Publications by authors named "Ya-fang Wu"
Article Synopsis
- - The study examined how oblique sutures impact the gapping resistance in 4-strand flexor tendon repairs using 72 porcine tendons and comparing different suture configurations.
- - Results showed no significant gap resistance differences with oblique sutures, except for the modified Savage and Adelaide sutures; Kessler-type anchorage was less effective than other anchorage types.
- - The conclusion indicates that while oblique sutures don't enhance gap resistance with Kessler-type or grasping sutures, they do improve resistance with cross-locking anchorage, like the Adelaide suture.
Article Synopsis
- Tendon-derived cells change their characteristics and lose the ability to grow after multiple cell passages in a lab setting, with specific changes observed over time.
- A new cell type that expresses periostin (Postn) was discovered during later passages, showing high proliferation and better ability to differentiate into bone tissue compared to earlier cells.
- The study analyzed various cell properties and found significant differences in gene expression between Postn cells and early passage cells, suggesting potential for further research into their use in regenerative therapies.
Article Synopsis
- The study investigates the mechanical performance of the double Q suture technique compared to the double modified Kessler suture in tendon repairs during angular motion.
- Results showed that the double Q suture had a smaller gap size and better resistance to gap formation on the dorsal aspect under curved loading conditions.
- The findings suggest that the double Q suture technique is a more effective method for preventing gap formation during flexor tendon repairs, highlighting its clinical relevance for better surgical outcomes.
Article Synopsis
- - The study examined how different speeds of movement (loading speed) affect the strength and gap resistance of repaired tendons, using porcine flexor tendons as models.
- - It was found that slower loading speeds increased the size of gaps at tendon repair sites and reduced stiffness, while faster speeds did not impact the maximum strength of the repairs.
- - These results indicate that slower motions post-surgery can lead to poorer tendon repair outcomes, suggesting a need for tailored exercise regimens to support functional recovery after tendon surgery.
Background: Tumor vascular mimicry is an emerging issue that affects patient survival while having no treatment at the current moment. Despite several factors implicated in vascular mimicry, little is known about stromal factors that modulate tumor microenvironment and shape malignant transformation. CD248, a type-I transmembrane protein dominantly expressed in stromal cells, mediates the interaction between cells and extracellular matrix proteins.
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- The study investigates the effectiveness of the Q suture as a core suture in tendon repair, comparing it to traditional sutures by varying the purchase length.
- Forty pig flexor tendons were used to test double Q sutures against Tsuge and Kessler sutures, measuring gap formation and tensile strength.
- Results suggest the double Q suture with a purchase length of 4-6 mm significantly reduced gap formation and had greater tensile strength, indicating its potential as a reliable option for tendon repairs.
Article Synopsis
- The main challenge in effective integrated pest management (IPM) is the lack of accurate and real-time data on crop damage due to pests and diseases.
- To address this, the researchers developed an Intelligent and Integrated Pest and Disease Management (I PDM) system that uses edge computing devices to automatically identify major greenhouse pests and monitor environmental conditions.
- Results showed that this system significantly aided farm managers in reducing pest populations by up to 50.7% over 1368 days, emphasizing the benefits of a data-driven approach to IPM in sustainable agriculture.
Article Synopsis
- The field of hand surgery is adapting to the needs of an aging population and lifestyle demands for more active living.
- While surgical techniques have improved over the years, future advancements are expected to come from biologics and basic science discoveries.
- The article discusses how basic science solutions can address significant challenges in hand surgery, particularly in enhancing tissue repair.
The impact of transverse components on resistance and ultimate strength of 6-strand tendon repairs.
J Hand Surg Eur Vol
October 2021
Article Synopsis
- - The study evaluated how the transverse components of tendon core sutures impact the strength of 6-strand tendon repairs, specifically comparing Tang and Yoshizu #1 methods with 4-strand repairs.
- - A total of 40 pig flexor tendons were tested under cyclic loading to measure gap formation, stiffness, and ultimate repair strength after 20 cycles.
- - Results showed that Yoshizu #1 repairs had a higher tendency to develop gaps and were significantly weaker compared to Tang repairs, highlighting the influence of transverse components on repair integrity.
We investigated the effects of the transverse components of a tendon core suture on tensile resistance and strength of 4-strand repairs. Forty-four pig flexor tendons were repaired with one of the following four methods: double Tsuge, U-shaped, 4-strand cross and 4-strand rectangular repairs. We recorded the number of the repaired tendons that formed a 2 mm gap between the tendon ends during cyclic loading for 20 cycles, stiffness of the tendon at the 1st and 20th cycle, gap distance at the repair site and ultimate strength of the repair at the 20th cycle.
View Article and Find Full Text PDFThe Effects of Hypoxia-Reoxygenation in Mouse Digital Flexor Tendon-Derived Cells.
Oxid Med Cell Longev
September 2021
Article Synopsis
- Ischemia-reperfusion injury causes severe tissue damage when blood flow is restored after a lack of oxygen, and this study used a hypoxia-reoxygenation model to explore its effects on tendon cells from mice.
- Tendon cells were treated with varying concentrations of cobalt chloride to simulate different H/R conditions over 24 hours followed by reoxygenation for up to 96 hours, with multiple assays used to assess cell viability, growth, oxidative stress, and apoptosis markers.
- Results showed that while cell viability increased notably with both low and high cobalt treatment after reoxygenation, mitochondrial activity rose, oxidative stress was induced, and there were contrasting effects on specific protein expressions, indicating a complex response to H/R conditions.
Article Synopsis
- No prior research thoroughly investigated the immune response in the intestines of yellow-stage European eels when infected by Aeromonas hydrophila, leading to this study using time-resolved RNA-seq at three different time points post-infection.
- Results showed a significant immune response, particularly through the "cytosolic DNA-sensing pathway," with certain genes (like irf3 and tlr1) being highly expressed at the early stages, but not later.
- The findings highlight the complex immune mechanisms in eels, including the unique roles of specific genes and proteins, which could inform further research on host-pathogen interactions.
Article Synopsis
- Peripheral epitendinous sutures are used in tendon repairs to improve strength and reduce gaps, but the Q suture is proposed as a viable alternative.
- A study evaluated the effectiveness of Q sutures compared to traditional running peripheral sutures by testing various configurations on porcine tendons.
- Results showed that Q sutures led to fewer gaps during loading, increased ultimate strength in repairs, and required less time to implement than running sutures, indicating their potential as a better option for tendon repair.
Article Synopsis
- Tendon stem/progenitor cells (TSPCs) show promise for tendon regeneration, but distinguishing them from tenocytes is challenging based on seeding density.
- In the study, tendon-derived cells from mice were seeded at varying densities, revealing that low densities (50 and 500 cells/cm) formed distinct colonies, while higher densities (5,000 and 50,000 cells/cm) did not.
- Results indicated that while there was a positive correlation between cell proliferation and density, higher density led to less pronounced stem cell characteristics, suggesting that isolating pure TSPCs through density gradients is ineffective.
Article Synopsis
- The study aimed to understand the gene expression profiles of tendon healing after injury, focusing on the flexor digitorum profundus tendons in a chicken model.
- Analysis revealed three distinct phases of healing, with significant involvement of cell proliferation and adhesion processes, as well as key signaling pathways related to cytokines and extracellular matrix interactions.
- Overall, the findings provide insights into the molecular mechanisms of tendon repair, which could help identify potential targets for improving tendon healing through genetic engineering.
Article Synopsis
- The study focused on improving the repair of digital flexor tendons after laceration by testing a simple Q suture technique to prevent gapping and restore tensile strength.
- Sixty porcine tendons were used in experiments to compare different suture methods, with results showing that Q sutures significantly reduced gap formation and increased the ultimate strength of the repairs.
- The findings indicate that the Q suture technique is effective in enhancing tendon repair resistance to gapping and improving strength, making it clinically relevant for tendon repair procedures.
Suture knots between tendon stumps may not benefit tendon repairs.
J Hand Surg Eur Vol
November 2018
Tendon injury during limb motion is common. Damaged tendons heal poorly and frequently undergo unpredictable ruptures or impaired motion due to insufficient innate healing capacity. By basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF) gene therapy via adeno-associated viral type-2 (AAV2) vector to produce supernormal amount of bFGF or VEGF intrinsically in the tendon, we effectively corrected the insufficiency of the tendon healing capacity.
View Article and Find Full Text PDFGene therapy strategies to improve strength and quality of flexor tendon healing.
Expert Opin Biol Ther
October 2016
Article Synopsis
- Tendon surgery often faces challenges with repair and adhesion issues, leading to a focus on new biological therapies to enhance healing.
- This review highlights the use of adeno-associated viral (AAV) type 2 vector gene therapy and micro-RNA therapies in a chicken model to improve tendon healing and minimize adhesions.
- Initial results show that AAV2 can strengthen tendon healing and reduce adhesions, with ongoing work needed to optimize delivery methods and understand adhesion formation better.
Article Synopsis
- * Researchers created polylactic-co-glycolic acid nanospheres filled with plasmids to deliver green fluorescence protein and miRNA targeting the transforming growth factor-β1 gene.
- * The study found that these nanosphere/plasmid combinations effectively reduced transforming growth factor-β1 in healing chicken tendons without causing inflammation or tissue damage.
Purpose: To evaluate the biomechanical properties of an asymmetric core suture for tendon repair.
Methods: Sixty porcine flexor tendons were repaired with 3 different 4-strand sutures using different core suture purchases: 2 sets of identical purchases of 10 mm, 2 sets of asymmetric purchases (8 mm proximal/distal stump and 12 mm distal/proximal stump), and 2 sets of identical purchases of 12 mm. The tendons were subjected to the cyclic loading for 20 cycles.
[Clinical and experimental characteristics of 20 patients with acute myeloid leukemia with complex variant of t(8; 21)].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
August 2013
This study was aimed to summarize and analyze the morphology, immunophenotype, cytogenetics, molecular biology (MICM), tyrosine kinase (TK) gene mutations and clinical features of acute myeloid leukemia (AML) with complex variant of t(8;21). A retrospective study was performed for 20 AML patients with complex variant of t(8;21) in our hospital from January 1994 to April 2012, including analysis of clinical feature, immunophenotype, chromosome karyotype, treatment regimen, as well as the overall survival (OS) and relapse-free survival (RFS). Mutations of C-KIT, FLT3-ITD, FLT3-TKD and JAK2V617F were detected by genomic DNA PCR and the sequencing was per-formed in 13 AML patients with complex variant of t(8;21).
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