Involvement of Holotrichia parallela odorant-binding protein 3 in the localization of oviposition sites.

Organic fertilizers-derived volatiles attract Holotrichia parallela during oviposition. However, the mechanisms underlying the perception of oviposition cues in H. parallela remain unclear.

PI3K/Akt/CncC signaling pathway mediates the response to EPN-Bt infection in Holotrichia parallela larvae.

Background: Combining the entomopathogenic nematode (EPN), Heterorhabditis beicherriana LF strain, and Bacillus thuringiensis (Bt) HBF-18 strain is a practical strategy to manage the larvae of Holotrichia parallela Motschulsky (white grubs). However, the mechanisms underlying the larval defense response to this combined biocontrol strategy are unknown.

Results: The activities of some antioxidant enzymes (SOD, POD, CAT) and some detoxifying enzymes (AChE, P-450, CarE, GST) in grubs showed an activation-inhibition trend throughout the EPN-Bt exposure time course.

Molecular characterization of sex pheromone binding proteins from Holotrichia oblita (Coleoptera: Scarabaeida).

Pheromone binding proteins (PBPs), a subfamily of the odorant binding proteins (OBPs), capture and transfer sex pheromones across the sensillum lymph to pheromone receptors and initiate insect courtship and mating. In this study, we functionally characterized ten OBPs from the black chafer, Holotrichia oblita (HoblOBPs), among which six HoblOBPs (HoblOBP2, 4, 5, 8, 9 and 24) were shown to recognize sex pheromones using electroantennography assays (EAG) and in vitro fluorescence competitive binding assays. Insect tropism to sex pheromones was significantly reduced after those genes were knocked down in vivo, e.

[Behavioral responses of Holotrichia oblita larvae to root exudates from three host plants].

The larva of Holotrichia oblita is a serious soil pest that feed with plant roots in north China. To explore the effects of host root exudates on the larva could provide theoretical basis for the development of green prevention and control methods. In order to elucidate the behavioral responses of Holotrichia oblita larva to the roots of peanut, soybean and maize, an experiment was conducted using the Y-olfactometers with the air as control.

Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus.

Loxostege sticticalis Linnaeus is an economically important agricultural pest, and the larvae cause great damage to crops, especially in Northern China. However, effective and environmentally friendly chemical methods for controlling this pest have not been discovered to date. In the present study, we performed HiSeq2500 sequencing of transcriptomes of the male and female adult antennae, adult legs and third instar larvae, and we identified 54 candidate odorant receptors (ORs), including 1 odorant receptor coreceptor (Orco) and 5 pheromone receptors (PRs), 18 ionotropic receptors (IRs), 13 gustatory receptors (GRs), 34 odorant binding proteins (OBPs), including 1 general odorant binding protein (GOBP1) and 3 pheromone binding proteins (PBPs), 10 chemosensory proteins (CSPs) and 2 sensory neuron membrane proteins (SNMPs).

Complete mitochondrial genome of Chilo suppressalis (Walker) (Lepidoptera: Crambidae).

The complete sequence of the mitochondrial genome (mitogenome) of the rice stem borer Chilo suppressalis (Walker) (Lepidoptera: Crambidae) was determined to be 15,465 bp. It contains 13 protein-coding genes (PCGs), 22 tRNA genes, the large and small rRNA genes, and an A+T-rich region. The nucleotide composition of the mitogenome of C.

Mitochondrial genome of the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) and comparison with other Lepidopterans.

We present the complete sequence of the mitochondrial genome (mitogenome) of the cotton bollworm Helicoverpa armigera. The 15,347-bp mitogenome of H. armigera was arranged in the same order described for all other sequenced lepidopterans, which differs from the most common type found in insects, due to the movement of trnM to a position 5'-upstream of trnI.

Identification and molecular characterization of a new member of the peritrophic membrane proteins from the meadow moth, loxostege sticticalis.

The peritrophic membrane (PM) plays an important role in protecting insects. The PM proteins are important to determinate the formation and function of the PM. A new PM protein, named Lsti99, was identified from the PM of Loxostege sticticalis larvae by cDNA library screening.

Detoxification of gramine by the cereal aphid Sitobion avenae.

Secondary metabolites play an important role in host plant resistance to insects, and insects, in turn, may develop mechanisms to counter plant resistance mechanisms. In this study, we investigated the toxicity of gramine to the cereal aphid Sitobion avenae and some enzymatic responses of S. avenae to this alkaloid.