Altered salivary microbiota profile in patients with abdominal aortic aneurysm.

Unlabelled: Evidence suggests that the DNA of oral pathogens is detectable in the dilated aortic tissue of abdominal aortic aneurysm (AAA), one of the most fatal cardiovascular diseases. However, the association between oral microbial homeostasis and aneurysm formation remains largely unknown. In this study, a cohort of individuals, including 53 AAA patients and 30 control participants (CTL), was recruited for salivary microbiota investigation by 16S rRNA gene sequencing and bioinformatics analysis.

Periodontitis exacerbates atherosclerosis through Fusobacterium nucleatum-promoted hepatic glycolysis and lipogenesis.

Aims: Positive associations between periodontitis (PD) and atherosclerosis have been established, but the causality and mechanisms are not clear. We aimed to explore the causal roles of PD in atherosclerosis and dissect the underlying mechanisms.

Methods And Results: A mouse model of PD was established by ligation of molars in combination with application of subgingival plaques collected from PD patients and then combined with atherosclerosis model induced by treating atheroprone mice with a high-cholesterol diet (HCD).

Roles of oral microbiota and oral-gut microbial transmission in hypertension.

Introduction: Considerable evidence has linked periodontitis (PD) to hypertension (HTN), but the nature behind this connection is unclear. Dysbiosis of oral microbiota leading to PD is known to aggravate different systematic diseases, but the alteration of oral microbiota in HTN and their impacts on blood pressure (BP) remains to be discovered.

Objectives: To characterize the alterations of oral and gut microbiota and their roles in HTN.

Characteristics and Correlations of the Oral and Gut Fungal Microbiome with Hypertension.

The mycobiome is an essential constituent of the human microbiome and is associated with various diseases. However, the role of oral and gut fungi in hypertension (HTN) remains largely unexplored. In this study, saliva, subgingival plaques, and feces were collected from 36 participants with HTN and 24 healthy controls for metagenomic sequencing.

Endoplasmic reticulum stress response mediated by the PERK-eIF2α-ATF4 pathway is involved in odontoblastic differentiation of human dental pulp cells.

Objective: RNA-activated protein kinase-like ER-resident kinase (PERK) was a major transducer of Endoplasmic reticulum (ER) stress response and it directly phosphorylated α-subunit of eukaryotic initiation factor 2 (eIF2α), which specifically promoted the translation of activating transcription factor 4 (ATF4), an important transcription factor in cells' differentiation. The purpose of this study was to establish whether ER stress mediated by PERK-eIF2α-ATF4 pathway was involved in odontoblastic differentiation of human dental pulp cells (DPCs).

Methods: DPCs were isolated from extracted teeth and cultured in odontogenic medium.

[The risk and prevention of bio-aerosol transmission in dental clinics].

Corona virus disease 2019 (COVID-19) has become a Public Health Emergency of International Concern since its outbreak, and whether COVID-19 can transmit by aerosol remains controversial. The problem of bio-aerosol transmission in the relatively confined dental clinics has aroused wide attention in the field of dentistry. This review provided a most updated summary on the relation between bio-aerosols and dental clinics, which included the microorganisms in bio-aerosols, the bio-aerosol transmission and the sources testing methods, temporal and spatial distribution of dental bio-aerosols and summarized how to reduce the exposure to bio-aerosols in dental clinics.

[Management and prevention of dental emergency during corona virus disease 2019 (COVID-19) epidemic].

The outbreak of corona virus disease 2019 (COVID-19) has developed rapidly and the situation of prevention and control is severe. During the epidemic period of COVID-19, due to the particularity of diagnosis and treatment of oral diseases, there is great challenges for how to deal with various types of dental emergency. In order to prevent and control the epidemic situation strictly, and to perform a scientific and orderly clinical diagnosis and treatment of dental emergency, this article provided suggestions on personnel management training, procedures and treatment, protection and disinfection of dental emergency during COVID-19 epidemic, and reference for dental institutions and medical staff.

[Effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells].

Purpose: To compare the effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells (hDPCs).

Methods: HDPCs were cultured to 80% and 100% confluence respectively, and then cultured for 24, 48 and 72 hours by culture medium containing 0.5% fetal bovine serum(FBS).

Effect of Lactobacillus acidophilus and Porphyromonas gingivalis on proliferation and apoptosis of gingival epithelial cells.

Purpose: This study aimed to evaluate the possible antagonistic effects of Lactobacillus acidophilus on Porphyromonas gingivalis, and detect inhibition of Lactobacillus acidophilus on Porphyromonas gingivalis when they are co-cultured with human gingival epithelial cells.

Materials And Methods: Human gingival epithelial cells were co-cultured with Lactobacillus acidophilus and Porphyromonas gingivalis alone or together. The amount of Porphyromonas gingivalis adhering to or invading the epithelial cells were determined by bacterial counts.

[Effect of pulsed dye laser on inhibiting early scar after maxillofacial trauma].

Purpose: The study was designed to explore an effective method to control early scar after maxillofacial trauma and improve the satisfaction of clinical treatment.

Methods: Fifty skin lesions after maxillofacial trauma were divided into the experimental group and control group. Patients in the experimental group were treated with pulsed dye laser when taking out stitches, 15, 30 and 60 days later.

[Establishment of a model of endoplasmic reticulum stress response in dental pulp cells induced by tunicamycin].

Purpose: The aim of this study was to establish a model of endoplasmic reticulum (ER) stress in dental pulp cells(DPCs) induced by tunicamycin to better understand the molecular mechanism of DPCs related diseases mediated by ER stress.

Methods: DPCs were cultured using modified tissue explant technique in vitro and cultured in presence or absence of tunicamycin. DPCs' viability was measured by methylthiazol tetrazolium (MTT) assay.

[Evaluation of effects of fluoride varnish on prevention of first permanent molar caries].

Purpose: To compare fluoride varnish and fluoride foam's effectiveness of preventing first permanent molar caries, and discuss patients' acceptance of the 2 materials among pupil patients.

Methods: Six hundred pupils aged 6-8 years from 3 primary schools in Shanghai Yangpu District were randomly selected.The subjects were randomly divided into 3 groups, with 200 students having 800 teeth in each.

[Comparison of the accuracy of measuring root canal working length in 3 different ways].

Purpose: To compare the accuracy of measuring root canal working length with two different electronic apex locators and digital X-ray.

Methods: Ninety patients treated from January 2015 to June 2016 were selected, 232 root canals in total were randomly divided into 3 groups: X-ray group, Raypex5 group and i-root group. The accuracy of working length measured was assessed according to the filling quality of related main tips.

[Construction of a lentiviral vector of RNA interference of PERK gene and identification in human dental pulp cells].

Purpose: To construct an expression vector of a small hairpin RNA (shRNA) targeting human PERK gene and to observe gene-silencing effects of PERK in human dental pulp cells (DPCs).

Methods: According to PERK gene cDNA sequence, shRNA was designed and synthesized, which was then annealed into hU6-MCS-CMV-EGFP vector. After identified by sequencing, hU6-MCS-CMV-EGFP vector and packaging vector were co-transfected into 293 T cells.

[Comparative evaluation of marginal microleakage of three different resins in Class V composite restorations].

Purpose: To compare and evaluate microleakage at the occlusal wall and cervical wall in Class V cavities restored with Ivoclar Tetric N Ceram Bulk Fill composite, Tetric N Flow composite and N Ceram nanocomposite.

Methods: Sixty-six extracted human maxillary premolars, which were intact and healthy, were randomly assigned into 3 groups (n=22). Standardized Class V cavities were prepared on the buccal surface of maxillary premolars.

[Elimination of Entercoccus faecalis with different disinfection methods in root canals in vitro].

Purpose: To compare the elimination effect against E.faecalis in root canals with different methods.

Methods: Fifty extracted premolars with single root canal were selected.

Differential expression between "DSP-only" and DSP-PP transcripts in rat molar teeth.

Objective: To compare the expression patterns of two multiple transcripts derived from DSP-PP gene during tooth development. One is DSP-only transcript (i.e.

[Clinical management of calcified root canal with Mtwo NiTi files].

Purpose: To evaluate the effect of Mtwo NiTi files in the management of calcified root canals.

Methods: Thirty-five calcified root canals from 21 cases unsuccessfully prepared with C-Pilot files and EDTA were treated with Mtwo NiTi files and EDTA. The data was analyzed with SAS 9.

[Influence of the root canal instrumentation size on the disinfection of intracanal microbe by Er:YAG laser].

Purpose: To evaluate the influence of different root canal instrumentation size on disinfection of intracanal microbe of dental root canal.

Methods: 368 extracted human anterior teeth with single straight root were randomly divided into 8 groups of 46 roots in each. They were instrumented with K3 Ni-Ti files as follows: group A1 and group B1(#25/0.

[Study of the root canal characteristics of mandibular central incisors in 150 patients using cone-beam computed tomography].

Purpose: To investigate the root canal characteristics of mandibular central incisors using a cone-beam computed tomography (CBCT) technique.

Methods: One hundred and fifty patients (79 males and 71 females) receiving CBCT scan at Shanghai Ninth People's Hospital were recruited into this study. A total of 295 image samples of mandibular central incisors were obtained and analyzed in terms of the number and type of root canals.

Geometric analysis of root canals prepared by single twisted file in three different operation modes.

Objective: The aim of this study was to evaluate and compare the effects of a single twisted file (TF) instrument in three different operation modes on the preparation of curved root canals in human molars and to explore a new possible method in canal shaping in the clinic setting.

Materials And Methods: A total of 105 selected root canals with an angle of curvature ranging from 20° to 35° were divided into the following three groups with 35 samples each according to the different operation mode in canal preparation: "continuous rotation-500" (CR, 500 rpm), reciprocating movement-300 (RM-300 rpm) and CR-300 rpm. Root canals were prepared by single file (a size 25/0.