Determination of total urinary 2,5-hexanedione in the Chinese general population.

Objective: Determination of the urinary levels of 2.5-hexanedione (2,5-HD) was performed in subjects belonging to the Chinese general population to define the reference value for this metabolite.

Methods: Urine samples were collected from 8235 individuals (4216 men and 4019 women) from the healthy general population who had not been occupationally exposed to n-hexane or methyl-n-butyl ketone.

[The analysis of urinary N-methylacetamide by GC-NPD with a direct injection].

Objective: To establish a method to detect N-methylacetamide (NMAC) concentration in urine of workers occupationally exposed to NMAC with directly injecting the sample into capillary gas chromatography.

Methods: After frozen urine samples were isolated from precipitation by centrifugation, the aliquot of supernatant was pretreated by protein precipitation with dilution of methanol. The methanol supernatant was separated by Polyethylene Glycol (PEG) capillary columns and detected by nitrogen phosphorous detector (NPD).

[The impact of N, N-dimethylacetamide on the health of workers].

Objective: To explore the hepatic toxicity and the exposure biomarkers of N, N-Dimethylacetamide.

Methods: One hundred forty five objects were chosen by stratified random sampling method. The investigation was performed using questionnaire and physical examination.

[The protein expression profiles induced by trimethyltin chloride in Vero cells].

Objective: To explore the biomarkers and mechanism of kidney toxicity induced by trimethyltin chloride (TMT-Cl) through analyzing the differences of protein expression profiles between vero cells and vero cells exposed to TMT-Cl.

Methods: The differences of protein expression levels of three paired samples of vero cells and vero cells exposed to TMT-Cl were compared by two-dimensional gel electrophoresis (2-DE) and liquid chromatography-electrospray ionization-linear trap quadrupole (LC-ESI-LTQ). The differences of expression levels of Annexin A1 and α-Tubulin proteins were validated with western blot assay, and the differences of mRNA expression levels of Annexin A1 and α-Tubulin genes were detected with quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).

[The interventions effect-assessment of the workers exposed to N, N-dimethylformamide by percutaneous in a synthetic leather factory].

Objective: To assess the effects of interventions on synthetic leather workers exposed to N, N-dimethylformamide (DMF) by skin.

Methods: Twenty-six workers exposed to DMF were recruited. The level of DMF in ambient or handwash solution and N-methylformamide (NMF) in end-shift urine samples were detected before interventions and after interventions for six months.

[Determination of trimethyltin chloride in urine by headspace-gas chromatography].

Objective: To establish a detection method for trimethyltin chloride in urine by the Head space-GC.

Method: After derivatizing trimethyltin chloride, the urines was separated by the head space-gc, and then the trimethyltin chloride detected qualitatively and quantificationally.

Results: In the concentration range of 0.

[Investigation on dermal contamination among workers occupationally exposed to dimethylformamide].

Objective: To establish a method for the determination of dimethylformamide (DMF) and investigate dermal contamination and absorption among workers occupationally exposed to DMF.

Method: 37 workers exposed to DMF were divided randomly into two groups. DMF was washed down by isopropyl alcohol in A group (16 workers) and water in B group(21 workers).

[Abnormal liver function associated with polymorphism of GSTT1, GSTM1 and CYP2E1 in workers exposed to N, N-dimethylformamide].

Objective: To investigate abnormal liver function associated with polymorphism of GSTT1, GSTM1 and CYP2E1 in workers exposed to N, N-dimethylformamide.

Methods: Sixty-nine workers with abnormal liver function in a synthetic leather factory were recruited as case. One hundred and twenty five control subjects with similar work tasks were selected from the same factory.

[Biological monitoring of workers exposed to trimethyltin chloride].

Objective: To investigate suitable biomarkers for workers exposure to trimethyltin chloride (TMT-cl).

Methods: Urinary samples of 44 male workers from five TMT-cl occupational poisoning incidents were collected. Methyltin mercaptide stabilizers and waste plastics used in the incidents were also collected.

[Abnormal liver function associated with occupational exposure to N, N-dimethylformamide in synthetic leathers works].

Objective: To investigate the hepatotoxic effects of N, N-dimethylformamide (DMF) in the workers of a synthetic leathers factory, and the effects on liver function of covariates such as alcohol consumption and other factors.

Methods: The workers were classified into three groups (low, high and the control) by the concentration of DMF in workplace which was determined in the past two years. A questionnaire was drawn up for relevant demographic characteristics and other factors influencing liver function.