metals in MSWI fly ash in China: A systematic review.

The growth in municipal solid waste incineration (MSWI) has resulted in a substantial rise in the production of fly ash in China. It is anticipated that during the "14th Five-Year Plan", the accumulated amount of fly ash stocked and disposed of at landfills will surpass 100 million tons. With the development of the economy and the implementation of garbage classification relevant policies, the pollution characteristics of heavy metal change in spatiotemporal distribution.

Reduced syncytin-1 regulates trophoblast invasion and apoptosis in preeclampsia.

Introduction: Preeclampsia is a pregnancy-specific disorder characterized by de novo development of hypertension and proteinuria over 20 weeks gestation that has been associated with the dysfunction of trophoblasts. Current evidence suggests that syncytin-1 plays an important role in the non-fusogenic biological activity of trophoblasts, except for specific fusogenic function. However, the underlying mechanism remains unclear.

Strategies and Mechanisms of First-Row Transition Metal-Regulated Radical C-H Functionalization.

Radical C-H functionalization represents a useful means of streamlining synthetic routes by avoiding substrate preactivation and allowing access to target molecules in fewer steps. The first-row transition metals (Ti, V, Cr, Mn, Fe, Co, Ni, and Cu) are Earth-abundant and can be employed to regulate radical C-H functionalization. The use of such metals is desirable because of the diverse interaction modes between first-row transition metal complexes and radical species including radical addition to the metal center, radical addition to the ligand of metal complexes, radical substitution of the metal complexes, single-electron transfer between radicals and metal complexes, hydrogen atom transfer between radicals and metal complexes, and noncovalent interaction between the radicals and metal complexes.

Application of metagenomic next-generation sequencing in the etiological diagnosis of refractory pneumonia in children.

Objective: Metagenomic next-generation sequencing (mNGS) was used to analyze the etiological distribution of refractory pneumonia in children. We compared its efficacy in pathogen diagnosis against traditional methods to provide a basis for clinical adjustment and treatment.

Methods: A total of 60 children with refractory pneumonia treated at the Department of Respiratory Medicine, Children's Hospital Affiliated with the Capital Institute of Paediatrics, from September 2019 to December 2021 were enrolled in this study.

Establishment and application of a rapid visualization method for detecting nucleic acid.

Background: Swift and accurate detection of , which is a prominent causative pathogen associated with seafood contamination, is required to effectively combat foodborne disease and wound infections. The gene is relatively conserved within and is primarily involved in the expression and regulation of virulence genes with a notable degree of specificity. The aim of this study was to develop a rapid, simple, and constant temperature detection method for in clinical and nonspecialized laboratory settings.