Publications by authors named "Ya-li She"
Matrine reduces proliferation of human lung cancer cells by inducing apoptosis and changing miRNA expression profiles.
Asian Pac J Cancer Prev
December 2014
Matrine, a main active component extracted from dry roots of Sophora flavecens , has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms of action remain unclear. To determine effects of matrine on proliferation of A549 cells and assess possible mechanisms, MTT assays were employed to detect cytotoxicity, along with o flow cytometric analysis of DNA content of nuclei of cells following staining with propidium iodide to analyze cell cycle distribution. Western blotting was performed to determined expression levels of Bax, Bcl-2, VEGF and HDAC1, while a microarray was used to assessed changes of miRNA profiles.
View Article and Find Full Text PDFObjective: To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21(Ras) and proliferating cell nuclear antigen (PCNA) gene in erythroleukemia cell line K562.
Methods: The effect of total flavonoids of Hedysarum polybotry on K562 cell line survival was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay. The time- and dose-dependent manner was also observed.
Actions of genistein on contractile response of smooth muscle isolated from guinea pig gallbladder.
Hepatobiliary Pancreat Dis Int
December 2009
Article Synopsis
- Defective contractile motility of the gallbladder contributes to gallstone formation, with estrogen potentially increasing this risk; genistein, a phytoestrogen, was tested for its effects on gallbladder smooth muscle.
- In an experiment, guinea pig gallbladders were used to measure contractile responses to varying concentrations of genistein, while also evaluating mechanisms via specific antagonists.
- Results showed that genistein decreased both the resting tension and contractile responses of gallbladder smooth muscle, suggesting it could play a role in gallstone formation by relaxing the muscle, particularly by affecting responses to acetylcholine.