Discovery of soluble epoxide hydrolase inhibitors from Inula britannica: Inhibition kinetics, molecular dynamics simulation, biochemical, and in vitro cell-based studies.

Soluble epoxide hydrolase (sEH) is an enzyme involved in transforming epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatrienoic acids (DHETs). EETs play a pivotal role in maintaining anti-inflammatory response and pain regulation, making sEH as an important target for therapeutic interventions. Based on our present study of discovery of sEH inhibitors, herein, six new (1-6) and fifteen known compounds (7-21) with inhibitory effects toward sEH were isolated from Inula britannica.