Complete Sequences and Characterization of Two Novel Plasmids Carrying aac(6')-Ib-cr and qnrS Gene in Shigella flexneri.

The complete sequences of two previously reported plasmids carrying plasmid-mediated quinolone resistance genes from Shigella flexneri in China have not been available. The present study using the p5-C3 assembly method revealed that (1) the plasmid pSF07201 with aac(6')-Ib-cr had 75,335 bp with antibiotic resistance genes CTX-M-3, TEM-1, and FosA3; (2) seven fragments of pSF07201 had more than 99% homology with the seven corresponding plasmids; (3) the other plasmid pSF07202 with qnrS had 47,669 bp with antibiotic resistance gene TEM-1 and 99.95% homology with a segment of pKF362122, which has the qnrS gene from location 162,490 to 163,146.

[Influencing factors on the voluntary premarital medical examination among Chinese population].

Objective: To explore the factors influencing the premarital medical examination in Chinese population and to discuss the related possible intervention strategies.

Methods: Data were collected through self-designed questionnaires on 2572 newly married respondents, selected under a stratified randomized sampling method, at Marriage Registration Office in 12 counties in Zhejiang province. Predictive factors were derived from the integrating complementary constructs of Health Belief Model, Theory of Reasoned Action as well as individual demographic characteristics.

[A multilevel analysis on the association between socioeconomic status and psychiatric disorders].

Objective: To explore the association between psychiatric disorders and socioeconomic status such as education, income, employment and area of community settings in Zhejiang province.

Methods: Data were from the epidemiological survey of mental illnesses aged 15 and older in 2001 (n = 14 632). Psychiatric disorders were measured by GHQ-12 with a cut-point 4.

[A multilevel cross-sectional study of mental disorder in community settings in Zhejiang Province].

Objective: To investigate the prevalence of mental disorder through the 12-item general health questionnaire (GHQ-12) survey and its related factors in community settings in Zhejiang Province.

Methods: A muti-level model was analyzed using data of 14,632 respondents nested in counties (cities), townships (urban districts), villages (urban neighborhoods) from the epidemiological survey of mental illnesses above 15 years old.

Results: The mean score measured by GHQ-12 was (1.

[Multiplex PCR assay for dissemination and diversity of extended-spectrum beta-lactamase genes in Shigella isolates].

Objective: To develop a rapid and simple multiplex polymerase chain reaction (PCR) method which discriminates extended-spectrum beta-lactamases (ESBLs) genes in sporadic Shigella isolates from 1998 to 2007 in Hangzhou city, China.

Methods: After ESBLs screening according to the Clinical and Laboratory Standards Institute (CLSI) method, CTX-M, TEM, SHV and OXA-1 encoding genes were detected by using a multiplex PCR method, and the results were verified by 8 single gene PCR amplification.

Results: Seventeen isolates harbored ESBLs genes among 195 Shigella isolates (8.